LOCUS       sequence-39623-e         584 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1142
FEATURES             Location/Qualifiers
     source          1..584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgcccttgct caccatggtg gcggaagttc ctataccttt tgaagaatag gaacttcgga
       61 ataggaactt cggcgcgccc gccctgtgaa aggacaagcc agagacccct ggagctcgga
      121 tgtggaagga tgtggggagc tgcctggtcc tgtctcccac tgccgcagag ggtaagccct
      181 ctcatactgc gcccgaaccc gctgcctcca ggtgcccctg ccagtcagct gcagtcaggg
      241 caggggcggc gctgcagctg actgtgagct tccactcgga gggagctgcc tcacctccct
      301 cctcccacgc cctctccccc ccacctgctc ctctgcctgg ctgacaccag gaatgactaa
      361 cgggagtttg gtccctggca gaaagcaggt acggaaccag gactcactga gggactctgg
      421 gcaaaaatgc ttttcttttc tgggcctcag tttcctcatt gatacggtga ggcagctgga
      481 ccccaaatga cctctagggg ccatgccagc tctcacccat gattctatga gaaggaaata
      541 aacagattta aaatggctcc cccagagtca aaatggaatt actg
//
