LOCUS       sequence-396255-       12139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12139)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1440
FEATURES             Location/Qualifiers
     source          1..12139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7996..8584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8049..8069)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8467..8478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8656..8889
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8967..9088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9004..9023)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9058..9077
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9128..9263
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9283..9302)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9284..9302)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9312..9329)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9312..9328)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9321..9343)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9470..9925
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9557..9576)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9767..9788
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9951..10055
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10056..10124,10125..10916)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10274..10293)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11087..11675
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11576..11595
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11829..11846
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11963..11984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11999..12004,12005..12022,12023..12029)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12037..12053
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12042..12064
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12061..12077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12061..12077
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12096..12116
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12098..12116
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaag gcggcagatg agcctgccta
     4141 cctgacagtg ggaaccgatg tcagtgccaa gtaccgaggt gccttctgtg aggcaaagat
     4201 taagactgtg aaaaggctgg tgaaagttaa ggtactcctg aaacaggata ataccacaca
     4261 attggtacaa gatgaccaag taaagggtcc tttaagagtt ggagctattg ttgaaacaag
     4321 gacatctgat ggatcttttc aggaagctat tatcagcaag ttgacagatg ctagttggta
     4381 taccgtggtg tttgatgatg gtgatgagcg aacattgaga cgtacctcac tttgtctgaa
     4441 aggagagaga cattttgcag agagtgagac acttgaccag cttccattaa caaatccaga
     4501 gcattttgga actccagtaa ttgcaaagaa gacgaacaga ggaaggagat cttctcttcc
     4561 tgttactgaa gatgaaaagg aagaagaaag cagtgaagag gaagatgaag acaagcgccg
     4621 tctcaatgat gaattactag gaaaagttgt aagtgtggtg tctgcaacgg agaggactga
     4681 atggtatcct gctttggtaa tatctcccag ctgtaatgat gacatcacag tgaaaaagga
     4741 tcagtgttta gttcgatcat ttattgattc taaattttac tctatagcaa gaaaggacat
     4801 taaggaagta gacattctca atctaccgga atctgagctc tccactaaac cagggcttca
     4861 gaaagcaagc atcttcttaa aaactagagt tgttcctgat aattggaaaa tggatataag
     4921 tgaaatcctt gagtcatcca gtagtgatga tgaagatggc ccagctgaag aaaatgatga
     4981 agagaaggaa aaggaggcca aaaagacaga agaagaggtg cctgaggaag aacttgatcc
     5041 tgaagagagg gacaacttcc tccagcagct ttataagttt atggaagaca gaggtactcc
     5101 aatcaacaaa ccacctgttt tgggctataa agatctcaat ctcttcaaac tcttcagact
     5161 ggtttatcat cagggtggat gtgacaatat tgatagtggt gctgtatgga agcaaattta
     5221 tatggacctt ggcattccta ttttgaattc agctgcttcc tacaatgtaa aaactgctta
     5281 tagaaagtat ctctatggtt ttgaggagta ctgccgttcg gcaaatattc agttcagaac
     5341 tgttcatcac catgaaccaa aagtaaaaga ggaaaaaaaa gacttagaag aatcaatgga
     5401 agaggctctc aaattagatc aagaaatgcc tttaacagaa gtgaagagtg aacctgagga
     5461 aaatatcgat tcaaacagtg aaagtgaaag agaagagata gaattaaaat ctccgagggg
     5521 acgaaggaga attgctcgag atgtaaattc tattaaaaag gaaattgaag aagagaaaac
     5581 agaagacaaa ttaaaagata atgatacaga aaataaggat gtagatgatg actatgaaac
     5641 tgcagagaaa aaagaaaatg agctactact ggggagaaaa aatacaccaa agcaaaaaga
     5701 gaagaaaatt aaaaaacagg aggattctga caaagactca gatgaagagg aagagaaaag
     5761 ccaagagagg gaagaaactg aaagcaaatg tgactctgaa ggagaggaag atgaggaaga
     5821 catggaaccc tgcctaacag gaaccaaagt gaaagtaaaa tatggacgag ggaagactca
     5881 gaaaatttat gaagccagta ttaaaagcac tgaaattgat gacggagaag ttttatattt
     5941 ggtacattac tatggatgga atgtcaggta tgatgagtgg gtgaaggctg acaggataat
     6001 ctggcctttg gacaaaggtg gaccaaagaa aaaacagaag aaaaaagcta aaaataaaga
     6061 agatagtgaa aaggacgaaa agagagatga ggagaggcag aagtcaaaac ggggacgacc
     6121 tcctttaaaa tcaaccctct catcaaacat gccgtatggc ttatctaaga cagcaaacag
     6181 tgaaggaaaa tcagactctt gttcatctga tagtgaaaca gaagatgctt tagaaaagaa
     6241 tttaataaat gaagaacttt ctcttaaaga tgaactagaa aaaaatgaaa atttgaatga
     6301 tgataagcta gatgaagaaa atccaaagat ttctgcacat atattaaaag aaaatgatag
     6361 gactcaaatg cagcctttag aaaccctgaa gttagaagtt ggagagaatg aacaaatagt
     6421 acagattttt gggaacaaaa tggaaaaaac agaagaagtt aagaaagaag ccgaaaaatc
     6481 tccaaaagga aagggaagac gaagcaagac aaaagatctt tctttagaaa ttataaagat
     6541 ttcatcattt ggccagaatg aagcaggaag tgaacctcat atagaagctc atagtcttga
     6601 attgtcttca ttagacaata aaaacttttc ttctgctaca gaagatgaaa ttgaccaatg
     6661 tgtgaaagaa aagaagttga aacggaaaat actaggacaa tcatcgccag agaaaaaaat
     6721 aagaattgag aatggaatgg aaatgacaaa tactgtatct caagaaagga ccagtgattg
     6781 tattggatct gagggaatga aaaacttaaa ttttgaacag cactttgaaa gagaaaatga
     6841 aggaatgcca tcattgatag cagagtcaaa ccaatgcatc caacaactga ctagtgaaag
     6901 atttgatagt ccagctgaag aaactgtaaa tattccacta aaagaagatg aggatgcaat
     6961 gcctctgatc gggcctgaaa ccttggtttg ccatgaagta gatttggatg atttggatga
     7021 aaaggataag accagcattg aggatgtagc agttgaaagc tctgagtcta actctcttgt
     7081 ttctattcca cctgccctac ctcctgtagt ccaacataac ttttcagtag cttcaccact
     7141 tactcttagt caagatgagt ctcgaagcgt aaaaagtgag agtgatataa cgattgaagt
     7201 tgatagtatt gctgaagaat ctcaagaagg tctctgtgag agggaatcgg caaatggatt
     7261 tgaaactaat gttgcctctg gtacctgtag tataattgta caagagagag agagcagaga
     7321 gaagggtcag aagaggccaa gtgatggaaa tagtggatta atggcaaaaa agcaaaagcg
     7381 taccccaaag cgaacaagtg ctgcagccaa aaatgaaaag aatggaacag gacaaagcag
     7441 tgatagtgaa gatcttcctg tcctagacaa ttcaagtaaa tgtaccccag taaagcatct
     7501 taatgtatct aagccacaga aacttgcacg atctcctgca agaatatccc cgcacatcaa
     7561 agatggagag aaagataaac acagagaaaa acatccgaat tcatccccta ggacatataa
     7621 atggagcttt cagctcaatg aattagataa tatgaacagt acagagagaa tctcatttct
     7681 ccaagaaaaa ctacaggaaa tcagaaaata ttatatgtct ttgaagtctg aagttgcaac
     7741 catagacagg aggagaaaaa gattaaaaaa gaaagacagg gaagtgtctc atgcgggagc
     7801 ctccatgtca tctgcttcat cagacactgg aatgagtccc tcatcatcat ctcccccaca
     7861 aaatgtactt gctgtagaat gcaggtagta atgaactagt ccgtaacttg aaagtatttc
     7921 gatttcttgg ctttatatat cttgtggaaa ggacgaaaac taacttcggg cgcgtctcga
     7981 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     8041 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     8101 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     8161 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     8221 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8281 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8341 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     8401 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     8461 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8521 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8581 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8641 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     8701 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8761 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8821 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8881 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     8941 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     9001 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9061 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     9121 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9181 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9241 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     9301 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     9361 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     9421 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     9481 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9541 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9601 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9661 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     9721 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     9781 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     9841 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     9901 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9961 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    10021 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    10081 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    10141 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    10201 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    10261 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    10321 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    10381 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    10441 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    10501 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    10561 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    10621 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10681 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    10741 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    10801 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    10861 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10921 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10981 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    11041 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    11101 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    11161 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    11221 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    11281 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    11341 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    11401 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    11461 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    11521 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    11581 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    11641 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    11701 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    11761 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    11821 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    11881 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    11941 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    12001 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    12061 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    12121 aaaagctgga gctgcaagc
//
