LOCUS       sequence-396174-       12262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12262)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1155
FEATURES             Location/Qualifiers
     source          1..12262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             4287..4295
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    8119..8707
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8172..8192)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8590..8601)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8779..9012
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9090..9211
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9127..9146)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9181..9200
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9251..9386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9406..9425)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9407..9425)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9435..9452)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9435..9451)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9444..9466)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9593..10048
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9680..9699)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9890..9911
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10074..10178
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10179..10247,10248..11039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10397..10416)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11210..11798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11699..11718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11952..11969
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12086..12107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12122..12127,12128..12145,12146..12152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12160..12176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12165..12187
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12184..12200
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12184..12200
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12219..12239
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12221..12239
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggat gatccattca gcccctgcag
     4141 gaggctgaac agtacccagg gggagattcg tgtcggtcct agtcatcagg ccaaacttcc
     4201 agatctgcaa ccatttcctt ctccagatgg tgatacagtg acccaacatg aggaactggt
     4261 ctggatgcct ggagttaacg actgtgacct ccttatgtac ttgagggcag caaggagcat
     4321 ggcggcattt gcaggaatgt gtgatggagg ctctacagag gacggctgtg tcgcagcctc
     4381 tcgggatgac accactctga atgcactgaa cacactgcat gaaagcggtt acgatgctgg
     4441 caaagccctg cagcgcctgg tgaagaagcc tgtgcccaag ctcatcgaga agtgctggac
     4501 cgaggacgaa gtgaaacgct tcgttaaggg actcaggcag tacgggaaga acttcttcag
     4561 aattagaaag gagctgcttc ccaataagga aacaggggag ctgatcacct tctattacta
     4621 ttggaagaag acccccgaag cagccagctc ccgagcccat cgtaggcacc gcaggcaggc
     4681 cgtgttcagg aggattaaga ctcgcaccgc gtccacaccc gtcaacacac cctccagacc
     4741 cccgtccagt gaattcttgg acctaagttc agccagtgaa gatgacttcg acagtgagga
     4801 cagtgagcag gagctgaagg ggtacgcctg ccgccactgc ttcaccacca cctccaaaga
     4861 ttggcaccac ggaggccggg agaacatcct gctttgcacc gactgtcgca tccacttcaa
     4921 gaaatacggt gagctcccgc ccattgagaa gcccgtggac ccgccaccgt ttatgttcaa
     4981 acccgtcaag gaagaggatg atgggctcag tgggaagcat agcatgagga cacggcggag
     5041 tcggggctcg atgtcgacac tacgcagtgg tcggaagaag cagccagcca gccctgatgg
     5101 tcgcacctca cccatcaatg aagacatccg ctccagcggc cggaactccc ccagcgctgc
     5161 cagtacctcc agcaatgaca gtaaagcaga gacagtgaag aagtcggcca agaaggtgaa
     5221 ggaggaagcc tcttcccctc ttaagagtaa caaacgccag cgggagaagg tggcctctga
     5281 tacggaggag gctgacagga ccagctccaa gaagacaaaa acgcaggaga tcagcaggcc
     5341 caactcgcca tctgaaggtg agggagagag ttcagacagt cgcagcgtca acgatgaggg
     5401 tagcagtgac cccaaagaca tcgaccagga caatcgcagc acgtccccga gcatccccag
     5461 cccccaggac aatgagagtg actcggactc gtcagcccag cagcagatgc tgcaggccca
     5521 gcccccagcc ttgcaggctc ccactggggt caccccagct ccctcctcag ctcctccagg
     5581 gacccctcag ctgcccacgc cagggcccac gccctctgcc actgcagttc ccccacaggg
     5641 ctcccccacg gcctcccagg cccctaacca gccacaggct cccacagcgc ctgttcccca
     5701 cacccacatc caacaggcac cggccttgca cccccagcgg ccgccctcac cgcatccccc
     5761 gccgcatccc tcgccacatc ccccgctgca gcctctgact gggtcggcgg gccagccttc
     5821 tgcaccctct catgcccagc ccccactgca cggtcagggc ccacccggcc ctcacagcct
     5881 gcaggctggg cccctgctgc agcacccagg ccccccacag ccctttggcc tccctcccca
     5941 ggcctcccaa ggccaggccc ctctggggac ctccccagca gcagcgtacc ctcacacctc
     6001 cctgcagctg ccagcctctc agtcagcgct gcagtcccaa cagcctccac gggagcagcc
     6061 cctgccacca gcgcccttgg ccatgcccca catcaagccc ccgcctacca ctcccatccc
     6121 ccagctgccg gcgccacagg cccacaagca ccctccccac ctctcggggc cctcaccctt
     6181 ctccatgaat gccaacctgc ctccccctcc agccctgaag cccctgagct ccctgtccac
     6241 acatcacccc ccgtcggctc accccccacc cctgcaactc atgcctcaga gccagccatt
     6301 gccctcctcg cccgcccagc cccccgggct gacccagagc cagaacctgc ccccgccccc
     6361 tgcctcccac ccccctacag gcctccacca ggtggccccc caacccccgt ttgctcagca
     6421 cccctttgtc cctggaggcc ctcctcccat cacccctccg acctgcccct ccacctctac
     6481 cccaccggcg ggacctggca cctcggccca gccaccctgc tctggtgcgg cggcttcagg
     6541 aggcagcata gcgggggggt cgtcctgccc actccccacc gtccagatca aggaggaggc
     6601 tctggacgac gctgaggagc ctgagagccc ccctccccca ccaaggagcc cgtccccgga
     6661 gcccactgtg gtggacaccc ccagtcacgc cagccagtca gctaggttct acaaacacct
     6721 ggaccggggc tacaactcgt gtgcccggac agacctgtac ttcatgcctc tggccgggtc
     6781 caagctggcc aagaagaggg aggaggccat tgagaaggcc aagcgcgagg ctgagcagaa
     6841 agcccgagag gagcgagagc gggagaagga gaaggagaag gagcgggagc gggagcgaga
     6901 gcgggagcgc gaggcagagc gggcggctaa ggcgtccagc tcagcgcatg aaggtcgcct
     6961 cagtgaccca cagctcagtg gtcctggcca catgcggcca tccttcgagc caccaccaac
     7021 caccattgct gctgtgcccc cctacatcgg gcccgacaca cctgcccttc ggactctgag
     7081 cgagtacgcc cggccccacg tcatgtcgcc caccaaccgc aaccacccct tctacatgcc
     7141 ccttaacccc acggaccccc tgctggccta ccacatgcct ggcctctaca acgtcgaccc
     7201 caccatccgc gagcgggagc tccgggagcg ggagatccga gagcgggaga tccgagagcg
     7261 ggagctgcgg gagaggatga agccgggctt cgaggtgaag cccccagagc tggaccccct
     7321 gcacccagcc gccaacccca tggagcactt tgcccggcac agcgccctca ccatcccccc
     7381 gaccgccggg ccccaccctt ttgcttcttt ccacccgggc ctgaacccct tggagaggga
     7441 gagactggcc ctggcgggcc cccagctgcg gcccgagatg agctaccctg acagactggc
     7501 agccgagcgt atccacgcag agcgcatggc atcgctgacc agcgatcccc tggcccgact
     7561 gcagatgttc aacgtgactc cgcaccatca ccagcactct cacattcact cccacctcca
     7621 cctccaccag caggaccccc tccaccaagg ttcagcaggc cccgttcacc cgctggtcga
     7681 ccccctgact gccggtcccc acctggctcg cttcccctac ccgcctggca ctctccccaa
     7741 ccctctgctt ggacagcccc cacacgagca cgagatgctt cgccacccag ttttcggcac
     7801 cccctacccc cgtgacctgc ctggggccat cccacccccc atgtcagcag cccaccagct
     7861 gcaggccatg catgcccagt cggccgagct gcagagactg gccatggagc agcagtggct
     7921 gcatggacac ccccacatgc atggtggcca cctaccaagt caggaagatt attacagtcg
     7981 actgaagaaa gaaggtgaca agcagttata gtaatgaact agtccgtaac ttgaaagtat
     8041 ttcgatttct tggctttata tatcttgtgg aaaggacgat caatctcgta tgtacagcag
     8101 ataacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8161 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8221 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8281 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8341 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8401 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8461 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     8521 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8581 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8641 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8701 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8761 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     8821 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8881 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8941 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9001 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     9061 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     9121 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9181 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     9241 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9301 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9361 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     9421 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     9481 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     9541 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     9601 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     9661 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9721 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     9781 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     9841 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     9901 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     9961 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    10021 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
    10081 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    10141 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    10201 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    10261 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    10321 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    10381 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    10441 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    10501 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    10561 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    10621 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    10681 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    10741 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    10801 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    10861 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    10921 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    10981 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    11041 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    11101 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    11161 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    11221 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    11281 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    11341 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    11401 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    11461 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    11521 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    11581 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    11641 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    11701 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    11761 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    11821 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    11881 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    11941 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    12001 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    12061 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    12121 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    12181 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    12241 aacaaaagct ggagctgcaa gc
//
