LOCUS       sequence-396287-       12304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12304)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1444
FEATURES             Location/Qualifiers
     source          1..12304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8161..8749
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8214..8234)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8632..8643)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8821..9054
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9132..9253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9169..9188)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9223..9242
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9293..9428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9448..9467)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9449..9467)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9477..9494)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9477..9493)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9486..9508)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9635..10090
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9722..9741)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9932..9953
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10116..10220
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10221..10289,10290..11081)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10439..10458)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11252..11840
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11741..11760
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11994..12011
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12128..12149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12164..12169,12170..12187,12188..12194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12202..12218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12207..12229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12226..12242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12226..12242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12261..12281
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12263..12281
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaag gcccttgatg agcctcccta
     4141 tttgacagtg ggcactgatg tgagtgctaa atacagagga gccttttgtg aagccaagat
     4201 caagacagca aaaagacttg tcaaagtcaa ggtgacattt agacatgatt cttcaacagt
     4261 ggaagttcag gatgaccaca taaagggccc actaaaggta ggagctattg tggaagtgaa
     4321 gaatcttgat ggtgcatatc aggaagctgt tatcaataaa ctaacagatg cgagttggta
     4381 cactgtagtt tttgatgacg gagatgagaa gacactgaga cgatcttcac tgtgcctgaa
     4441 aggagagagg cattttgctg aaagtgaaac attagaccag ctcccactca ccaaccctga
     4501 gcattttggc actccagtca taggaaagaa aacaaataga ggaagaagat ctaatcatat
     4561 accagaggaa gagtcttcat catcctccag tgatgaagat gaggatgata ggaaacagat
     4621 tgatgagcta ctaggcaaag ttgtatgtgt agattacatt agtttggata aaaagaaagc
     4681 actgtggttt cctgcattgg tggtttgtcc tgattgtagt gatgagattg ctgtaaaaaa
     4741 ggacaatatt cttgttcgat ctttcaaaga tggaaaattt acttcagttc caagaaaaga
     4801 tgtccatgaa attactagtg acactgcacc aaagcctgat gctgttttaa agcaagcctt
     4861 tgaacaggca cttgaatttc acaaaagtag aactattcct gctaactgga agactgaatt
     4921 gaaagaagat agctctagca gtgaagcaga ggaagaagag gaggaggaag atgatgaaaa
     4981 agaaaaggag gataatagca gtgaagaaga agaagaaata gaaccatttc cagaagaaag
     5041 ggagaacttt cttcagcaat tgtacaaatt tatggaagat agaggtacac ctattaacaa
     5101 acgacctgta cttggatatc gaaatttgaa tctctttaag ttattcagac ttgtacacaa
     5161 acttggagga tttgataata ttgaaagtgg agctgtttgg aaacaagtct accaagatct
     5221 tggaatccct gtcttaaatt cagctgcagg atacaatgtt aaatgtgctt ataaaaaata
     5281 cttatatggt tttgaggagt actgtagatc agccaacatt gaatttcaga tggcattgcc
     5341 agagaaagtt gttaacaagc aatgtaagga gtgtgaaaat gtaaaagaaa taaaagttaa
     5401 ggaggaaaat gaaacagaga tcaaagaaat aaagatggag gaggagagga atataatacc
     5461 aagagaagaa aagcctattg aggatgaaat tgaaagaaaa gaaaatatta agccctctct
     5521 gggaagtaaa aagaatttat tagaatctat acctacacat tctgatcagg aaaaagaagt
     5581 taacattaaa aaaccagaag acaatgaaaa tctggatgac aaagatgatg acacaactag
     5641 ggtagatgaa tccctcaaca taaaggtaga agctgaggaa gaaaaagcaa aatctggaga
     5701 tgaaacgaat aaagaagaag atgaagatga tgaagaagca gaagaggagg aggaggagga
     5761 agaagaagaa gaggatgaag atgatgatga caacaatgag gaagaggagt ttgagtgcta
     5821 tccaccaggc atgaaagtcc aagtgcggta tggacgaggg aaaaatcaaa aaatgtatga
     5881 agctagtatt aaagattctg atgtcgaagg tggagaggtc ctttacttgg tgcattactg
     5941 cggatggaat gtgagatacg atgaatggat taaagcagat aaaatagtaa gacctgctga
     6001 taaaaatgtg ccaaagataa aacatcggaa gaaaataaag aataaattag acaaagaaaa
     6061 agacaaagat gaaaaatact ctccaaaaaa ctgtaaactt cggcgcttgt ccaaaccacc
     6121 atttcagaca aatccatctc ctgaaatggt atccaaactg gatctcactg atgccaaaaa
     6181 ctctgatact gctcatatta agtccataga aattacttcg atccttaatg gacttcaagc
     6241 ttctgaaagt tctgctgaag acagtgagca ggaagatgag agaggtgctc aagacatgga
     6301 taataatggc aaagaggaat ctaagattga tcatttgacc aacaacagaa atgatcttat
     6361 ttcaaaggag gaacagaaca gttcatcttt gctagaagaa aacaaagttc atgcagattt
     6421 ggtaatatcc aaaccagtgt caaaatctcc agaaagatta aggaaagata tagaagtatt
     6481 atccgaagat actgattatg aagaagatga agtcacaaaa aagagaaagg atgtcaagaa
     6541 ggacacaaca gataaatctt caaaaccaca aataaaacgt ggtaaaagaa ggtattgcaa
     6601 tacagaagag tgtctaaaaa ctggatcacc tggcaaaaag gaagagaagg ccaagaacaa
     6661 agaatcactt tgcatggaaa acagtagcaa cagctcttca gatgaagatg aagaagaaac
     6721 aaaagcaaag atgacaccaa ctaagaaata caatggtttg gaggaaaaaa gaaaatctct
     6781 acggacaact ggtttctatt caggattttc agaagtggca gaaaaaagga ttaaactttt
     6841 aaataactct gatgaaagac ttcaaaacag cagggccaaa gatcgaaaag atgtctggtc
     6901 aagtattcag ggacagtggc ctaaaaaaac gctgaaagag cttttttcag actctgatac
     6961 tgaggctgca gcttccccac cgcatcctgc cccagaggag ggggtggcag aggagtcact
     7021 gcagactgtg gctgaagagg agagttgttc acccagtgta gaactagaaa aaccacctcc
     7081 agtcaatgtc gatagtaaac ccattgaaga aaaaacagta gaggtcaatg acagaaaagc
     7141 agaatttcca agtagtggca gtaattcagt gctaaatacc cctcctacta cacctgaatc
     7201 gccttcatca gtcactgtaa cagaaggcag ccggcagcag tcttctgtaa cagtatcaga
     7261 accactggct ccaaaccaag aagaggttcg aagtatcaag agtgaaactg atagcacaat
     7321 tgaggtggat agtgttgctg gggagctcca agacctccag tctgaaggga atagctcgcc
     7381 agcaggtttt gatgccagtg tgagctcaag cagtagtaat cagccagaac cagaacatcc
     7441 tgaaaaagcc tgtacaggtc agaaaagagt gaaagatgct cagggaggag gaagttcatc
     7501 aaaaaagcag aaaagaagcc ataaagcaac agtggtaaac aacaaaaaga agggaaaagg
     7561 cacaaatagt agtgatagtg aagaactttc agctggtgaa agtataacta agagtcagcc
     7621 agtcaaatca gtttccactg gaatgaagtc tcatagtacc aaatctcccg caaggacgca
     7681 gtctccagga aaatgtggaa agaatggtga taaggatcct gatctcaagg aacccagtaa
     7741 tcgattaccc aaagtttaca aatggagttt tcagatgtcg gacctggaaa atatgacaag
     7801 tgccgaacgc atcacaattc ttcaagaaaa acttcaagaa atcagaaaac attatctgtc
     7861 attaaaatct gaagtagctt ccattgatcg gaggagaaag cgtttaaaga agaaagagag
     7921 agaaagtgct gctacatcct catcctcctc ttcaccttca tccagttcca taacagctgc
     7981 tgttatgtta actttagctg aaccgtcaat gtccagcgca tcacaaaatg gaatgtcagt
     8041 tgagtgcagg tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     8101 tatatcttgt ggaaaggacg aagtttagag tattaaccgc atgtaacgcg ttaagtcgac
     8161 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     8221 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     8281 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     8341 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     8401 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8461 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8521 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     8581 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8641 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8701 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     8761 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8821 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     8881 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8941 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9001 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     9061 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     9121 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9181 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9241 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     9301 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9361 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9421 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     9481 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     9541 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     9601 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     9661 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     9721 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     9781 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     9841 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     9901 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     9961 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    10021 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
    10081 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    10141 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    10201 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    10261 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    10321 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    10381 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    10441 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    10501 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    10561 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    10621 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    10681 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    10741 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    10801 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    10861 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    10921 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    10981 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    11041 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    11101 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    11161 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    11221 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    11281 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    11341 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    11401 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    11461 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    11521 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    11581 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    11641 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    11701 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    11761 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    11821 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    11881 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    11941 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    12001 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    12061 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    12121 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    12181 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    12241 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    12301 aagc
//
