LOCUS       sequence-396266-       12265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12265)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1524
FEATURES             Location/Qualifiers
     source          1..12265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8122..8710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8175..8195)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8593..8604)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8782..9015
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9093..9214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9130..9149)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9184..9203
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9254..9389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9409..9428)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9410..9428)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9438..9455)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9438..9454)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9447..9469)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9596..10051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9683..9702)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9893..9914
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10077..10181
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10182..10250,10251..11042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10400..10419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11213..11801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11702..11721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11955..11972
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12089..12110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12125..12130,12131..12148,12149..12155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12163..12179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12168..12190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12187..12203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12187..12203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12222..12242
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12224..12242
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggat tccttagacc atatgctgac
     4141 agatcctctg gaacttggtc cgtgtggaga tggccatggc acgcgcatca tggaggattg
     4201 cctcctggga ggcaccagag ttagtctgcc cgaggacctt ctggaggatc ctgagatctt
     4261 ctttgatgtt gtcagcctct caacatggca ggaagtgtta agtgattctc aacgtgaaca
     4321 cctccagcag tttctgcccc agtttcctga agacagtgct gagcagcaga atgaactcat
     4381 cttagccttg ttcagtgggg agaacttccg ctttggaaac cctctgcaca ttgcccagaa
     4441 gcttttccga gacggacact ttaaccccga ggtggtcaag taccggcagt tatgcttcaa
     4501 gtcacagtac aagcgctacc tcaactccca gcagcagtat ttccatcggc tgctgaagca
     4561 aattcttgct tcccggagtg atctgctgga gatggcccgg cggagtggcc ccgcccttcc
     4621 cttccggcag aaacgccctt caccatcccg cacacctgag gagcgggagt ggcggaccca
     4681 gcagcgctac ttgaaggtct taagggaagt gaaagaggag tgtggtgaca cagccctgtc
     4741 atctgatgaa gaggatctca gctcatggct tccgagctct ccagcacgtt ctcctagtcc
     4801 tgcggtgccc ctgcgggtgg tgcccacact ttcaaccacg gatatgaaaa ctgcagataa
     4861 agtagaactg ggggacagtg acctgaagat aatgttaaag aagcaccacg agaagcggaa
     4921 acatcagcca gatcacccgg accttttgac aggggacctg actctcaatg acatcatgac
     4981 tcgagtaaat gctggcagga agggctctct ggcagcctta tatgacttgg ctgtccttaa
     5041 aaaaaaggtt aaggaaaaag aggaaaagaa gaagaagaaa ataaaaacga tcaaatcaga
     5101 ggcagaggac ctggccgagc cgctaagcag tactgaaggg gtcgcacctc tctcacaggc
     5161 cccctctccg ctggcaattc ctgctatcaa ggaagagccc cttgaagacc tcaagccttg
     5221 ccttggaatc aatgaaatat cttccagctt cttctctctt ctattagaga tcttgctgct
     5281 ggagagtcag gctagccttc ctatgctaga ggagcgagtt ttggattggc agtcatcgcc
     5341 agccagctcc ctcaacagct ggttctctgc ggcccccaac tgggctgagt tggtactacc
     5401 agccctgcag tatcttgctg gagaaagtcg agctgttcct tccagtttct ctccatttgt
     5461 tgaattcaaa gagaaaaccc agcagtggaa gttgcttggc caatcccaag ataatgaaaa
     5521 ggaattagct gccctcttcc agctatggct agagaccaaa gatcaggcct tctgtaagca
     5581 agaaaatgaa gacagctcag atgccacaac acctgtccct cgggtaagaa ctgactatgt
     5641 ggtgcgtccc agcacggggg aggagaaacg ggtttttcag gagcaggagc gttacaggta
     5701 tagccaaccc cataaggcgt tcacctttcg catgcacggc tttgagtctg tggtggggcc
     5761 agtgaagggc gtgtttgaca aggagacctc gctcaacaag gctcgggagc actccctgct
     5821 gcgctccgac cggcctgcct acgtcaccat tctgtctctt gttcgggacg ctgcggctcg
     5881 actgcctaat ggagaaggca cacgggcaga gatctgtgaa ctgcttaagg actcccagtt
     5941 tcttgcacca gatgtcacca gcactcaggt aaatacagta gtgagtggtg cactggatcg
     6001 gctacattac gaaaaagatc cctgtgtgaa atacgacatt ggacgaaagc tgtggatcta
     6061 cctgcatcgt gaccggagtg aagaagagtt tgagcggatt caccaagcac aagcagctgc
     6121 agctaaagcc agaaaagctc ttcagcaaaa acccaagccc ccatccaagg tgaagtccag
     6181 tagcaaggag agctccataa aggtccttag cagtggccct tctgagcaga gccagatgag
     6241 cctcagtgac tccagtatgc cacccacccc agtcacacct gtaaccccca ccacaccagc
     6301 attgcccgcc attcccatct cccctccacc tgtatcggca gtgaacaaaa gcggcccttc
     6361 cacagtctca gaaccagcta agtctagctc gggtgttctt ctggtgtctt caccaacaat
     6421 gccacatctg ggaacaatgc tttccccagc ttccagccag actgcaccca gttctcaggc
     6481 tgccgcccgg gtcgtgagcc actctggctc tgctggactg tctcaggtgc gagtggtggc
     6541 ccagcctagc cttcctgctg ttccccagca gtcgggaggg ccggcacaga cattgccaca
     6601 gatgccagca ggaccgcaga tccgggttcc agccactgcc acacagacca aagtagtgcc
     6661 ccagacagta atggccactg tgcccgtcaa agcgcagact acggcagcca ctgtgcagcg
     6721 gcctggaccc gggcagacag ggctcacggt gacaagtctc cctgccacag ccagccctgt
     6781 gagtaagcca gccacgagtt ctcctgggac ctctgctccc agtgcctcca cggctgccgt
     6841 cattcaaaat gtcacaggac agaacatcat caagcaggtg gcaatcactg ggcagcttgg
     6901 tgtgaagccc caaacaggca acagcattcc actcacagcc actaacttcc gcatccaggg
     6961 taaggatgta ttgcgtctgc cgccctcttc catcaccaca gatgccaagg gccagacggt
     7021 tctgcgaatc actccggaca tgatggccac attggccaag tcccaggtta ccacagtcaa
     7081 attgacccag gacctcttcg ggacaggagg caacactaca ggcaaaggca tctctgccac
     7141 cttacacgtc acttccaatc cagtacatgc agctgatagc cctgccaagg ccagttcagc
     7201 cagtgcccct tcatccactc caacaggtac cactgtggtc aaagtgactc ctgacctcaa
     7261 gccaacagaa gcctcaagtt cggcttttcg cttgatgcca gctcttggcg tgagtgtggc
     7321 tgaccagaag ggaaaaagca cagtggcctc ttcagaagca aaaccagctg ccacgatccg
     7381 catcgtgcag ggactgggag tgatgcctcc caaagcaggc cagaccatca ccgttgcaac
     7441 ccacgccaag caaggggcct cggtggccag tgggtctgga actgtccata cttcagcggt
     7501 gtccttaccc agtatgaatg ctgctgtgtc caagactgta gctgtggctt ctggggctgc
     7561 aagcaccccc atcagcatca gcacaggagc ccccaccgtg cggcaggtcc ctgtcagcac
     7621 cacggttgtg tccacgtccc aggctgggaa gttgcctaca cggatcacag ttcccctctc
     7681 tgtgatcagc cagccaatga agggcaagag cgtggtcaca gcccccatca tcaaaggcaa
     7741 ccttggagcc aacctcagtg ggttgggccg caacatcatc ctcacaacta tgccagcagg
     7801 cactaagctc attgctggca ataagcctgt tagtttcctc actgctcagc agttgcagca
     7861 gcttcagcag caaggtcagg ccacacaggt gcgcatccag actgtccctg catcccatct
     7921 ccaacaggga acagcttctg gctcctccaa agcagtctcc actgttgttg tgactacagc
     7981 tccgtctcct aaacaggcac ctgagcaaca atagtaatga actagtccgt aacttgaaag
     8041 tatttcgatt tcttggcttt atatatcttg tggaaaggac gacacagagt gtagtcaatt
     8101 aacgccacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     8161 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8221 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8281 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8341 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8401 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8461 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     8521 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8581 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8641 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8701 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     8761 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     8821 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8881 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8941 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9001 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     9061 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     9121 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9181 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     9241 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9301 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9361 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     9421 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     9481 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     9541 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     9601 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     9661 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     9721 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     9781 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     9841 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     9901 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     9961 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    10021 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
    10081 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10141 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10201 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10261 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10321 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10381 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    10441 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10501 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    10561 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    10621 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    10681 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    10741 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    10801 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    10861 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    10921 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    10981 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    11041 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    11101 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    11161 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    11221 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    11281 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    11341 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    11401 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    11461 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    11521 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    11581 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    11641 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    11701 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    11761 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    11821 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    11881 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    11941 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    12001 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    12061 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    12121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    12181 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    12241 gggaacaaaa gctggagctg caagc
//
