LOCUS       sequence-396209-       12238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12238)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1202
FEATURES             Location/Qualifiers
     source          1..12238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8095..8683
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8148..8168)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8566..8577)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8755..8988
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9066..9187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9103..9122)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9157..9176
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9227..9362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9382..9401)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9383..9401)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9411..9428)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9411..9427)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9420..9442)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9569..10024
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9656..9675)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9866..9887
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10050..10154
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10155..10223,10224..11015)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10373..10392)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11186..11774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11675..11694
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11928..11945
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12062..12083
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12098..12103,12104..12121,12122..12128)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12136..12152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12141..12163
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12160..12176
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12160..12176
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12195..12215
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12197..12215
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtct tcccttcctg ggtgcattgg
     4141 tttggatgca gcaacagcta cagtggagtc tgaagagatt gcagagctgc aacaggcagt
     4201 ggttgaggaa ctgggtatct ctatggagga acttcggcat ttcatcgatg aggaactgga
     4261 gaagatggat tgtgtacagc aacgcaagaa gcagctagca gagttagaga catgggtaat
     4321 acagaaagaa tctgaggtgg ctcacgttga ccaactcttt gatgatgcat ccagggcagt
     4381 gactaattgt gagtctttgg tgaaggactt ctactccaag ctgggactac aataccggga
     4441 cagtagctct gaggacgaat cttcccggcc tacagaaata attgagattc ctgatgaaga
     4501 tgatgatgtc ctcagtattg attcaggtga tgctgggagc agaactccaa aagaccagaa
     4561 gctccgtgaa gctatggctg ccttaagaaa gtcagctcaa gatgttcaga agttcatgga
     4621 tgctgtcaac aagaagagca gttcccagga tctgcataaa ggaaccttga gtcagatgtc
     4681 tggagaacta agcaaagatg gtgacctgat agtcagcatg cgaattctgg gcaagaagag
     4741 aactaagact tggcacaaag gcacccttat tgccatccag acagttgggc cagggaagaa
     4801 atacaaggtg aaatttgaca acaaaggaaa gagtctactg tcggggaacc atattgccta
     4861 tgattaccac cctcctgctg acaagctgta tgtgggcagt cgggtggtcg ccaaatacaa
     4921 agatgggaat caggtctggc tctatgctgg cattgtagct gagacaccaa acgtcaaaaa
     4981 caagctcagg tttctcattt tctttgatga tggctatgct tcctatgtca cacagtcgga
     5041 actgtatccc atttgccggc cactgaaaaa gacttgggag gacatagaag acatctcctg
     5101 ccgtgacttc atagaggagt atgtcactgc ctaccccaac cgccccatgg tactgctcaa
     5161 gagtggccag cttatcaaga ctgagtggga aggcacgtgg tggaagtccc gagttgagga
     5221 ggtggatggc agcctagtca ggatcctctt cctggatgac aaaagatgtg agtggatcta
     5281 tcgaggctct acacggctgg agcccatgtt cagcatgaaa acatcctcag cctctgcact
     5341 ggagaagaag caaggacagc tcaggacacg tccaaatatg ggtgctgtga ggagcaaagg
     5401 ccctgttgtc cagtacacac aggatctgac cggtactgga acccagttca agccagtgga
     5461 acccccacag cctacagctc cacctgcccc acctttccca cctgctccac ctctatcccc
     5521 ccaagcaggt gacagtgact tggaaagcca gcttgcccag tcacggaagc aggtagccaa
     5581 aaagagcacg tcctttcgac caggatctgt gggctctggt cattcctccc ctacatctcc
     5641 tgcactcagt gaaaatgtct ctggtgggaa acctgggatc aaccagacat atagatcacc
     5701 tttaggctcc acagcctctg ccccagcacc ctcagcactc ccggcccctc cagcaccccc
     5761 agtcttccat ggcatgctgg agcgggcccc agcagagccc tcctaccgtg ctcccatgga
     5821 gaagcttttc tacttacctc atgtctgcag ctatacctgt ctgtctcgag tcagacctat
     5881 gaggaatgag cagtaccggg gcaagaaccc tctgctggtc ccgttactat atgacttccg
     5941 gcggatgaca gcccggcgtc gagttaaccg caagatgggc tttcatgtta tctataagac
     6001 accttgtggt ctctgccttc ggacaatgca ggagatagaa cgctaccttt tcgagactgg
     6061 ctgtgacttc ctcttcctgg agatgttctg tttggatcca tatgttcttg tggaccgaaa
     6121 gtttcagccc tataagcctt tttactatat tttggacatc acttatggga aggaagatgt
     6181 tcccctatcc tgtgtcaatg agattgacac aacccctcca ccccaggtgg cctacagcaa
     6241 ggaacgtatc ccgggcaagg gtgttttcat taacacaggc cctgaatttc tggttggctg
     6301 tgactgcaag gatgggtgtc gggacaagtc caagtgtgcc tgccatcaac taactatcca
     6361 ggctacagcc tgtaccccag gaggccaaat caaccctaac tctggctacc agtacaagag
     6421 actagaagag tgtctaccca caggggtata tgagtgtaac aaacgctgca aatgtgaccc
     6481 aaacatgtgc acaaaccggt tggtgcaaca tggactacaa gttcggctac agctattcaa
     6541 gacacagaac aagggctggg gtatccgctg cttggatgac attgccaaag gctcttttgt
     6601 ttgtatttat gcaggcaaaa tcctgacaga tgactttgca gacaaggagg gtctggaaat
     6661 gggtgatgag tactttgcaa atctggacca tatcgagagc gtggagaact tcaaagaagg
     6721 atatgagagt gatgccccct gttcctctga cagcagtggt gtagacttga aggaccagga
     6781 agatggcaac agcggtacag aggaccctga agagtccaat gatgatagct cagatgataa
     6841 cttctgtaag gatgaggact tcagcaccag ttcagtgtgg cggagctatg ctacccggag
     6901 gcagacccgg ggccagaaag agaacggact ctctgagaca acttccaagg actcccaccc
     6961 cccagatctt ggacccccac atattcctgt tcctccctca atccctgtag gtggctgcaa
     7021 tccaccttcc tccgaagaga cacccaagaa caaggtggcc tcatggttga gctgcaatag
     7081 tgtcagtgaa ggtggttttg ctgactctga tagccattca tccttcaaga ctaatgaagg
     7141 tggggagggc cgggctgggg gaagccgaat ggaggctgag aaggcctcca cctcaggact
     7201 aggcatcaag gatgagggag acatcaaaca ggccaagaaa gaggacactg acgaccgaaa
     7261 caagatgtca gtagttactg aaagctctcg aaattacggt tacaatcctt ctcctgtgaa
     7321 gcctgaagga cttcgccgcc cacctagtaa gactagtatg catcaaagcc gaagactcat
     7381 ggcttctgct cagtccaacc ctgatgatgt cctgacactg tccagcagca cagaaagtga
     7441 gggggaaagt gggaccagcc gaaagcccac tgctggtcag acttcggcta cagcggttga
     7501 cagtgatgat atccagacca tatcctctgg ctctgaaggg gatgactttg aggacaagaa
     7561 gaacatgact ggtccaatga agcgtcaagt ggcagtaaaa tcaacccgag gctttgctct
     7621 taaatcaacc catgggattg caattaaatc aaccaacatg gcctctgtgg acaaggggga
     7681 gagcgcacct gttcgtaaga acacacgcca attctatgat ggcgaggagt cttgctacat
     7741 cattgatgcc aagcttgaag gcaacctggg ccgctacctc aaccacagtt gcagccccaa
     7801 cctgtttgtc cagaatgtct tcgtggatac ccatgatctt cgcttcccct gggtggcctt
     7861 ctttgccagc aaaatccggg ctgggacaga acttacttgg gactacaact acgaggtggg
     7921 cagtgtggaa ggcaaggagc tactctgttg ctgtggggcc attgaatgca gaggacgtct
     7981 tctttagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     8041 ttgtggaaag gacgattatc aggtaaactg aggctgagca cgcgttaagt cgacaatcaa
     8101 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     8161 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8221 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8281 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8341 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8401 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8461 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8521 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8581 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8641 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     8701 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     8761 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     8821 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8881 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8941 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     9001 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     9061 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9181 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     9241 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     9301 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9361 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     9421 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     9481 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9541 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     9601 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9661 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9721 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9781 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9841 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9901 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9961 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    10021 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    10081 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10141 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    10201 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    10261 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    10321 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    10381 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    10441 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10501 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10561 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10621 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    10681 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    10741 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10801 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10861 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10921 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10981 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    11041 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    11101 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    11161 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    11221 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    11281 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    11341 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    11401 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    11461 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11521 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11581 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    11641 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    11701 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11761 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11821 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11881 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    11941 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    12001 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    12061 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    12121 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    12181 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
