LOCUS       sequence-396223-       13132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13132)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2679
FEATURES             Location/Qualifiers
     source          1..13132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8989..9577
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9042..9062)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9460..9471)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9649..9882
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9960..10081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9997..10016)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10051..10070
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10121..10256
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10276..10295)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10277..10295)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10305..10322)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10305..10321)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10314..10336)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10463..10918
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10550..10569)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10760..10781
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10944..11048
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11049..11117,11118..11909)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11267..11286)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12080..12668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12569..12588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12822..12839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12956..12977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12992..12997,12998..13015,13016..13022)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13030..13046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13035..13057
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13054..13070
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13054..13070
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13089..13109
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13091..13109
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgctg cctccaaagc acttgtctgc
     4141 caccaaacct aagaagtcct gggccccaaa tctgtatgag ctagacagtg acttgactaa
     4201 ggagccggat gtcatcatag gagaaggtcc aactgactct gagttttttc atcagaggtt
     4261 tcggaaccta atctatgtgg aatttgttgg gcctcggaag accctgatca aactccgaaa
     4321 cctctgcctc gattggttgc agccggagac ccgcaccaag gaggagatca tcgagctctt
     4381 ggtccttgag cagtacctga ccatcatccc tgaaaagctc aagccttggg tgcgagcaaa
     4441 aaagccggag aactgtgaga agctcgtcac tctgctggag aattacaagg agatgtacca
     4501 accagaagac gacaacaaca gtgacgtgac cagcgacgac gacatgaccc ggaacagaag
     4561 agagtcctca ccacctcact cagtccattc tttcagtgac cgggactggg accggagggg
     4621 cagaagcaga gacatggagc cacgagaccg ctggtcccac accaggaacc caagaagcag
     4681 gatgcctccg cgggatcttt cccttcctgt ggtggcgaaa acaagctttg aaatggacag
     4741 agaggacgac agggactcca gggcttatga gtcccgatct caggatgctg aatcatacca
     4801 aaatgtggtg gacctcgctg aggacaggaa acctcacaac acaatccagg acaacatgga
     4861 aaactacagg aagctgctct ccctcgtgca gcttgctgaa gacgatggcc actcccacat
     4921 gacgcagggc cactcatcaa gatccaagag aagtgcctac ccaagcacca gtcgaggtct
     4981 aaaaactatg cctgaagcca aaaaatcaac ccaccggcgg gggatttgtg aagatgaatc
     5041 ttcccacgga gtgataatgg aaaaattcat caaggatgtg tcacgcagtt ccaaatcggg
     5101 aagagcaagg gagtcaagcg accggtcaca gagattcccc agaatgtcag atgataactg
     5161 gaaggacatt tcattgaaca agagggagtc agtgatccag cagcgggttt atgaagggaa
     5221 tgcatttagg ggaggcttta ggtttaattc aacccttgtt tccagaaaga gagttcttga
     5281 aagaaagagg cgctatcatt ttgacacaga tgggaagggc tcgattcacg atcaaaaagg
     5341 ctgtcccagg aagaagccct ttgaatgtgg tagtgagatg agaaaagcca tgagcgtgag
     5401 cagcctgagc agcctcagct ccccctcctt taccgagtca cagccaattg attttggggc
     5461 aatgccatat gtatgtgatg agtgtgggag gtcgttcagt gtcatctcag aatttgttga
     5521 gcaccagatc atgcatacta gagagaacct ctatgagtat ggtgagtcct ttatccacag
     5581 tgtggctgtc agtgaagttc agaaaagtca ggttggaggg aaacgttttg aatgtaagga
     5641 ctgtggagag accttcaata agagtgccgc cttggctgaa catcggaaga ttcatgctag
     5701 aggttatctt gtggaatgta agaatcagga atgtgaggaa gccttcatgc ctagccccac
     5761 ctttagtgag cttcagaaaa tatatggcaa agacaaattc tacgagtgca gggtgtgtaa
     5821 ggaaaccttc cttcatagtt ctgccctgat tgagcaccag aaaatccact ttggggatga
     5881 caaagataat gagcgtgaac atgaacgtga acgtgaacgt gagcgcgggg aaacctttag
     5941 gcccagccca gcccttaatg agtttcagaa aatgtatggt aaagagaaaa tgtacgaatg
     6001 taaggtgtgt ggggagactt tccttcatag ctcatccctg aaagaacatc agaaaatcca
     6061 tactagaggg aacccatttg aaaacaaggg taaagtgtgt gaggaaacct ttattcctgg
     6121 tcagtccctt aaaaggcgtc agaaaactta caataaggag aagctctgtg actttacaga
     6181 tggccgggat gccttcatgc aaagctcaga gctcagtgag catcagaaaa ttcattctcg
     6241 aaagaacctc tttgaaggca gagggtatga gaaatctgtc attcatagtg ggccattcac
     6301 tgaatctcag aagagtcata ctataacaag acctcttgaa agtgatgagg acgaaaaggc
     6361 gttcaccatt agctctaacc cctatgaaaa ccagaagatt cccactaagg aaaatgtcta
     6421 tgaggcaaaa tcatatgaga ggtctgttat tcatagctta gcctctgtgg aagctcagaa
     6481 aagtcacagt gtagcagggc ccagtaaacc aaaagtaatg gcagagtcta ccattcagag
     6541 cttcgatgct atcaaccatc agagagttcg tgctggaggg aacacctctg aaggaaggga
     6601 atacagtagg tctgttatcc atagcttagt ggcttccaaa cctccaagaa gtcacaatgg
     6661 aaatgaattg gtggaatcta atgagaaggg agaatcctcc atttatatct cagaccttaa
     6721 tgataagcga cagaagattc ctgccagaga gaacccttgt gaagggggca gtaagaatcg
     6781 caactatgaa gactctgtca tacagagtgt attccgtgcc aaacctcaga aaagtgttcc
     6841 tggagaggga tctggtgagt ttaagaagga tggcgaattc tctgttccca gctcaaatgt
     6901 ccgtgaatac cagaaggctc gtgctaaaaa gaaatacatt gagcatagga gcaatgagac
     6961 ctctgtaatt cactctctgc cttttggtga acaaacattt cgccctcgag ggatgctcta
     7021 tgaatgtcag gagtgtgggg agtgctttgc tcatagctct gacctcactg agcaccagaa
     7081 gattcatgat agggagaagc cctctggaag cagaaactat gaatggtctg tcattcgcag
     7141 cttggcccct actgaccctc aaacaagtta cgcccaagag cagtatgcta aagagcaagc
     7201 gcggaacaaa tgtaaggact tcagacaatt ttttgctacc agcgaagacc tcaacacaaa
     7261 ccagaaaatc tatgaccaag agaagtctca tggcgaggag tctcaaggcg agaatactga
     7321 tggggaggag acccacagcg aggagaccca tggtcaggag acaattgaag accctgtcat
     7381 tcaaggctca gacatggaag accctcagaa ggatgaccct gatgacaaaa tctatgaatg
     7441 tgaggactgt ggcctgggct ttgtggatct cacagacctc acagaccatc agaaagtcca
     7501 cagcaggaag tgcctggttg acagtcggga gtacacacat tctgtaattc acacccattc
     7561 catcagcgag tatcagagag attacactgg agagcagctg tatgaatgtc caaagtgtgg
     7621 ggaatctttt attcatagct cattcctttt cgagcatcag agaatccatg aacaagacca
     7681 gttgtattcc atgaaggggt gtgatgatgg ttttattgcc ctcttgccca tgaagccacg
     7741 gaggaatcgt gctgcagaga ggaatcctgc tcttgctggg tcggccattc gatgcctttt
     7801 gtgtggacaa ggcttcattc atagctctgc ccttaatgag catatgagac ttcataggga
     7861 agatgattta ctggagcaga gccagatggc tgaggaagct atcattccag gcttagccct
     7921 cactgagttt cagagaagtc agaccgaaga gagactcttt gaatgtgcag tctgtggaga
     7981 atctttcgtc aacccagcag aacttgcaga tcacgtaact gttcataaga atgagcccta
     8041 tgagtacggg tcctcctata ctcacacctc atttcttact gagcccctca aaggagctat
     8101 accattctat gaatgcaagg attgtggtaa gtcctttatt catagcacag tcctcactaa
     8161 acataaggag cttcatctgg aagaagaaga agaagatgaa gcagcagcag ctgcagcagc
     8221 agcagcccag gaagttgaag ccaatgtcca tgttccacaa gtagttctga ggattcaggg
     8281 cttaaacgta gaggctgctg agccagaagt ggaggctgcc gagccagaag tggaggctgc
     8341 tgagccagaa gtggaggctg ctgagccaaa cggagaggct gaagggccag atggagaggc
     8401 tgcagagccc attggagagg ctggacagcc aaatggagag gccgagcagc caaatgggga
     8461 tgctgatgag ccagatggtg caggtattga agacccagaa gaaagagctg aagagccaga
     8521 gggaaaagct gaagagccag agggagatgc cgacgagcct gacggtgtgg gaattgaaga
     8581 cccagaagaa ggtgaagatc aagagattca ggtagaagaa ccatactatg actgccatga
     8641 atgcacagaa accttcactt ccagcacagc attcagtgaa cacctgaaaa ctcatgccag
     8701 catgatcata tttgagcctg caaatgcctt tggggagtgc tcaggctaca tcgaacgtgc
     8761 cagcaccagc acaggtggtg ccaatcaagc tgatgagaag tacttcaaat gtgacgtctg
     8821 tgggcagctc ttcaatgacc gcctgtccct cgccagacac cagaataccc acactggcta
     8881 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     8941 aaaggacgac tggtggacgt gtgcggttat ggtacgcgtt aagtcgacaa tcaacctctg
     9001 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     9061 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     9121 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     9181 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     9241 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     9301 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     9361 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     9421 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     9481 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     9541 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     9601 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     9661 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     9721 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9781 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9841 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     9901 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     9961 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10021 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10081 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
    10141 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    10201 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    10261 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
    10321 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
    10381 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
    10441 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    10501 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    10561 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    10621 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    10681 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    10741 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    10801 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    10861 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
    10921 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    10981 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    11041 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    11101 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    11161 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    11221 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    11281 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    11341 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    11401 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    11461 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    11521 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    11581 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    11641 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    11701 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    11761 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    11821 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    11881 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    11941 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    12001 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    12061 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    12121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    12181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    12241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    12301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    12361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    12421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    12481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    12541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    12601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    12661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    12721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    12781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    12841 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    12901 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    12961 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    13021 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    13081 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gc
//
