LOCUS       sequence-396227-       13039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13039)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2778
FEATURES             Location/Qualifiers
     source          1..13039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    5114..5125
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     misc_feature    8896..9484
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8949..8969)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9367..9378)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9556..9789
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9867..9988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9904..9923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9958..9977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10028..10163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10183..10202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10184..10202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10212..10229)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10212..10228)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10221..10243)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10370..10825
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10457..10476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10667..10688
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10851..10955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10956..11024,11025..11816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11174..11193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11987..12575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12476..12495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12729..12746
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12863..12884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12899..12904,12905..12922,12923..12929)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12937..12953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12942..12964
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12961..12977
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12961..12977
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12996..13016
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12998..13016
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag ccggggtccg acgatttcct
     4141 accgccaccg gagtgcccgg tgttcgagcc tagctgggcc gagttccgag accctcttgg
     4201 ctacatcgcg aaaatcaggc ccatcgcaga gaaatcgggc atttgcaaga tccgcccacc
     4261 cgcggactgg cagccaccct ttgctgtgga agtggacaac ttcaggttta ccccccgaat
     4321 ccagaggctg aatgagctag aggcccagac gagagtgaaa ctgaactact tggaccagat
     4381 tgccaaattc tgggaaatcc agggctcctc cttaaagatt cccaatgtag aacggcggat
     4441 cttggacctc tacagtctca gcaaaattgt ggtggaggaa ggtggttatg aagctatctg
     4501 caaggaccgt cggtgggctc gggtagccca gcgcctcaac tatccaccag gcaaaaatat
     4561 tggctccttg ctacgctccc actacgaacg cattgtttat ccctatgaaa tgtaccagtc
     4621 tggagccaac cttgtgcagt gtaacacacg tccatttgat aatgaggaga aggacaagga
     4681 atacaaaccc cacagcatcc ccctacgaca gtctgtgcag ccttccaagt tcaacagcta
     4741 tggccggcgg gccaagagac tgcagcctga tccggaaccc acagaggaag acattgagaa
     4801 gaatccagag ctgaaaaagc tacagatcta tggggcaggc cccaagatga tgggcctggg
     4861 cctcatggcc aaagacaaga ctctgcggaa gaaagataag gaggggcctg agtgtccccc
     4921 cacagtagtg gtgaaggagg agttaggtgg ggatgtgaag gtggagtcaa catcgcctaa
     4981 gaccttcctg gagagcaagg aggagctgag tcacagccca gaaccctgca ccaagatgac
     5041 catgaggcta cggaggaacc acagcaatgc ccagtttatt gagtcatatg tctgccggat
     5101 gtgttctcga ggggatgagg atgacaagct cctgctgtgt gatggctgtg atgacaacta
     5161 ccacatcttc tgcctgctgc ctcctctgcc tgagatcccc aagggtgtct ggcggtgccc
     5221 aaagtgtgtc atggcggagt gtaagcggcc cccagaagcc tttggctttg agcaggctac
     5281 ccgggaatac actctgcaga gctttggcga gatggccgac tcctttaaag ctgactactt
     5341 caacatgccc gtgcatatgg tgcccacaga acttgtggag aaggagttct ggaggctggt
     5401 aaatagcatt gaggaagatg tgactgttga gtatggagct gacatccatt ccaaagaatt
     5461 tggcagcggt ttccctgtca gtgacagtaa acggcaccta acccccgaag aggaggagta
     5521 tgctaccagt ggttggaacc taaatgtgat gccggtgttg gaacagtctg tactgtgcca
     5581 catcaatgca gatatctctg gcatgaaggt gccctggctc tacgtgggca tggtcttctc
     5641 agccttttgc tggcatattg aggatcactg gagttactcc attaactacc tccactgggg
     5701 tgagccgaag acctggtatg gggtgccctc acttgcagca gaacatttgg aagaagtgat
     5761 gaagaagctg acacctgaac tatttgatag ccagcctgac ctcctgcacc aacttgtcac
     5821 cctcatgaat cccaacaccc tcatgtccca tggtgtgcca gttgtccgca caaaccagtg
     5881 tgcaggagag tttgtcatca ccttcccccg tgcttaccac agcggcttca accaaggcta
     5941 caactttgcc gaggctgtca acttttgcac tgctgactgg ttgcctgctg ggcgccagtg
     6001 cattgagcac taccgccggc tccggagata ctgcgtcttc tcccatgagg agcttatctg
     6061 caagatggct gcctgcccag agaagctaga cctgaacctg gcggcagctg tgcataagga
     6121 gatgttcatc atggtgcaag aagagcggcg tctacgaaag gccctgctgg agaagggtat
     6181 cacagaggct gagcgagagg ctttcgagct gctcccagat gatgagcgcc agtgtatcaa
     6241 gtgcaagact acgtgtttcc tgtcagccct ggcctgctac gactgcccag acggccttgt
     6301 ctgcctttcc cacatcaatg atctctgcaa gtgctccagt agccggcagt acctgcggta
     6361 tcggtatacc ttggatgagc ttcctgccat gctgcataag ctgaaggttc gggctgagtc
     6421 ctttgacacc tgggccaaca aagtgcgagt ggccctggag gtggaggatg ggcggaagcg
     6481 cagccttgaa gaactgaggg cactagagtc tgaagcccgt gagcggaggt ttcctaatag
     6541 tgagctgctg cagcaactaa agaactgcct gagtgaggca gaggcttgcg tgtcccgagc
     6601 tctgggactg gtcagcggcc aggaagctgg cccccacagg gtggctggtc tacagatgac
     6661 cctgactgag ctccgggcct ttctggacca gatgaacaac ctgccttgcg ccatgcacca
     6721 gattggggat gtcaagggtg ttctggaaca ggtggaggcc taccaggctg aggctcgtga
     6781 ggccctggcc tcactgccct ccagtccagg gctactgcag tccctgttgg agagggggcg
     6841 gcagctgggg gtggaggtgc ctgaggccca gcagctccag cggcaggtgg aacaggcgcg
     6901 atggctggat gaggtgaaac gcacactggc cccctcagcc cgaaggggca ccttggctgt
     6961 catgcgagga ctgttggtcg cgggtgccag tgtagcccct agccctgctg tggataaagc
     7021 ccaggccgag ctgcaggaac tgctgaccat tgctgaacgc tgggaggaga aagcccacct
     7081 ctgcctggag gccaggcaga agcatccacc agccacactt gaggccataa tccgtgaagc
     7141 ggaaaacatc cctgttcacc tgcccaacat ccaggctctc aaggaggctc ttgctaaggc
     7201 ccgggcctgg attgctgatg ttgatgagat ccaaaatggt gaccactacc cctgcctgga
     7261 tgacttggag ggcctagtag ctgtgggccg ggacctacct gtggggctgg aggagctgag
     7321 acagctagag ctacaggtac tgacagcgca ctcctggagg gagaaggcct ccaagacctt
     7381 cctcaagaaa aattcttgct acacgctgct ggaggttctc tgcccatgtg cagatgccgg
     7441 ctcagacagc accaagcgca gccggtggat ggagaaggag ctggggttgt acaaatctga
     7501 cacagagctg ctggggctgt ctgcgcagga cctcagggac ccaggctctg tgatcgtggc
     7561 cttcaaggag ggggaacaga aggagaagga gggtatcctg cagctgcgtc gcaccaattc
     7621 ggccaagccc agtccactgg catcatcgag cacggcctcc tctacaacct ctatctgtgt
     7681 gtgtgggcag gtgctggctg gggcgggagc tctgcagtgt gacctgtgtc aggactggtt
     7741 ccatgggcgg tgtgtgtcag tgcctcgcct cctcagctct ccgaggccca atcccacctc
     7801 atccccactg ctggcctggt gggaatggga caccaaattc ctgtgtccac tgtgtatgcg
     7861 ctcaaggcgc ccgcgcctgg agaccatcct ggcactgctg gtagccctgc agagactgcc
     7921 tgtgcggctg cccgagggcg aggccctgca gtgcctcaca gagagggcca tcagctggca
     7981 aggccgcgcc aggcaggctc tggcctctga agatgtgact gctcttttgg gacggctggc
     8041 tgagctccgc caacggctac aggctgaacc tagacctgag gagcctccta actaccctgc
     8101 agcccctgct tctgaccccc tcagagaggg cagtggcaag gatatgccta aggtccaggg
     8161 cttactggag aatggagaca gtgtgaccag tcctgagaag gtagccccgg aggagggctc
     8221 agatctggag ctgctgtcct cgctgttgcc acagttgact ggccctgtgt tggaactgcc
     8281 tgaggcaacc cgggccccct tggaggagct catgatggag ggggacctgc tcgaggtgac
     8341 cctggatgag aaccacagca tatggcagct gctgcaggct ggacagcccc cagacctgga
     8401 gaggatccgc acacttctgg agctggagaa ggcagagcgt cacgggagtc gggctcgggg
     8461 ccgggccctg gagaggcggc ggcggcggaa ggtggatcgg ggtggggagg gcgatgaccc
     8521 agcccgagag gagctagagc caaagagggt acggagctca gggccagagg ctgaggaggt
     8581 ccaggaggag gaagagctgg aggaggagac tgggggtgag ggcccccctg cacccatccc
     8641 caccactggc agccccagca cccaggagaa ccagaatggc ttggaaccgg cggaagggac
     8701 cacttcaggc ccctcggccc ctttctccac tctgactccc cggctgcatc tgccctgccc
     8761 acagcagccg cctcagcaac agttgtagta atgaactagt ccgtaacttg aaagtatttc
     8821 gatttcttgg ctttatatat cttgtggaaa ggacgatacc ggctaattcc tcaagtcgtt
     8881 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     8941 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     9001 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     9061 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     9121 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     9181 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     9241 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     9301 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     9361 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     9421 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     9481 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     9541 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     9601 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9661 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9721 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9781 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     9841 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     9901 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9961 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
    10021 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10081 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    10141 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
    10201 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
    10261 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
    10321 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
    10381 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    10441 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    10501 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    10561 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    10621 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    10681 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    10741 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    10801 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
    10861 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    10921 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    10981 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    11041 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    11101 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    11161 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    11221 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    11281 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    11341 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    11401 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    11461 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    11521 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    11581 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    11641 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    11701 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    11761 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    11821 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    11881 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    11941 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    12001 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    12061 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    12121 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    12181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    12241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    12301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    12361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    12421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    12481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    12541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    12601 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    12661 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    12721 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    12781 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    12841 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    12901 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    12961 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    13021 aaaagctgga gctgcaagc
//
