LOCUS       sequence-396268-       10354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10354)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1689
FEATURES             Location/Qualifiers
     source          1..10354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5585..5594
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6211..6799
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6264..6284)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6682..6693)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6871..7104
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7182..7303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7219..7238)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7273..7292
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7343..7478
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7498..7517)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7499..7517)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7527..7544)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7527..7543)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7536..7558)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7685..8140
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7772..7791)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7982..8003
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8166..8270
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8271..8339,8340..9131)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8489..8508)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9302..9890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9791..9810
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10044..10061
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10178..10199
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10214..10219,10220..10237,10238..10244)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10252..10268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10257..10279
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10276..10292
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10276..10292
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10311..10331
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10313..10331
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggtt tctaaactga gccagctgca
     4141 gacggagctc ctggcggccc tgctcgagtc agggctgagc aaagaggcac tgatccaggc
     4201 actgggtgag ccggggccct acctcctggc tggagaaggc cccctggaca agggggagtc
     4261 ctgcggcggc ggtcgagggg agctggctga gctgcccaat gggctggggg agactcgggg
     4321 ctccgaggac gagacggacg acgatgggga agacttcacg ccacccatcc tcaaagagct
     4381 ggagaacctc agccctgagg aggcggccca ccagaaagcc gtggtggaga cccttctgca
     4441 ggaggacccg tggcgtgtgg cgaagatggt caagtcctac ctgcagcagc acaacatccc
     4501 acagcgggag gtggtcgata ccactggcct caaccagtcc cacctgtccc aacacctcaa
     4561 caagggcact cccatgaaga cgcagaagcg ggccgccctg tacacctggt acgtccgcaa
     4621 gcagcgagag gtggcgcagc agttcaccca tgcagggcag ggagggctga ttgaagagcc
     4681 cacaggtgat gagctaccaa ccaagaaggg gcggaggaac cgtttcaagt ggggcccagc
     4741 atcccagcag atcctgttcc aggcctatga gaggcagaag aaccctagca aggaggagcg
     4801 agagacgcta gtggaggagt gcaatagggc ggaatgcatc cagagagggg tgtccccatc
     4861 acaggcacag gggctgggct ccaacctcgt cacggaggtg cgtgtctaca actggtttgc
     4921 caaccggcgc aaagaagaag ccttccggca caagctggcc atggacacgt acagcgggcc
     4981 ccccccaggg ccaggcccgg gacctgcgct gcccgctcac agctcccctg gcctgcctcc
     5041 acctgccctc tcccccagta aggtccacgg tgtgcgctat ggacagcctg cgaccagtga
     5101 gactgcagaa gtaccctcaa gcagcggcgg tcccttagtg acagtgtcta cacccctcca
     5161 ccaagtgtcc cccacgggcc tggagcccag ccacagcctg ctgagtacag aagccaagct
     5221 ggtctcagca gctgggggcc ccctcccccc tgtcagcacc ctgacagcac tgcacagctt
     5281 ggagcagaca tccccaggcc tcaaccagca gccccagaac ctcatcatgg cctcacttcc
     5341 tggggtcatg accatcgggc ctggtgagcc tgcctccctg ggtcctacgt tcaccaacac
     5401 aggtgcctcc accctggtca tcggcctggc ctccacgcag gcacagagtg tgccggtcat
     5461 caacagcatg ggcagcagcc tgaccaccct gcagcccgtc cagttctccc agccgctgca
     5521 cccctcctac cagcagccgc tcatgccacc tgtgcagagc catgtgaccc agagcccctt
     5581 catggccacc atggctcagc tgcagagccc ccacgccctc tacagccaca agcccgaggt
     5641 ggcccagtac acccacacgg gcctgctccc gcagactatg ctcatcaccg acaccaccaa
     5701 cctgagcgcc ctggccagcc tcacgcccac caagcaggta aggtccaggc ctgctggccc
     5761 tcccttggcc tgtgacagag cccctcaccc ccacatcccc cgggctcagg aggctgctct
     5821 gctcccccag gtcttcacct cagacactga ggcctccagt gagtccgggc ttcacacgcc
     5881 ggcatctcag gccaccaccc tccacgtccc cagccaggac cctgccagca tccagcacct
     5941 gcagccggcc caccggctca gcgccagccc cacagtgtcc tccagcagcc tggtgctgta
     6001 ccagagctca gactccagca atggccagag ccacctgctg ccatccaacc acagcgtcat
     6061 cgagaccttc atctccaccc agatggcctc ttcctcccag tagtaatgaa ctagtccgta
     6121 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg atgaattaga
     6181 acctgtatgg tacgcacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     6241 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6301 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6361 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6421 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6481 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6541 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6601 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6661 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6721 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6781 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     6841 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     6901 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6961 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7021 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7081 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     7141 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7201 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7261 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7321 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7381 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7441 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7501 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7561 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7621 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7681 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7741 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7801 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7861 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7921 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7981 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8041 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8101 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     8161 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8221 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8281 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8341 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8401 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8461 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8521 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8581 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8641 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8701 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8761 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8821 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8881 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8941 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9001 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9061 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9121 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9181 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9241 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9301 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9361 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9421 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9481 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9541 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9601 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9661 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9721 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9781 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9841 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9901 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9961 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    10021 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    10081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    10141 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    10201 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    10261 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    10321 ctcactaaag ggaacaaaag ctggagctgc aagc
//
