LOCUS       sequence-39626-e         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1149
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcggaagt tcctatacct tttgaagaat aggaacttcg
       61 gaataggaac ttcggcgcgc cggaacctct ggatctgcca gtcagccacc ctgtcctgct
      121 cagactttac cagagacgag tatttccctt tccttccctc taagagcatt ttatttgcag
      181 agttagccaa tagtatcttg tttcctgtaa gggagactat ctttagatgc tgccacaggc
      241 aagctcagat actccagggt ggaggccagc tgccaacagg agggcctgac tttataaacc
      301 tggaagatct aaacctcagg gaagactgcg gagctggagg gagagaggtg ccacttaccc
      361 caacctggag gtggggggca gggagcaggc cctgcagtca gcaaagcaca gcacagtcag
      421 ctctcattaa tggggagcct tcaggaagct gggagagctc atggggccct cagcttccct
      481 gcccatgaga gggagcccag agtactcact ggaccacact ggggcacaca cagctcttct
      541 cctcaaagtg cagtcccagg cactgggagc ttattaaaga cagcacctca agccctgccc
      601 tatatctgct gaattagaat ctgc
//
