LOCUS       sequence-396178-       13957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13957)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1885
FEATURES             Location/Qualifiers
     source          1..13957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    9814..10402
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9867..9887)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10285..10296)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10474..10707
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10785..10906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10822..10841)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10876..10895
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10946..11081
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11101..11120)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11102..11120)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11130..11147)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11130..11146)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11139..11161)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11288..11743
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11375..11394)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11585..11606
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11769..11873
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11874..11942,11943..12734)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12092..12111)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12905..13493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13394..13413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13647..13664
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13781..13802
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13817..13822,13823..13840,13841..13847)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13855..13871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13860..13882
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13879..13895
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13879..13895
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13914..13934
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13916..13934
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggat ttatctgctc ttcgcgttga
     4141 agaagtacaa aatgtcatta atgctatgca gaaaatctta gagtgtccca tctgtctgga
     4201 gttgatcaag gaacctgtct ccacaaagtg tgaccacata ttttgcaaat tttgcatgct
     4261 gaaacttctc aaccagaaga aagggccttc acagtgtcct ttatgtaaga atgatataac
     4321 caaaaggagc ctacaagaaa gtacgagatt tagtcaactt gttgaagagc tattgaaaat
     4381 catttgtgct tttcagcttg acacaggttt ggagtatgca aacagctata attttgcaaa
     4441 aaaggaaaat aactctcctg aacatctaaa agatgaagtt tctatcatcc aaagtatggg
     4501 ctacagaaac cgtgccaaaa gacttctaca gagtgaaccc gaaaatcctt ccttgcagga
     4561 aaccagtctc agtgtccaac tctctaacct tggaactgtg agaactctga ggacaaagca
     4621 gcggatacaa cctcaaaaga cgtctgtcta cattgaattg ggatctgatt cttctgaaga
     4681 taccgttaat aaggcaactt attgcagtgt gggagatcaa gaattgttac aaatcacccc
     4741 tcaaggaacc agggatgaaa tcagtttgga ttctgcaaaa aaggctgctt gtgaattttc
     4801 tgagacggat gtaacaaata ctgaacatca tcaacccagt aataatgatt tgaacaccac
     4861 tgagaagcgt gcagctgaga ggcatccaga aaagtatcag ggtagttctg tttcaaactt
     4921 gcatgtggag ccatgtggca caaatactca tgccagctca ttacagcatg agaacagcag
     4981 tttattactc actaaagaca gaatgaatgt agaaaaggct gaattctgta ataaaagcaa
     5041 acagcctggc ttagcaagga gccaacataa cagatgggct ggaagtaagg aaacatgtaa
     5101 tgataggcgg actcccagca cagaaaaaaa ggtagatctg aatgctgatc ccctgtgtga
     5161 gagaaaagaa tggaataagc agaaactgcc atgctcagag aatcctagag atactgaaga
     5221 tgttccttgg ataacactaa atagcagcat tcagaaagtt aatgagtggt tttccagaag
     5281 tgatgaactg ttaggttctg atgactcaca tgatggggag tctgaatcaa atgccaaagt
     5341 agctgatgta ttggacgttc taaatgaggt agatgaatat tctggttctt cagagaaaat
     5401 agacttactg gccagtgatc ctcatgaggc tttaatatgt aaaagtgaaa gagttcactc
     5461 caaatcagta gagagtaata ttgaagacaa aatatttggg aaaacctatc ggaagaaggc
     5521 aagcctcccc aacttaagcc atgtaactga aaatctaatt ataggagcat ttgttactga
     5581 gccacagata atacaagagc gtcccctcac aaataaatta aagcgtaaaa ggagacctac
     5641 atcaggcctt catcctgagg attttatcaa gaaagcagat ttggcagttc aaaagactcc
     5701 tgaaatgata aatcagggaa ctaaccaaac ggagcagaat ggtcaagtga tgaatattac
     5761 taatagtggt catgagaata aaacaaaagg tgattctatt cagaatgaga aaaatcctaa
     5821 cccaatagaa tcactcgaaa aagaatctgc tttcaaaacg aaagctgaac ctataagcag
     5881 cagtataagc aatatggaac tcgaattaaa tatccacaat tcaaaagcac ctaaaaagaa
     5941 taggctgagg aggaagtctt ctaccaggca tattcatgcg cttgaactag tagtcagtag
     6001 aaatctaagc ccacctaatt gtactgaatt gcaaattgat agttgttcta gcagtgaaga
     6061 gataaagaaa aaaaagtaca accaaatgcc agtcaggcac agcagaaacc tacaactcat
     6121 ggaaggtaaa gaacctgcaa ctggagccaa gaagagtaac aagccaaatg aacagacaag
     6181 taaaagacat gacagcgata ctttcccaga gctgaagtta acaaatgcac ctggttcttt
     6241 tactaagtgt tcaaatacca gtgaacttaa agaatttgtc aatcctagcc ttccaagaga
     6301 agaaaaagaa gagaaactag aaacagttaa agtgtctaat aatgctgaag accccaaaga
     6361 tctcatgtta agtggagaaa gggttttgca aactgaaaga tctgtagaga gtagcagtat
     6421 ttcattggta cctggtactg attatggcac tcaggaaagt atctcgttac tggaagttag
     6481 cactctaggg aaggcaaaaa cagaaccaaa taaatgtgtg agtcagtgtg cagcatttga
     6541 aaaccccaag ggactaattc atggttgttc caaagataat agaaatgaca cagaaggctt
     6601 taagtatcca ttgggacatg aagttaacca cagtcgggaa acaagcatag aaatggaaga
     6661 aagtgaactt gatgctcagt atttgcagaa tacattcaag gtttcaaagc gccagtcatt
     6721 tgctccgttt tcaaatccag gaaatgcaga agaggaatgt gcaacattct ctgcccactc
     6781 tgggtcctta aagaaacaaa gtccaaaagt cacttttgaa tgtgaacaaa aggaagaaaa
     6841 tcaaggaaag aatgagtcta atatcaagcc tgtacagaca gttaatatca ctgcaggctt
     6901 tcctgtggtt ggtcagaaag ataagccagt tgataatgcc aaatgtagta tcaaaggagg
     6961 ctctaggttt tgtctatcat ctcagttcag aggcaacgaa actggactca ttactccaaa
     7021 taaacatgga cttttacaaa acccatatcg tataccacca ctttttccca tcaagtcatt
     7081 tgttaaaact aaatgtaaga aaaatctgct agaggaaaac tttgaggaac attcaatgtc
     7141 acctgaaaga gaaatgggaa atgagaacat tccaagtaca gtgagcacaa ttagccgtaa
     7201 taacattaga gaaaatgttt ttaaagaagc cagctcaagc aatattaatg aagtaggttc
     7261 cagtactaat gaagtgggct ccagtattaa tgaaataggt tccagtgatg aaaacattca
     7321 agcagaacta ggtagaaaca gagggccaaa attgaatgct atgcttagat taggggtttt
     7381 gcaacctgag gtctataaac aaagtcttcc tggaagtaat tgtaagcatc ctgaaataaa
     7441 aaagcaagaa tatgaagaag tagttcagac tgttaataca gatttctctc catatctgat
     7501 ttcagataac ttagaacagc ctatgggaag tagtcatgca tctcaggttt gttctgagac
     7561 acctgatgac ctgttagatg atggtgaaat aaaggaagat actagttttg ctgaaaatga
     7621 cattaaggaa agttctgctg tttttagcaa aagcgtccag aaaggagagc ttagcaggag
     7681 tcctagccct ttcacccata cacatttggc tcagggttac cgaagagggg ccaagaaatt
     7741 agagtcctca gaagagaact tatctagtga ggatgaagag cttccctgct tccaacactt
     7801 gttatttggt aaagtaaaca atataccttc tcagtctact aggcatagca ccgttgctac
     7861 cgagtgtctg tctaagaaca cagaggagaa tttattatca ttgaagaata gcttaaatga
     7921 ctgcagtaac caggtaatat tggcaaaggc atctcaggaa catcacctta gtgaggaaac
     7981 aaaatgttct gctagcttgt tttcttcaca gtgcagtgaa ttggaagact tgactgcaaa
     8041 tacaaacacc caggatcctt tcttgattgg ttcttccaaa caaatgaggc atcagtctga
     8101 aagccaggga gttggtctga gtgacaagga attggtttca gatgatgaag aaagaggaac
     8161 gggcttggaa gaaaataatc aagaagagca aagcatggat tcaaacttag gtgaagcagc
     8221 atctgggtgt gagagtgaaa caagcgtctc tgaagactgc tcagggctat cctctcagag
     8281 tgacatttta accactcagc agagggatac catgcaacat aacctgataa agctccagca
     8341 ggaaatggct gaactagaag ctgtgttaga acagcatggg agccagcctt ctaacagcta
     8401 cccttccatc ataagtgact cttctgccct tgaggacctg cgaaatccag aacaaagcac
     8461 atcagaaaaa gcagtattaa cttcacagaa aagtagtgaa taccctataa gccagaatcc
     8521 agaaggcctt tctgctgaca agtttgaggt gtctgcagat agttctacca gtaaaaataa
     8581 agaaccagga gtggaaaggt catccccttc taaatgccca tcattagatg ataggtggta
     8641 catgcacagt tgctctggga gtcttcagaa tagaaactac ccatctcaag aggagctcat
     8701 taaggttgtt gatgtggagg agcaacagct ggaagagtct gggccacacg atttgacgga
     8761 aacatcttac ttgccaaggc aagatctaga gggaacccct tacctggaat ctggaatcag
     8821 cctcttctct gatgaccctg aatctgatcc ttctgaagac agagccccag agtcagctcg
     8881 tgttggcaac ataccatctt caacctctgc attgaaagtt ccccaattga aagttgcaga
     8941 atctgcccag agtccagctg ctgctcatac tactgatact gctgggtata atgcaatgga
     9001 agaaagtgtg agcagggaga agccagaatt gacagcttca acagaaaggg tcaacaaaag
     9061 aatgtccatg gtggtgtctg gcctgacccc agaagaattt atgctcgtgt acaagtttgc
     9121 cagaaaacac cacatcactt taactaatct aattactgaa gagactactc atgttgttat
     9181 gaaaacagat gctgagtttg tgtgtgaacg gacactgaaa tattttctag gaattgcggg
     9241 aggaaaatgg gtagttagct atttctgggt gacccagtct attaaagaaa gaaaaatgct
     9301 gaatgagcat gattttgaag tcagaggaga tgtggtcaat ggaagaaacc accaaggtcc
     9361 aaagcgagca agagaatccc aggacagaaa gatcttcagg gggctagaaa tctgttgcta
     9421 tgggcccttc accaacatgc ccacagatca actggaatgg atggtacagc tgtgtggtgc
     9481 ttctgtggtg aaggagcttt catcattcac ccttggcaca ggtgtccacc caattgtggt
     9541 tgtgcagcca gatgcctgga cagaggacaa tggcttccat gcaattgggc agatgtgtga
     9601 ggcacctgtg gtgacccgag agtgggtgtt ggacagtgta gcactctacc agtgccagga
     9661 gctggacacc tacctgatac cccagatccc ccacagccac tactagtaat gaactagtcc
     9721 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgattcctc
     9781 agctggtgga agccattgac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     9841 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     9901 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     9961 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
    10021 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
    10081 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
    10141 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
    10201 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
    10261 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
    10321 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
    10381 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
    10441 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
    10501 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
    10561 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    10621 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    10681 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
    10741 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
    10801 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    10861 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
    10921 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    10981 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
    11041 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
    11101 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
    11161 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
    11221 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
    11281 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
    11341 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
    11401 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    11461 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    11521 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    11581 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    11641 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
    11701 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
    11761 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    11821 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    11881 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    11941 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    12001 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    12061 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    12121 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    12181 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    12241 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    12301 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    12361 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    12421 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    12481 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    12541 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    12601 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    12661 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    12721 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    12781 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    12841 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    12901 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    12961 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    13021 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    13081 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    13141 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    13201 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    13261 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    13321 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    13381 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    13441 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    13501 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    13561 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    13621 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    13681 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    13741 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    13801 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    13861 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    13921 accctcacta aagggaacaa aagctggagc tgcaagc
//
