LOCUS       sequence-396239-       13141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1316
FEATURES             Location/Qualifiers
     source          1..13141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6796..6807
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             7039..7050
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    8998..9586
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9051..9071)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9469..9480)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9658..9891
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9969..10090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10006..10025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10060..10079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10130..10265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10285..10304)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10286..10304)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10314..10331)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10314..10330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10323..10345)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10472..10927
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10559..10578)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10769..10790
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10953..11057
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11058..11126,11127..11918)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11276..11295)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12089..12677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12578..12597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12831..12848
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12965..12986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13001..13006,13007..13024,13025..13031)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13039..13055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13044..13066
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13063..13079
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13063..13079
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13098..13118
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13100..13118
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaag ttatatgtat ttctggttaa
     4141 cactggaact actctaacat ttgacactga acttacagtg caaactgtgg cagaccttaa
     4201 gcatgccatt caaagcaaat acaagattgc tattcaacac caggtgctgg tggtcaatgg
     4261 aggagaatgc atggctgcag atcgaagagt gtgtacctac agtgctggga cggatacaaa
     4321 tccaattttt ctttttaaca aagaaatgat cttatgtgat cgtccacctg ctattcctaa
     4381 aactaccttt tcgacagaaa atgacatgga aataaaagtt gaagaatctc ttatgatgcc
     4441 tgcagttttt catactgttg cttcaaggac acagcttgca ttggaaatgt atgaagttgc
     4501 caagaaactt tgttcttttt gtgaaggtct tgtacatgat gaacatcttc aacaccaagg
     4561 ctgggctgca atcatggcca acctggagga ctgttcaaat tcataccaaa agctactttt
     4621 caagtttgaa agtatttatt caaattatct gcagtccata gaagacatca agttaaaact
     4681 tactcattta ggaactgcag tttcagtaat ggccaagatt ccactgttgg agtgcctaac
     4741 cagacatagt tacagagaat gtttgggaag actggattct ttacctgaac atgaagactc
     4801 agaaaaagct gagatgaaaa gatccactga actggtgctc tctcctgata tgcctagaac
     4861 aactaacgaa tctttgttaa cctcatttcc caagtcagtg gaacatgtgt ccccagatac
     4921 cgcagatgct gaaagtggca aagaaattag ggaatcttgt caaagtactg ttcatcagca
     4981 agatgaaact acgattgaca ctaaagatgg tgatctgccc ttttttaatg tctctttgtt
     5041 agactggata aatgttcaag atagacctaa tgatgtggaa tctttggtca ggaagtgctt
     5101 tgattctatg agcaggcttg atccaaggat tattcgacca tttatagcag aatgccgtca
     5161 aactattgcc aaacttgata atcagaatat gaaagccatt aaaggacttg aagatcggct
     5221 ctacgccctg gaccagatga ttgctagctg tggccgactg gtgaatgaac agaaagagct
     5281 tgctcaggga tttttagcta atcagaagag agctgaaaac ttaaaggatg catctgtatt
     5341 acctgattta tgcctgagtc acgcaaatca gttgatgatt atgttgcaaa atcatagaaa
     5401 actgttagat attaagcaga agtgtaccac tgccaaacaa gaactagcaa ataacctaca
     5461 tgtcagactg aagtggtgtt gctttgtaat gcttcatgct gatcaagatg gagagaagtt
     5521 acaagctttg ctccgcctcg taatagagct gttagaaaga gtcaaaattg ttgaagctct
     5581 tagtacagtt cctcagatgt actgcttagc tgttgttgag gttgtaagaa gaaaaatgtt
     5641 cataaaacac tacagggagt gggctggtgc tttagtcaaa gatggaaaga gattatatga
     5701 agcagaaaaa tcaaaaaggg aatcctttgg gaaattattt aggaagtctt ttttaagaaa
     5761 tcgtctgttt aggggactgg actcctggcc cccttccttt tgtactcaaa agcctcgaaa
     5821 gtttgactgt gaacttccag atatttcatt aaaagattta cagtttctgc aatcattttg
     5881 tccttcggaa gttcagccat tcctcagggt tcccttactt tgtgactttg aacctctaca
     5941 ccagcatgta cttgctctac ataatttggt aaaagcagca caaagtttgg atgaaatgtc
     6001 acagaccatt acagatctac tgagtgaaca aaaggcatct gtgagtcaga catccccaca
     6061 gtctgcttct tcaccaagga tggaaagtac agcaggaatt acaactacta cctcaccgag
     6121 aactcctcca ccactgactg ttcaggatcc cttatgtcct gcagtttgtc ccttagaaga
     6181 attatctcca gatagtattg atgcacatac gtttgatttt gaaactattc cccatccaaa
     6241 catagaacag actattcacc aagtttcttt agacttggat tcattagcag aaagtcctga
     6301 atcagatttt atgtctgctg tgaatgagtt tgtaatagaa gaaaatttgt cgtctcctaa
     6361 tcctataagt gatccacaaa gcccagaaat gatggtggaa tcactttatt catcagttat
     6421 caatgcgata gacagtagac gaatgcagga tacaaatgta tgtggtaagg aggattttgg
     6481 agatcatact tctctgaatg tccagttgga aagatgtaga gttgttgccc aagactctca
     6541 cttcagtata caaaccatta aggaagacct ttgccacttt agaacatttg tacaaaaaga
     6601 acagtgtgac ttctcaaatt cattaaaatg tacagcagta gaaataagaa acattattga
     6661 aaaagtaaaa tgttctctgg aaataacact aaaagaaaaa catcaaaaag aactactgtc
     6721 tttaaaaaat gaatatgaag gtaaacttga cggactaata aaggaaactg aagagaatga
     6781 aaacaaaatt aaaaaattga agggagagtt agtatgcctt gaggaggttt tacaaaataa
     6841 agataatgaa tttgctttgg ttaaacatga aaaagaagct gtaatctgcc tgcagaatga
     6901 aaaggatcag aagttgttag agatggaaaa tataatgcac tctcaaaatt gtgaaattaa
     6961 agaactgaag cagtcacgag aaatagtgtt agaagactta aaaaagctcc atgttgaaaa
     7021 tgatgagaag ttacagttat tgagggcaga acttcagtcc ttggagcaaa gtcatctaaa
     7081 ggaattagag gacacacttc aggttaggca catacaagag tttgagaagg ttatgacaga
     7141 ccacagagtt tctttggagg aattaaaaaa ggaaaaccaa caaataatta atcaaataca
     7201 agaatctcat gctgaaatta tccaggaaaa agaaaaacag ttacaggaat taaaactcaa
     7261 ggtttctgat ttgtcagaca cgagatgcaa gttagaggtt gaacttgcgt tgaaggaagc
     7321 agaaactgat gaaataaaaa ttttgctgga agaaagcaga gcccagcaga aggagacctt
     7381 gaaatctctt cttgaacaag agacagaaaa tttgagaaca gaaattagta aactcaacca
     7441 aaagattcag gataataatg aaaattatca ggtgggctta gcagagctaa gaactttaat
     7501 gacaattgaa aaagatcagt gtatttccga gttaattagt agacatgaag aagaatctaa
     7561 tatacttaaa gctgaattaa acaaagtaac atctttgcat aaccaagcat ttgaaataga
     7621 aaaaaaccta aaagaacaaa taattgaact gcagagtaaa ttggattcag aattgagtgc
     7681 tcttgaaaga caaaaagatg aaaaaattac ccaacaagaa gagaaatacg aagctattat
     7741 ccagaacctt gagaaagaca gacaaaaatt ggtcagcagc caggagcaag acagagaaca
     7801 gttaattcag aagcttaatt gtgaaaaaga tgaagctatt cagactgccc taaaagaatt
     7861 taaattggag agagaagttg ttgagaaaga gttattagaa aaagttaaac atcttgagaa
     7921 tcaaatagca aaaagtcctg ccattgactc taccagagga gattcttcaa gcttagttgc
     7981 tgaacttcaa gaaaagcttc aggaagaaaa agctaagttt ctagaacaac ttgaagagca
     8041 agaaaaaaga aagaatgaag aaatgcaaaa tgttcgaaca tctttgattg cggaacaaca
     8101 gaccaatttt aacactgttt taacaagaga gaaaatgaga aaagaaaaca taataaatga
     8161 tcttagtgat aagttgaaaa gtacaatgca gcaacaagaa cgggataaag atttgataga
     8221 gtcactttct gaagatcgag ctcgtttgct tgaggaaaag aaaaagcttg aagaagaagt
     8281 cagtaagttg cgtagtagca gttttgttcc ttcaccatat gtagctacag ccccagaact
     8341 ttatggagct tgtgcacctg aactcccagg tgaatcagat agatccgctg tggaaacagc
     8401 agatgaagga agagtggatt cagcaatgga gacaagcatg atgtctgtac aagaaaatat
     8461 tcatatgttg tctgaagaaa aacagcggat aatgctgtta gaacgaacat tgcaattgaa
     8521 agaagaagaa aataaacggt taaatcaaag actgatgtct cagagcatgt cttcagtatc
     8581 ttcaaggcat tctgaaaaga tagctattag agattttcag gtgggagatt tggtactcat
     8641 catcctagac gaacgccatg acaattatgt gttatttact gttagtccta ctttatattt
     8701 tctacattca gagtctctac ctgccctgga tctcaaacca gcttcaggtg catctagaag
     8761 accctgggta cttggaaaag taatggaaaa agaatactgt caagccaaaa aggcacaaaa
     8821 cagatttaaa gttcctttgg ggacaaagtt ttacagagtg aaagccgtat catggaataa
     8881 gaaagtatag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     8941 atcttgtgga aaggacgaat acttcgagag gaaattcttg ggacgcgtta agtcgacaat
     9001 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     9061 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     9121 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     9181 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     9241 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     9301 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     9361 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     9421 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     9481 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     9541 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     9601 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     9661 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     9721 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9781 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9841 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     9901 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     9961 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    10021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    10081 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
    10141 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    10201 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
    10261 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
    10321 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
    10381 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    10441 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
    10501 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    10561 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    10621 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    10681 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    10741 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    10801 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    10861 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    10921 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10981 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    11041 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    11101 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    11161 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    11221 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    11281 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    11341 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    11401 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    11461 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    11521 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    11581 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    11641 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    11701 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    11761 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    11821 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    11881 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    11941 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    12001 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    12061 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    12121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    12181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    12241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    12301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    12361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    12421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    12481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    12541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    12601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    12661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    12721 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    12781 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    12841 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    12901 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    12961 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    13021 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    13081 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    13141 c
//
