LOCUS       sequence-396221-       11272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11272)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2865
FEATURES             Location/Qualifiers
     source          1..11272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4113..4122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7129..7717
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7182..7202)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7600..7611)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7789..8022
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8100..8221
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8137..8156)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8191..8210
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8261..8396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8416..8435)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8417..8435)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8445..8462)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8445..8461)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8454..8476)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8603..9058
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8690..8709)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8900..8921
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9084..9188
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9189..9257,9258..10049)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9407..9426)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10220..10808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10709..10728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10962..10979
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11096..11117
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11132..11137,11138..11155,11156..11162)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11170..11186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11175..11197
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11194..11210
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11194..11210
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11229..11249
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11231..11249
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggtg gccatggact tgggctctcc
     4141 ctcgctccct aagaagagcc tgcctgtccc tggggccctg gagcaggtgg ccagtcggct
     4201 gagcagcaaa gtggctgcag aggttcctca tggcagcaaa caggagctgc aggacctcaa
     4261 ggcccagagc ctgaccacct gcgaggtctg cggtgcctgc tttgagaccc gaaagggcct
     4321 gtccagccac gcgcgctccc acctgcggca gctgggagtg gcagagtcgg aaagcagcgg
     4381 cgcacccatc gacctcctct acgagcttgt gaagcagaag ggtctgcctg acgcccacct
     4441 tgggctgccc ccaggcctgg ctaagaagtc cagctcactg aaggaggtgg tcgccggggc
     4501 cccccggccc ggcttgctca gcctggccaa gcccttggat gcccctgctg tcaacaaagc
     4561 catcaagtcg cctcccggct tctcggccaa gggcctgggc cacccgccca gctctccact
     4621 cctcaaaaag acaccactgg ccctggcggg ctcccctacc cctaagaatc ctgaggacaa
     4681 gagcccccag ctgtccctga gcccccggcc ggcctcccca aaggcacagt ggcctcagtc
     4741 tgaggatgag gggcccttga acctcacttt agatagtgac gggggcagag agctggactg
     4801 ccagctgtgc ggtgcctggt ttgagacccg caagggcctg tctagccacg cccgtgccca
     4861 cctgcgccac ctgggcgtca gcgatccgga cgccaaggga tcccccatag acgtgctcca
     4921 cgggctcatc aggagggacg gcgtccagat ccgcctccca cccaggcgcg gcgccctggc
     4981 ccacccgggg cggccgcctc ccacctccgc ggccctctcc ttgcttcccc ccccaccgcc
     5041 ggccaagaag gccaagctga aggccgcggg tatggccagc ccctggggga agcaggacct
     5101 ctcggccgcc gcagccgccg gcattttctg ggcctctgat gtggagccgt ctcctctcaa
     5161 cctctcctca ggcccagagc cagcacgaga catccgctgc gagttctgtg gtgagttctt
     5221 cgagaaccgc aagggcctct cgagccacgc gcgctcccat ctgcggcaaa tgggcgtgac
     5281 cgagtggtac gtcaatggct cgcccatcga cacgctgcgg gagatcctga agagacggac
     5341 ccagtctcgg cctggtggac ctcccaaccc accagggcca agcccaaaag ccctggccaa
     5401 gatgatgggc ggcgcaggtc ctggcagctc actggaagcc cgcagcccct cggaccttca
     5461 catctcaccc ttggccaaga agttgccacc accaccgggc agccccctgg gccactcacc
     5521 aactgcctct cctcctccta cggcccgaaa gatgttccca ggcctggctg caccctcctt
     5581 gcccaagaag ctgaagcctg aacaaatacg ggtggagatc aagcgggaga tgctgccggg
     5641 ggcccttcat ggggaactgc acccatctga gggtccctgg ggggcaccac gggaagacat
     5701 gacacccctg aacctgtcgt cccgggcaga gccggtgcgc gacatccgct gtgagttctg
     5761 cggcgagttc ttcgagaacc gcaagggcct gtcgagtcac gcgcgctcac acctgcggca
     5821 gatgggtgtg accgagtggt ccgtcaatgg ttcgcccatc gacacactgc gagagatcct
     5881 caagaagaag tccaagccgt gcctcatcaa gaaggagcca ccggctggag acctggcccc
     5941 tgccctggct gaggacgggc ctcccaccgt ggcccctggg cccgtgcagt ccccactgcc
     6001 gctgtcgccc ctggctggcc ggccaggcaa accaggtgca gggccggccc aggttcctcg
     6061 tgagctcagc ctgacgccca tcactggggc caagccctca gccactggct acctgggctc
     6121 agtggcagcc aagcggcccc tgcaggagga ccgcctcctc ccagcagagg tcaaggccaa
     6181 gacctacatc cagactgaac tgcccttcaa ggcaaagacc cttcatgaga agacctccca
     6241 ctcctccacc gaggcctgct gcgagctgtg tggcctttac tttgaaaacc gcaaggccct
     6301 ggccagccac gcacgggcac acctgcggca gttcggcgtg accgagtggt gcgtcaatgg
     6361 ctcgcccatc gagacactga gcgagtggat caaacaccgg ccccagaagg tgggcgccta
     6421 ccgcagctac atccagggcg gccgcccctt caccaagaag ttccgcagtg ccggccatgg
     6481 ccgtgacagt gacaagcggc cgtccctggg gctggcaccc gggggcctgg ccgtggtcgg
     6541 ccgcagtgcc ggaggggagc cagggcccga ggctggccgg gcagccgacg gtggtgagcg
     6601 gcctctggca gccagcccgc caggcaccgt gaaggctgag gagcaccagc ggcagaacat
     6661 caacaaattt gaacgccgac aagcccgccc tccagatgcc tccgcagccc ggggaggcga
     6721 ggacaccaat gacctacagc agaagctgga ggaggtgcgg caacccccac cccgagtccg
     6781 gccagtcccc tccctggtgc cccggccccc ccagacatca cttgtcaagt tcgtgggcaa
     6841 catctacacc ctcaaatgca ggttctgtga ggtggaattc cagggccccc tctccatcca
     6901 ggaagagtgg gtgcggcact tacagcggca catcctggag atgaacttct ccaaagcgga
     6961 ccccccacct gaggagtccc aggccccgca ggcacagaca gcggcggcag aggctcccta
     7021 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     7081 aaaggacgat aaagatcatg tcgtgatatg cttacgcgtt aagtcgacaa tcaacctctg
     7141 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7201 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7261 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7321 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7381 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7441 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7501 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7561 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7621 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7681 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     7741 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7801 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7861 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7921 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7981 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     8041 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     8101 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8161 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8221 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     8281 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8341 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8401 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     8461 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8521 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8581 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     8641 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     8701 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     8761 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     8821 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     8881 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     8941 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9001 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     9061 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9121 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9181 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9241 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9301 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9361 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9421 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9481 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9541 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9601 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9661 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9721 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9781 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9841 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9901 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9961 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10021 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10081 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10141 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10201 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10261 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10321 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10381 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10441 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10501 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10561 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10621 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10681 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10741 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10801 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10861 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10921 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10981 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11041 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11101 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11161 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    11221 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gc
//
