LOCUS       sequence-396204-       10261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10261)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2519
FEATURES             Location/Qualifiers
     source          1..10261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6118..6706
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6171..6191)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6589..6600)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6778..7011
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7089..7210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7126..7145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7180..7199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7250..7385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7405..7424)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7406..7424)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7434..7451)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7434..7450)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7443..7465)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7592..8047
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7679..7698)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7889..7910
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8073..8177
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8178..8246,8247..9038)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8396..8415)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9209..9797
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9698..9717
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9951..9968
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10085..10106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10121..10126,10127..10144,10145..10151)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10159..10175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10164..10186
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10183..10199
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10183..10199
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10218..10238
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10220..10238
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtcg gtctccagcg gcgtccagat
     4141 tctgacaaag ccagagaccg tggaccggag gagaagtgcg gaaacgacca aagaggccgg
     4201 gcgaccactg gagatggctg tgtccgagcc cgaggccagc gccgcgaagg aatggaagca
     4261 actggatcct gctcagagca acctgtataa tgatgtgatg ctggagaact actgcaacca
     4321 agcctcaatg gggtgtcaag ctcccaaacc agacatgatc tccaagttgg aaaaaggaga
     4381 agcaccatgg ttggggaagg ggaaaagacc cagtcaaggt tgtccaagta aaatagcaag
     4441 acccaagcaa aaagaaactg atggaaaagt ccagaaagat gatgaccagc ttgaaaatat
     4501 ccagaaatct caaaacaaac tcctcaggga agttgcagtc aagaagaaaa ctcaagctaa
     4561 gaagaatggc agtgactgtg gttcactggg gaaaaaaaat aatttgcata aaaaacatgt
     4621 tccttcaaag aaaaggcttc ttaaatttga gtcatgtgga aaaattttga aacagaattt
     4681 agatttacct gatcactcaa gaaactgtgt aaaaaggaaa tctgatgcag ctaaagaaca
     4741 caagaagtca ttcaaccata gcttatctga tacaaggaaa ggcaaaaagc aaactggaaa
     4801 gaaacatgag aaattatcca gccatagctc atctgataag tgtaacaaaa ctggcaaaaa
     4861 acatgacaaa ttatgctgtc atagttcatc ccatattaaa caggacaaaa ttcaaactgg
     4921 agagaaacat gagaaatcac ccagccttag ctcatctact aagcatgaaa aacctcaagc
     4981 ttgtgtgaaa ccctatgaat gtaatcaatg tggaaaggtt ctcagccata aacaaggact
     5041 cattgaccat cagagagttc atactgggga gaaaccatat gaatgtaatg aatgtgggat
     5101 agcctttagc caaaagtcac accttgttgt acatcagaga actcacaccg gagaaaaacc
     5161 atatgaatgt attcagtgtg gcaaagccca tggtcataaa catgcactca ctgaccatct
     5221 aagaattcat actggagaaa agccctatga atgtgctgaa tgtgggaaaa ccttcagaca
     5281 cagctcaaac cttattcaac atgtgagatc tcacacaggt gagaagccat atgaatgtaa
     5341 ggaatgtggg aagtctttta ggtataactc atctcttacc gaacatgtga gaacacatac
     5401 aggtgaaata ccatatgaat gcaatgaatg tggaaaagcc tttaagtata gctcatccct
     5461 tactaaacac atgagaattc atacaggtga gaaacccttt gaatgtaatg aatgtgggaa
     5521 agctttcagc aagaagtcac acctcattat acatcaaaga actcatacta aggagaaacc
     5581 ttataaatgt aatgagtgtg gaaaagcctt tggacatagc tcatctctta cttaccatat
     5641 gagaactcat acaggtgaaa gtccctttga atgtaatcaa tgtgggaaag gctttaaaca
     5701 aattgaaggc cttactcaac atcagagagt tcatactgga gagaaaccgt atgagtgtaa
     5761 tgaatgtggg aaagccttta gccaaaagtc tcacctcatt gtacatcaga gaactcatac
     5821 tggggagaaa ccttatgaat gtaacgaatg tgaaaaagcc tttaatgcaa aatcacagct
     5881 tgttatacat cagcgatccc acactggaga aaaaccctat gaatgtaatg aatgtgggaa
     5941 aactttcaaa caaaatgcat ccctaaccaa acatgtgaaa actcattcag aagataaatc
     6001 tcatgagtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     6061 atcttgtgga aaggacgacc accacccggt actccaagat atacgcgtta agtcgacaat
     6121 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6181 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6241 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6301 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6361 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6421 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6481 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     6541 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6601 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6661 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     6721 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6781 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     6841 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6901 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6961 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     7021 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     7081 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7141 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7201 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     7261 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7321 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7381 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     7441 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     7501 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     7561 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     7621 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7681 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7741 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7801 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7861 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7921 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7981 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     8041 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8101 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8161 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8221 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8281 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8341 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8401 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     8461 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8521 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8581 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8641 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8701 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8761 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8821 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8881 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8941 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9001 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9061 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9121 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9181 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9241 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9301 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9361 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9421 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9481 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9541 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9601 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9661 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9721 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9781 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9841 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9901 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9961 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    10021 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    10081 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    10141 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    10201 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    10261 c
//
