LOCUS       sequence-396173-       13645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13645)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1207
FEATURES             Location/Qualifiers
     source          1..13645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7672..7683
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    9502..10090
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9555..9575)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9973..9984)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10162..10395
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10473..10594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10510..10529)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10564..10583
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10634..10769
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10789..10808)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10790..10808)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10818..10834)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10827..10849)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10976..11431
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11063..11082)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11273..11294
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11457..11561
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11562..11630,11631..12422)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11780..11799)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12593..13181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13082..13101
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13335..13352
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13469..13490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13505..13510,13511..13528,13529..13535)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13543..13559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13548..13570
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13567..13583
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13567..13583
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13602..13622
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13604..13622
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggat cttggaacag ctgagggcac
     4141 ccggtgcacg gacccgcctg caggcaagcc cgccatggcg cccaaacgca agggtggcct
     4201 gaagctgaac gccatctgcg ccaagctgag ccgccaggtg gtggtggaga agcgagctga
     4261 cgccggctcc cacacggagg gcagcccatc gcagccccgg gaccaagagc gcagtggccc
     4321 tgagtctggg gcagcccggg ccccccgcag cgaggaagac aagagacggg cagtgatcga
     4381 gaagtgggtg aacggggagt acagcgagga gccggcaccc acacccgtgt tggggcggat
     4441 tgcccgcgag ggcctggagc tgcctcccga gggtgtctac atggtgcagc cccaggggtg
     4501 cagcgatgag gaagaccacg cggaggagcc ctccaaggac ggcggtgccc tggaggagaa
     4561 ggattcggac ggggcagcct ccaaggagga cagcggcccc agcaccaggc aggcttcagg
     4621 agaggcctcc tcgctgcggg actacgcggc ctccaccatg accgagttcc tcggcatgtt
     4681 tggctatgat gaccagaaca cgcgggacga gctggccagg aagatcagct ttgagaagct
     4741 gcacgcgggc tccaccccgg aggcagccac ctcctccatg ctgcccacct ccgaggatac
     4801 cctcagcaag cgggcgcggt tctctaagta tgaggagtac atccgcaagc tcaaggctgg
     4861 cgagcagctc tcctggccgg cccccagcac caagaccgag gagcgggtgg gcaaggaggt
     4921 ggtgggcacc ctgcccggcc tgcggctgcc cagcagcacg gcccacctgg agaccaaggc
     4981 caccatcctg cccctgccgt cgcacagcag tgtccagatg cagaacctgg tagcccgggc
     5041 ctccaagtac gacttcttca tccaaaaact gaagaccggc gagaatctgc ggccccagaa
     5101 cgggagcacc tacaagaagc catccaagta cgacctggag aatgtcaagt acctgcacct
     5161 cttcaaaccc ggggagggca gccccgacat gggcggggcc atcgccttca agacaggcaa
     5221 ggtggggcgc ccttccaagt acgacgtccg gggcatccag aagccaggcc ccgccaaggt
     5281 tccgcccacc cccagcctgg ctcccgcacc cctcgccagc gtgcccagtg cccccagcgc
     5341 ccccgggcca gggccagagc ctcctgcctc cctgtccttc aacactcccg agtacctgaa
     5401 gtcaaccttc tccaaaacag actccatcac cacggggacc gtctccactg tcaagaacgg
     5461 actgcccaca gataaaccag ccgtcactga agatgtaaac atttaccaga aatatattgc
     5521 caggttctcg ggcagccagc actgtggcca catccactgt gcctaccagt accgcgagca
     5581 ctaccactgc cttgaccctg agtgtaacta ccagaggttc acgagtaagc aggacgtgat
     5641 ccgccactac aacatgcaca agaagcgcga caactccctg cagcacggct tcatgcgttt
     5701 cagcccgctg gacgactgca gcgtctacta ccacggctgc cacctcaatg ggaagagcac
     5761 ccactatcac tgcatgcagg tgggctgtaa caaggtgtac acgagcacgt ctgacgtgat
     5821 gacccacgag aacttccaca agaagaatac ccagctcatt aacgacggct tccagcgctt
     5881 ccgagccacc gaagactgtg gcacagccga ctgccagttc tacggacaga agaccacgca
     5941 cttccactgc aggcgccccg gctgcacatt cactttcaag aacaagtgtg acatcgagaa
     6001 gcacaagagc taccacatca aggacgatgc ctacgccaag gacggcttca agaagttcta
     6061 caagtacgag gagtgcaagt acgagggctg cgtgtacagc aaggctacca accacttcca
     6121 ctgcatccgc gccggctgcg gcttcacctt cacctccacc agccagatga cctctcacaa
     6181 gcgcaagcat gagcgccggc acatccgctc ctcgggcgcg ctggggctgc cgccctcgct
     6241 gctgggcgcc aaggacacgg agcacgagga gtccagcaac gacgaccttg ttgacttctc
     6301 cgccctgagc agcaagaact ccagcctgag cgcctcccct accagccagc agtcctctgc
     6361 gtccctggct gccgccactg ccgccaccga ggctgggccc agtgccacca aacctcccaa
     6421 cagcaagatc tcggggctgc tgccccaggg cctgcctggc tcaatccccc tggccctggc
     6481 cctctccaac tcgggcctgc ccacccccac gccctacttc cccatactgg ctggccgtgg
     6541 gagcacctcc ctgcctgtgg gcacccccag cctcctgggt gccgtgtcgt ctgggtcagc
     6601 agcctcagcc acccctgaca cacccacgct ggtcgcctcg ggagctggag actcagcccc
     6661 cgtggctgcc gcctctgtcc cggcaccacc cgcctccatc atggagagga tctctgcaag
     6721 caagggcctc atctcgccca tgatggccag gctggctgca gctgccctca agccctctgc
     6781 cacctttgac ccaggaagcg ggcagcaggt caccccagcc aggttccccc cggcccaagt
     6841 gaagccggaa cccggtgaga gcaccggcgc cccaggcccc cacgaagcct cccaggaccg
     6901 cagtctagac ctgactgtga aggagcccag caacgaatca aatggccacg cagtcccggc
     6961 aaattcatct cttttatcct cgcttatgaa taagatgtct cagggcaacc ctggcctggg
     7021 cagcctgctg aacatcaagg cggaagcgga ggggagcccc gctgcggagc cctcgccctt
     7081 cctaggcaag gccgtgaagg cgctggttca ggagaagttg gcagagccct ggaaggtgta
     7141 cctgcgcagg tttggtacaa aggacttctg tgacggccag tgtgacttcc tccacaaggc
     7201 ccacttccac tgcgtggtgg aggaatgcgg cgcgctcttc agcaccttgg acggggccat
     7261 caagcacgca aacttccact tccggacaga gggaggagca gcaaaaggaa acacagaggc
     7321 tgcctttccg gcctcggccg ccgagaccaa acctcccatg gccccctcgt cccctccggt
     7381 ccctcctgtc accacggcca cggtgtcctc tctggagggg cccgctccca gcccggcctc
     7441 cgtgccctcc acccccaccc tgctcgcctg gaagcagctg gcttccacca taccccagat
     7501 gcctcagatc ccagcgtcag tgcctcacct gcccgcctcg cccttggcaa cgacttctct
     7561 agagaacgcc aagccccagg tcaaacccgg attcctccag ttccaggaga acgatccttg
     7621 cctcgccacg gactgcaagt acgccaacaa gttccacttc cactgtctct ttgggaactg
     7681 caagtacgtc tgcaaaacgt ctggcaaggc cgaatcccac tgcctggacc acatcaaccc
     7741 caacaacaac ctggtgaacg tgcgagacca gtttgcatac tactctctgc agtgtctctg
     7801 tcccaaccag cactgcgagt tccgaatgcg tgggcactac cactgcctcc gcaccggctg
     7861 ctattttgtg accaacatca ccaccaagct cccctggcac atcaagaagc atgagaaggc
     7921 ggagcggcgg gcagccaatg gcttcaaata cttcaccaag cgcgaggagt gtggcaggct
     7981 aggttgcaag tacaaccagg tgaacagcca cttccactgc atccgggagg gctgccagtt
     8041 ctccttcctc ctcaagcacc agatgacctc ccacgcgcgg aagcacatgc ggaggatgct
     8101 ggggaagaac ttcgaccgcg tgcccccctc ccagggcccc ccaggcctga tggatgctga
     8161 gacagatgag tgcatggact acactggctg cagcccaggc gccatgtcct ctgagtcatc
     8221 caccatggac cggagctgct ccagcacccc cgtgggtaac gagagcaccg cggcagggaa
     8281 caccatctct atgccgacag cctcgggggc caaaaagcgc ttctggatca tcgaggacat
     8341 gtcgcccttc ggcaagcggc ggaagacggc gtcctcccgg aagatgctgg acgagggcat
     8401 gatgctggag ggcttccggc gcttcgacct ttacgaggac tgcaaggacg cagcttgtca
     8461 gttctcgctc aaggtcaccc actaccactg cacgcgcgag aactgcggct acaagttctg
     8521 cgggcgcacg cacatgtaca agcacgcgca gcaccacgac cgcgtggaca acctggtgct
     8581 ggacgacttc aagcgcttca aggcctcact cagctgccac ttcgccgact gccccttctc
     8641 gggcaccagc acgcacttcc actgcctgcg ctgccgcttc cgctgcaccg acagcaccaa
     8701 ggtcacggcg catcgcaagc accacggcaa acaggacgtg atcagcgccg cgggcttctg
     8761 ccagttcagc tccagcgccg actgcgccgt gcccgactgc aagtacaagc tcaagtgctc
     8821 gcacttccac tgcaccttcc cgggctgccg ccacacggtg gtgggcatgt cgcagatgga
     8881 ctcgcacaag cgcaagcacg agaagcagga gcgcggcgag cccgcggcag agggccccgc
     8941 gcccgggcct cccatcagtc tggacggctc cctgtcgctg ggcgccgagc cgggctcgct
     9001 gctcttcctg cagtcggcgg ccgccggcct gggcctggcg ctgggcgacg cgggcgaccc
     9061 cggcccgccc gacgccgccg cccccgggcc gcgcgagggc gccgccgccg ccgccgccgc
     9121 agctggggag tcctcgcagg aggacgagga ggaggagctg gagctgccgg aggaggaggc
     9181 cgaagacgac gaggacgagg acgacgacga ggacgacgac gacgaggacg acgacgagga
     9241 cgacgacgac gaggacctgc gcaccgactc ggaggagtcg ctgcccgagg cggcggcgga
     9301 ggcggcgggc gcgggcgcgc ggaccccggc cttggcggcg ctggcggccc tgggcgcccc
     9361 cggccccgcg cccactgcag cctcctcgcc ctagtaatga actagtccgt aacttgaaag
     9421 tatttcgatt tcttggcttt atatatcttg tggaaaggac gacacagtca ggctgcgagc
     9481 catgtgacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     9541 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     9601 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     9661 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     9721 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     9781 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     9841 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     9901 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     9961 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
    10021 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
    10081 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
    10141 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
    10201 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    10261 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    10321 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    10381 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
    10441 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
    10501 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    10561 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
    10621 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    10681 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    10741 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
    10801 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    10861 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    10921 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
    10981 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    11041 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    11101 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    11161 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    11221 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    11281 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    11341 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    11401 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
    11461 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    11521 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    11581 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    11641 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    11701 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    11761 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    11821 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    11881 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    11941 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    12001 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    12061 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    12121 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    12181 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    12241 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    12301 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    12361 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    12421 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    12481 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    12541 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    12601 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    12661 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    12721 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    12781 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    12841 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    12901 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    12961 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    13021 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    13081 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    13141 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    13201 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    13261 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    13321 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    13381 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    13441 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    13501 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    13561 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    13621 gggaacaaaa gctggagctg caagc
//
