LOCUS       sequence-396238-       13150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13150)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1317
FEATURES             Location/Qualifiers
     source          1..13150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6796..6807
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             7039..7050
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    9007..9595
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9060..9080)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9478..9489)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9667..9900
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9978..10099
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10015..10034)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10069..10088
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10139..10274
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10294..10313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10295..10313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10323..10340)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10323..10339)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10332..10354)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10481..10936
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10568..10587)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10778..10799
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10962..11066
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11067..11135,11136..11927)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11285..11304)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12098..12686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12587..12606
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12840..12857
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12974..12995
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13010..13015,13016..13033,13034..13040)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13048..13064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13053..13075
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13072..13088
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13072..13088
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13107..13127
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13109..13127
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaag ttatatgtat ttctggttaa
     4141 cactggaact actctaacat ttgacactga acttacagtg caaactgtgg cagaccttaa
     4201 gcatgccatt caaagcaaat acaagattgc tattcaacac caggtgctgg tggtcaatgg
     4261 aggagaatgc atggctgcag atcgaagagt gtgtacctac agtgctggga cggatacaaa
     4321 tccaattttt ctttttaaca aagaaatgat cttatgtgat cgtccacctg ctattcctaa
     4381 aactaccttt tcgacagaaa atgacatgga aataaaagtt gaagaatctc ttatgatgcc
     4441 tgcagttttt catactgttg cttcaaggac acagcttgca ttggaaatgt atgaagttgc
     4501 caagaaactt tgttcttttt gtgaaggtct tgtacatgat gaacatcttc aacaccaagg
     4561 ctgggctgca atcatggcca acctggagga ctgttcaaat tcataccaaa agctactttt
     4621 caagtttgaa agtatttatt caaattatct gcagtccata gaagacatca agttaaaact
     4681 tactcattta ggaactgcag tttcagtaat ggccaagatt ccactgttgg agtgcctaac
     4741 cagacatagt tacagagaat gtttgggaag actggattct ttacctgaac atgaagactc
     4801 agaaaaagct gagatgaaaa gatccactga actggtgctc tctcctgata tgcctagaac
     4861 aactaacgaa tctttgttaa cctcatttcc caagtcagtg gaacatgtgt ccccagatac
     4921 cgcagatgct gaaagtggca aagaaattag ggaatcttgt caaagtactg ttcatcagca
     4981 agatgaaact acgattgaca ctaaagatgg tgatctgccc ttttttaatg tctctttgtt
     5041 agactggata aatgttcaag atagacctaa tgatgtggaa tctttggtca ggaagtgctt
     5101 tgattctatg agcaggcttg atccaaggat tattcgacca tttatagcag aatgccgtca
     5161 aactattgcc aaacttgata atcagaatat gaaagccatt aaaggacttg aagatcggct
     5221 ctacgccctg gaccagatga ttgctagctg tggccgactg gtgaatgaac agaaagagct
     5281 tgctcaggga tttttagcta atcagaagag agctgaaaac ttaaaggatg catctgtatt
     5341 acctgattta tgcctgagtc acgcaaatca gttgatgatt atgttgcaaa atcatagaaa
     5401 actgttagat attaagcaga agtgtaccac tgccaaacaa gaactagcaa ataacctaca
     5461 tgtcagactg aagtggtgtt gctttgtaat gcttcatgct gatcaagatg gagagaagtt
     5521 acaagctttg ctccgcctcg taatagagct gttagaaaga gtcaaaattg ttgaagctct
     5581 tagtacagtt cctcagatgt actgcttagc tgttgttgag gttgtaagaa gaaaaatgtt
     5641 cataaaacac tacagggagt gggctggtgc tttagtcaaa gatggaaaga gattatatga
     5701 agcagaaaaa tcaaaaaggg aatcctttgg gaaattattt aggaagtctt ttttaagaaa
     5761 tcgtctgttt aggggactgg actcctggcc cccttccttt tgtactcaaa agcctcgaaa
     5821 gtttgactgt gaacttccag atatttcatt aaaagattta cagtttctgc aatcattttg
     5881 tccttcggaa gttcagccat tcctcagggt tcccttactt tgtgactttg aacctctaca
     5941 ccagcatgta cttgctctac ataatttggt aaaagcagca caaagtttgg atgaaatgtc
     6001 acagaccatt acagatctac tgagtgaaca aaaggcatct gtgagtcaga catccccaca
     6061 gtctgcttct tcaccaagga tggaaagtac agcaggaatt acaactacta cctcaccgag
     6121 aactcctcca ccactgactg ttcaggatcc cttatgtcct gcagtttgtc ccttagaaga
     6181 attatctcca gatagtattg atgcacatac gtttgatttt gaaactattc cccatccaaa
     6241 catagaacag actattcacc aagtttcttt agacttggat tcattagcag aaagtcctga
     6301 atcagatttt atgtctgctg tgaatgagtt tgtaatagaa gaaaatttgt cgtctcctaa
     6361 tcctataagt gatccacaaa gcccagaaat gatggtggaa tcactttatt catcagttat
     6421 caatgcgata gacagtagac gaatgcagga tacaaatgta tgtggtaagg aggattttgg
     6481 agatcatact tctctgaatg tccagttgga aagatgtaga gttgttgccc aagactctca
     6541 cttcagtata caaaccatta aggaagacct ttgccacttt agaacatttg tacaaaaaga
     6601 acagtgtgac ttctcaaatt cattaaaatg tacagcagta gaaataagaa acattattga
     6661 aaaagtaaaa tgttctctgg aaataacact aaaagaaaaa catcaaaaag aactactgtc
     6721 tttaaaaaat gaatatgaag gtaaacttga cggactaata aaggaaactg aagagaatga
     6781 aaacaaaatt aaaaaattga agggagagtt agtatgcctt gaggaggttt tacaaaataa
     6841 agataatgaa tttgctttgg ttaaacatga aaaagaagct gtaatctgcc tgcagaatga
     6901 aaaggatcag aagttgttag agatggaaaa tataatgcac tctcaaaatt gtgaaattaa
     6961 agaactgaag cagtcacgag aaatagtgtt agaagactta aaaaagctcc atgttgaaaa
     7021 tgatgagaag ttacagttat tgagggcaga acttcagtcc ttggagcaaa gtcatctaaa
     7081 ggaattagag gacacacttc aggttaggca catacaagag tttgagaagg ttatgacaga
     7141 ccacagagtt tctttggagg aattaaaaaa ggaaaaccaa caaataatta atcaaataca
     7201 agaatctcat gctgaaatta tccaggaaaa agaaaaacag ttacaggaat taaaactcaa
     7261 ggtttctgat ttgtcagaca cgagatgcaa gttagaggtt gaacttgcgt tgaaggaagc
     7321 agaaactgat gaaataaaaa ttttgctgga agaaagcaga gcccagcaga aggagacctt
     7381 gaaatctctt cttgaacaag agacagaaaa tttgagaaca gaaattagta aactcaacca
     7441 aaagattcag gataataatg aaaattatca ggtgggctta gcagagctaa gaactttaat
     7501 gacaattgaa aaagatcagt gtatttccga gttaattagt agacatgaag aagaatctaa
     7561 tatacttaaa gctgaattaa acaaagtaac atctttgcat aaccaagcat ttgaaataga
     7621 aaaaaaccta aaagaacaaa taattgaact gcagagtaaa ttggattcag aattgagtgc
     7681 tcttgaaaga caaaaagatg aaaaaattac ccaacaagaa gagaaatacg aagctattat
     7741 ccagaacctt gagaaagaca gacaaaaatt ggtcagcagc caggagcaag acagagaaca
     7801 gttaattcag aagcttaatt gtgaaaaaga tgaagctatt cagactgccc taaaagaatt
     7861 taaattggag agagaagttg ttgagaaaga gttattagaa aaagttaaac atcttgagaa
     7921 tcaaatagca aaaagtcctg ccattgactc taccagagga gattcttcaa gcttagttgc
     7981 tgaacttcaa gaaaagcttc aggaagaaaa agctaagttt ctagaacaac ttgaagagca
     8041 agaaaaaaga aagaatgaag aaatgcaaaa tgttcgaaca tctttgattg cggaacaaca
     8101 gaccaatttt aacactgttt taacaagaga gaaaatgaga aaagaaaaca taataaatga
     8161 tcttagtgat aagttgaaaa gtacaatgca gcaacaagaa cgggataaag atttgataga
     8221 gtcactttct gaagatcgag ctcgtttgct tgaggaaaag aaaaagcttg aagaagaagt
     8281 cagtaagttg cgtagtagca gttttgttcc ttcaccatat gtagctacag ccccagaact
     8341 ttatggagct tgtgcacctg aactcccagg tgaatcagat agatccgctg tggaaacagc
     8401 agatgaagga agagtggatt cagcaatgga gacaagcatg atgtctgtac aagaaaatat
     8461 tcatatgttg tctgaagaaa aacagcggat aatgctgtta gaacgaacat tgcaattgaa
     8521 agaagaagaa aataaacggt taaatcaaag actgatgtct cagagcatgt cttcagtatc
     8581 ttcaaggcat tctgaaaaga tagctattag agattttcag gtgggagatt tggtactcat
     8641 catcctagac gaacgccatg acaattatgt gttatttact gttagtccta ctttatattt
     8701 tctacattca gagtctctac ctgccctgga tctcaaacca ggtgagggtg cttcaggtgc
     8761 atctagaaga ccctgggtac ttggaaaagt aatggaaaaa gaatactgtc aagccaaaaa
     8821 ggcacaaaac agatttaaag ttcctttggg gacaaagttt tacagagtga aagccgtatc
     8881 atggaataag aaagtatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     8941 gctttatata tcttgtggaa aggacgaaaa gaagcggagg gatggtaagt tacgcgttaa
     9001 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     9061 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     9121 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     9181 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     9241 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     9301 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     9361 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     9421 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     9481 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     9541 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     9601 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     9661 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     9721 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     9781 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9841 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9901 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     9961 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    10021 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10081 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
    10141 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    10201 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    10261 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
    10321 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    10381 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    10441 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
    10501 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    10561 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    10621 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    10681 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    10741 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    10801 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    10861 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    10921 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    10981 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    11041 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    11101 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    11161 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    11221 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    11281 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    11341 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    11401 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    11461 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    11521 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    11581 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    11641 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    11701 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    11761 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    11821 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    11881 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    11941 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    12001 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    12061 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    12121 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    12181 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    12241 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    12301 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    12361 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    12421 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    12481 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    12541 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    12601 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    12661 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    12721 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    12781 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    12841 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    12901 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    12961 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    13021 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    13081 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    13141 agctgcaagc
//
