LOCUS       sequence-396245-       13429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1369
FEATURES             Location/Qualifiers
     source          1..13429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    9286..9874
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9339..9359)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9757..9768)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9946..10179
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10257..10378
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10294..10313)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10348..10367
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10418..10553
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10573..10592)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10574..10592)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10602..10619)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10602..10618)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10611..10633)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10760..11215
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10847..10866)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11057..11078
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11241..11345
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11346..11414,11415..12206)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11564..11583)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12377..12965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12866..12885
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13119..13136
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13253..13274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13289..13294,13295..13312,13313..13319)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13327..13343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13332..13354
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13351..13367
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13351..13367
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13386..13406
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13388..13406
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgacg tcaagttcgc ccgctggctt
     4141 ggaaggttca gacctatctt ccatcaacac catgatgtcg gcggtcatga gtgtagggaa
     4201 ggtcacagag aatggcggga gcccccaggg gatcaagtcc ccctcgaagc ctccaggacc
     4261 aaatcggatt ggcagaagga accaggaaac gaaagaggag aagtcttcct ataactgccc
     4321 cctgtgtgag aagatttgca ctacccagca ccagctgacc atgcacattc gccagcacaa
     4381 cacagacact ggaggagccg accactcatg cagcatctgc ggaaagtcac tgagctcggc
     4441 cagctccctc gatcgccaca tgctggtgca ctctggcgag aggccttaca agtgcactgt
     4501 gtgtggccag tcatttacca ccaatgggaa catgcacaga catatgaaga tccatgagaa
     4561 ggaccctaac agtgccacag ccacagcccc tccatctcct ctgaaacgta ggcgattgtc
     4621 ctccaagagg aaactgagtc acgatgccga gtcagagaga gaagacccag caccagctaa
     4681 aaagatggta gaagacgggc agtcaggtga cttggagaag aaagctgatg aagtctttca
     4741 ctgcccagta tgtttcaagg agtttgtttg caagtatgga ctggagaccc acatggagac
     4801 ccattcagat aacccactaa gatgtgacat ttgttgtgtc acctttcgaa cacatcgagg
     4861 actgctgcgt cacaacgcgc ttgtccacaa acaacttccc agggatgcaa tgggcagacc
     4921 tttcatacag aacaaccctt caattcctgc tggcttccac gacttaggat tcacggactt
     4981 ctcctgtagg aagtttcctc gcatttctca ggcctggtgc gaaacaaacc tgcggaggtg
     5041 catcagcgag caacaccgtt ttgtctgcga cacctgtgac aaggcgttcc ccatgctctg
     5101 ctcactggct ctgcacaagc agacccatgt ggcggcagac cagggtcaag aaaagccgca
     5161 ggccacgccc ctgcctggtg acgccctgga ccagaagggc ttcctggcct tgcttggcct
     5221 gcagcacacc aaagacgtca ggcctgcccc cgccgaggag cccctgccgg atgacaacca
     5281 ggcaattcag ctccagacac tcaagtgtca gctacctcag gaccccggct gcaccaacct
     5341 gctgagcctg tcacctttcg aagctgcttc cctaggcggt tctctcacag ttctccccgc
     5401 gaccaaggac agcataaagc acctgtccct gcagcccttc cagaagggct tcatcatcca
     5461 gcctgacagc agcattgtgg tcaagcccat ctctggcgag tcggccatcg agctggcaga
     5521 catccagcaa attctgaaga tggcagcctc ggctccccct cagatcagtc ttccgccctt
     5581 ctccaaggcc cctgccgccc cactgcaggc gatcttcaag cacatgcccc ctctgaagcc
     5641 aaagcccctg gtcacaccac ggacggtggt ggccacctcc acgcccccgc ctctcatcaa
     5701 cgcccagcag gcttccccgg gctgtatcag ccccagcctg ccgccaccgc ccctgaagct
     5761 cctcaaaggc tcagtggagg cggcctccaa cgcccacctg ctgcagtcca agtccgggac
     5821 ccagccccac gcggccacgc ggctctccct gcagcagccg cgggcggagc tgccgggcca
     5881 gcctgagatg aagacgcagc tggagcagga cagcatcatc gaggccctgc tgccgctgag
     5941 catggaggcc aagatcaagc aggagatcac agagggggaa ctcaaggcct tcatgacagc
     6001 gcccggcggc aagaagacgc ccgccatgcg caaggtgctc tacccctgcc gcttctgcaa
     6061 ccaggtgttt gccttctcgg gggtcttgcg tgcccacgtg cgctcccacc tgggcatctc
     6121 gccataccag tgcaacatct gcgactacat cgccgccgac aaggccgcgc tcatccgcca
     6181 cctgcgcacg cacagtgggg agcggcccta catttgcaag atctgccact accccttcac
     6241 tgtcaaagcc aactgcgagc ggcacctgcg caagaagcac ctcaaggcca cccgcaagga
     6301 tatcgagaag aacatcgagt atgtgagtag cagcgcggcc gagctggtgg acgccttctg
     6361 cgccccggac accgtgtgcc ggctgtgcgg cgaggacctc aagcactatc gtgccctgcg
     6421 catccacatg cgcacgcact gcggccgcgg cctgggcggg ggccacaagg gccgcaagcc
     6481 cttcgagtgc aaggagtgca gcgccgcgtt cgcggccaag cgcaactgca tccaccacat
     6541 cctcaagcag cacctgcacg tgcccgagca ggacatcgag agctacgtgc tggccgccga
     6601 cggcctgggc cccgcagagg cgccggccgc tgaggcgtcg gggcgcgggg aggacagtgg
     6661 ctgcgctgcc cttggtgact gcaagcccct cactgccttc ctggaacccc agaacggctt
     6721 tcttcacagg ggccccaccc agcctccacc tccccatgtc tcgatcaagt tggagcccgc
     6781 cagtagcttt gcggtggact tcaatgagcc cctggacttc tcgcagaagg gcctggccct
     6841 ggtccaagtg aagcaggaaa acatctcctt tctgagccct tcttccctgg tcccctatga
     6901 ctgctccatg gagcccatcg acctgtccat ccccaagaac ttcaggaaag gggacaagga
     6961 tttggccact cccagcgaag ccaagaagcc tgaggaggag gcggggagca gcgagcagcc
     7021 ctctccctgc ccagcacccg gcccttctct tcctgtaact ttggggccca gcggaatcct
     7081 ggaaagcccc atggcccctg ctccggcggc caccccggaa cccccagcac agcccctgca
     7141 gggccctgtt cagctggcgg tcccaatcta ctcctcagcc ctggtcagca gccctccact
     7201 cgtgggcagc tcagccctcc tgagtggcac agccttgctg cgtccactgc ggcccaagcc
     7261 cccgctgctt ttgccaaagc cccccgtgac agaagagctg cccccgctgg cctccattgc
     7321 ccagatcatc tcatctgtat cctcggcccc caccctgctg aaaaccaagg tggcggaccc
     7381 agggcccgca agcactggca gtaacaccac ggcttcagac agcttaggag gttctgtccc
     7441 caaagccgcc accaccgcca cccccgctgc caccaccagc ccaaaagagt ctagtgagcc
     7501 tcccgctcca gccagcagcc cagaggctgc ctctcccacc gagcagggcc cagcgggcac
     7561 gtcgaagaag aggggccgga aaagggggat gaggagccga ccccgcgcca acagcggcgg
     7621 ggtggacctg gactccagcg gggagtttgc cagcatcgag aagatgctgg ccaccacaga
     7681 caccaacaag ttcagtccgt ttctgcagac agcggaggac aacactcagg atgaggtggc
     7741 cggagcccct gccgaccacc atgggcccag tgatgaagag cagggcagtc ccccagaaga
     7801 caagctgctg agggccaagc ggaactcgta caccaactgc ctgcagaaga tcacctgtcc
     7861 ccactgtccc cgggttttcc cttgggccag ctccctacag aggcacatgc tcacacacac
     7921 tgacagtcag tcggatgcgg agactgcagc cgccgcgggc gaagtgctag acctcacctc
     7981 acgggacaga gagcagccgt cggagggcgc cactgagctc cgccaggtcg caggggatgc
     8041 gcctgtggag caggccacgg cggaaacggc ctcgccggtg caccgggaag agcacgggcg
     8101 tggggagagc catgagccgg aggaggagca tggcactgag gagagcactg gggacgccga
     8161 cggcgcggaa gaggacgcgt cgagcaacca gagcctggac ctggacttcg ccaccaagct
     8221 catggacttc aagctggcgg agggcgacgg cgaggcaggc gccgggggcg cggcctcgca
     8281 ggagcagaag ctcgcctgcg acacctgtgg gaagagcttc aagttcctgg gcaccctgag
     8341 ccgccaccgg aaggcgcacg gccgccagga gcccaaggac gagaagggag atggcgccag
     8401 cactgcagag gaggggcccc agcccgcccc tgaacaggag gagaagcccc ccgagacccc
     8461 ggcagaggtg gtggagtcgg ccccgggtgc cggggaggcc ccggcggaaa agctcgcgga
     8521 ggagacggag ggcccctccg acggggagag cgcggccgag aaaaggtcct cagagaagag
     8581 cgacgatgac aagaaaccaa agacagactc ccccaaaagc gtggccagca aggcagacaa
     8641 gaggaagaag gtctgcagcg tgtgcaacaa gcggttctgg tcgctgcagg acctgacccg
     8701 gcacatgcgc tcccacacag gggaaaggcc atacaaatgt cagacctgcg agcgaacctt
     8761 caccttgaag cacagcctgg ttcgccacca gcggatccac cagaaagcca ggcatgccaa
     8821 acaccacggg aaggacagcg acaaggaaga gcggggtgag gaggacagcg agaatgagtc
     8881 cacccacagc ggcaacaacg ccgtctcaga gaacgaggct gagctggctc ccaatgccag
     8941 caaccacatg gctgtcaccc ggagccggaa ggagggcttg gccagtgcca ccaaggactg
     9001 cagccacagg gaggagaagg tcacggcagg gtggccgtct gagcctggcc agggtgacct
     9061 taacccagag agcccggcgg ccctggggca ggacctgctg gagccgcgca gcaagaggcc
     9121 tgcccaccca atcctggcca cagctgatgg cgcctcccag ctcgtgggga tggagtagta
     9181 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     9241 ggacgattca ttcagcacat aatcagcata acgcgttaag tcgacaatca acctctggat
     9301 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     9361 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     9421 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     9481 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     9541 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     9601 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     9661 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     9721 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     9781 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     9841 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     9901 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     9961 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
    10021 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    10081 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    10141 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
    10201 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
    10261 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    10321 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    10381 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    10441 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    10501 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
    10561 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
    10621 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    10681 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    10741 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    10801 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    10861 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    10921 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    10981 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    11041 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    11101 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    11161 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
    11221 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    11281 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    11341 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    11401 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    11461 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    11521 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    11581 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    11641 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    11701 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    11761 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    11821 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    11881 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    11941 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    12001 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    12061 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    12121 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    12181 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    12241 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    12301 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    12361 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    12421 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    12481 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    12541 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    12601 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    12661 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    12721 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    12781 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    12841 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    12901 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    12961 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    13021 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    13081 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    13141 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    13201 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    13261 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    13321 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    13381 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
