LOCUS       sequence-396192-       12883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12883)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1958
FEATURES             Location/Qualifiers
     source          1..12883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8740..9328
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8793..8813)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9211..9222)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9400..9633
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9711..9832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9748..9767)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9802..9821
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9872..10007
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10027..10046)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10028..10046)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10056..10073)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10056..10072)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10065..10087)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10214..10669
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10301..10320)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10511..10532
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10695..10799
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10800..10868,10869..11660)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11018..11037)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11831..12419
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12320..12339
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12573..12590
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12707..12728
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12743..12748,12749..12766,12767..12773)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12781..12797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12786..12808
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12805..12821
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12805..12821
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12840..12860
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12842..12860
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgttg tgcgtagccg gagccaggtt
     4141 gaagagagaa ctcgatgcca ccgcaacggt attggcgaac cggcaggatg aaagtgagca
     4201 gtccagaaag cggcttatcg aacagagccg ggagttcaag aagaacactc cagaggattt
     4261 gcgcaagcag gtagcgccgc tgctgaagag tttccaagga gagattgatg cactgagtaa
     4321 aagaagcaag gaagctgaag cagctttctt gaatgtctac aaaagattga ttgacgtccc
     4381 agatcccgta ccagctttgg atctcggaca gcaactccag ctcaaagtgc agcgcctgca
     4441 cgatattgaa acagagaacc agaaacttag ggaaactctg gaagaataca acaaggaatt
     4501 tgctgaagtg aaaaatcaag aggttacgat aaaagcactt aaagagaaaa tccgagaata
     4561 tgaacagaca ctgaagaacc aagccgaaac catagctctt gagaaggaac agaagttaca
     4621 gaatgacttt gcagaaaagg agagaaagct gcaggagaca cagatgtcca ccacctcaaa
     4681 gctggaggaa gctgagcata aggttcagag cctacaaaca gccctggaaa aaactcgaac
     4741 agaattattt gacctgaaaa ccaaatacga tgaagaaact actgcaaagg ccgacgagat
     4801 tgaaatgatc atgacggacc ttgaaagggc aaaccagagg gcagaggtgg ctcagagaga
     4861 ggcggagacc ttaagggaac agctctcatc ggccaatcac tccctccagc tggcctcaca
     4921 gatccagaag gcaccagacg tggagcaggc catagaggtg ctgacccgct ccagcctaga
     4981 agttgagttg gccgccaagg agcgggagat cgcacagctg gtggaggacg tgcagagact
     5041 ccaggccagc ctcaccaagc tgcgggagaa ttcggccagc cagatctcac agcttgagca
     5101 gcagctgagc gccaaaaaca gcacactcaa acaactggaa gaaaaactca aaggccaggc
     5161 tgactatgaa gaggtgaaga aagagctgaa cattctgaag tccatggagt ttgcaccgtc
     5221 cgagggcgct gggacacagg atgcggccaa gcccctggag gtgctgttgc tggagaagaa
     5281 ccgctcgctg cagtccgaga acgccgcgct gcgcatctcc aacagcgacc tgagcgggtc
     5341 agccaggagg aaagggaaag accagcctga aagtcggcgc ccgggatctt tgccggcccc
     5401 ccctccttct cagttgcccc gcaacccggg ggagcaggct tccaatacta atggtacaca
     5461 ccagttctca ccagcggggt taagtcaaga ctttttcagc tcatccctgg caagccccag
     5521 cctacccctg gcttctacag gaaaatttgc actaaactct cttctccagc ggcagctaat
     5581 gcagtccttc tactccaagg ctatgcagga agccggaagc acaagcatga ttttttcaac
     5641 aggtccatac agcacaaact ccatatcttc ccaaagtcca ttacaacaaa gcccagatgt
     5701 caatggcatg gccccatccc ccagccagtc agaaagtgct gggagcgtct ccgagggcga
     5761 ggagatggac actgcagaaa tcgcccggca ggtcaaagag cagctgatta agcacaatat
     5821 cggacaacgt attttcggac attatgtgtt gggactgtct caagggtccg tgagcgagat
     5881 tctggcccgg cccaagccat ggaataaact gactgttcgt ggcaaggagc catttcacaa
     5941 gatgaaacag ttcctctccg atgagcagaa catcctggcc ctccgtagca tccaaggcag
     6001 acaaagagag aatccaggcc agagcctgaa cagactattt caggaagtac cgaaacgaag
     6061 aaatgggtct gaaggtaaca tcaccacccg gatccgagcc tcggagactg gctctgatga
     6121 agccatcaag tccatcctag agcaagccaa gagggagctc caagtgcaga aaactgcaga
     6181 gccggcccag ccttcctccg catccggcag cgggaactct gatgacgcca tccgctccat
     6241 cctgcagcaa gcccgccggg agatggaggc ccagcaggct gccctcgacc ctgccttaaa
     6301 gcaggcacca ctgtcccaga gtgacatcac catcctcacc cccaagcttc tgtccacctc
     6361 gcccatgccc accgtgtcca gctacccacc tctcgccatc tccctgaaga agccctccgc
     6421 agctcctgag gccggtgcct ctgctctgcc gaaccccccg gccctcaaaa aggaggccca
     6481 ggacgccccc gggctggacc cccagggagc agccgattgt gcacaagggg tcctgagaca
     6541 ggtgaaaaat gaggtgggcc gcagcggtgc ctggaaggac cactggtgga gcgcggtgca
     6601 gccggagaga agaaatgccg cctcctccga ggaggccaag gccgaagaaa cgggcggcgg
     6661 gaaagagaag ggcagcggtg gcagcggagg tggcagccag cctcgggccg agcgcagtca
     6721 gctccaggga ccctcgtcgt cagagtactg gaaggagtgg cccagcgctg agtccccata
     6781 ctcccagagc tcagagctga gtctgaccgg ggccagccgc agcgagacac cacagaacag
     6841 ccccctgcca tcctccccga tcgtgcccat gtccaagccc accaagccct cggtcccccc
     6901 gctgaccccc gagcagtacg aggtctacat gtaccaggag gtggacacca tcgagctcac
     6961 ccggcaggtt aaggaaaagc tggccaagaa cggcatctgc cagagaatct tcggggagaa
     7021 ggtgctgggc ctgtcccagg gcagcgtcag cgacatgctg tcccgaccga agccatggag
     7081 caagctgacg cagaaaggcc gagaaccctt catccggatg cagctctggc tgaacggcga
     7141 gctaggccag ggtgttctac ccgtccaggg ccagcagcaa gggccagtcc tccactccgt
     7201 gacatcgctc caggacccgc tgcagcaggg ctgtgtgagc tcagaaagca ctccaaagac
     7261 ctccgccagc tgcagccctg cccctgagtc cccgatgagt tccagtgagt cggtgaagag
     7321 cctgaccgag ctggtccagc agccctgtcc ccccatcgag gcgagcaagg acagcaagcc
     7381 accagagccc agtgacccgc cagcatccga ctcccagccc acaaccccgc tgcctctctc
     7441 cggacactcg gccctcagca tccaagaatt agtagccatg tccccggagc tggacaccta
     7501 cggcataacc aagcgggtga aggaggtgct gacggacaac aacctcggcc agcgcttatt
     7561 tggggagacc atcttagggc tcacccaagg ctctgtctct gacctccttg cccgccccaa
     7621 accctggcat aagctcagtc tgaaaggacg agagcccttc gtccggatgc agctgtggct
     7681 gaacgacccc aacaatgtgg agaagctgat ggacatgaaa cggatggaga agaaagccta
     7741 catgaagcgg cggcacagct cagtcagtga cagccagccc tgcgaaccgc cctctgtcgg
     7801 caccgagtac agccagggcg ccagccccca gccccagcac cagctgaaga aaccccgggt
     7861 ggtgctggct ccggaggaga aggaggcgct gaaacgagcg tatcagcaaa agccataccc
     7921 gtcaccaaaa accatcgaag acctcgccac ccagctcaac ctgaaaacca gcaccgtcat
     7981 caactggttc cacaactaca ggtctcggat ccgcagagaa ctgttcattg aggaaattca
     8041 ggccgggagt cagggccagg cgggcgccag cgactcaccc tcggcccgca gcggccgggc
     8101 ggcgcccagc tcggagggcg acagctgcga cggcgtggag gccactgagg gcccaggcag
     8161 cgccgacacc gaggagccca agtctcaggg agaggccgag cgggaggagg tgccgcggcc
     8221 ggcggagcag acggagccgc cgccctcggg gaccccgggc ccggacgacg cccgcgacga
     8281 cgaccacgag ggaggccccg tggaaggccc ggggcccctg cccagccccg cctccgcgac
     8341 cgccaccgcc gcgcccgcgg cccccgagga cgccgctacc tcagccgccg ccgcgccggg
     8401 ggagggcccc gcggccccga gctccgcgcc gccgcccagc aacagcagca gcagcagcgc
     8461 cccccgcagg cccagctcgc tgcagagcct tttcggcctc cccgaggccg cgggcgcccg
     8521 ggactcgcgc gacaaccccc tgcgcaagaa gaaggccgcg aacttgaaca gcatcatcca
     8581 ccgcctggag aaggccgcca gccgggagga acctatcgaa tgggagttct agtaatgaac
     8641 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     8701 ttagaggaac gatgtctact agtgacgcgt taagtcgaca atcaacctct ggattacaaa
     8761 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     8821 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8881 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8941 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     9001 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     9061 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     9121 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     9181 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     9241 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     9301 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     9361 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     9421 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     9481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9601 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     9661 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     9721 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9781 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9841 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9901 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9961 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
    10021 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
    10081 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    10141 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    10201 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    10261 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    10321 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    10381 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    10441 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    10501 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    10561 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    10621 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
    10681 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    10741 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    10801 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    10861 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    10921 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10981 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    11041 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    11101 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    11161 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    11221 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    11281 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    11341 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    11401 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    11461 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    11521 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    11581 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    11641 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    11701 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    11761 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    11821 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    11881 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    11941 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    12001 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    12061 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    12121 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    12181 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    12241 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    12301 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    12361 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    12421 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    12481 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    12541 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    12601 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    12661 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    12721 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    12781 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    12841 caattaaccc tcactaaagg gaacaaaagc tggagctgca agc
//
