LOCUS       sequence-396301-        6841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains WRKY40 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP42
FEATURES             Location/Qualifiers
     source          1..6841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6427..6446)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6428..6446)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6456..6478)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6672..6691)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6791..6813
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc ttgtgtataa ctattatgca gcctttttca
     2281 acaaccaatg taaaaacgaa gtactaacta ctaaattagg agtaggagaa aagtaaaaca
     2341 attcgcaaga atggaatcta aaatttggga ttagtctttg attttcacac ttggtcataa
     2401 aaattaagtg gacatggaca ttgaaaacga aatccaaaag accatttcga aaaagaggca
     2461 agaaattata taataattaa tatttaatag acaacacatg ttttagactt ttactagttc
     2521 atgaatcgta cggccttttt tgttccttta actaagttca atgcatcctc tagtagacac
     2581 ctactgctta actaaccaac ataaaaaaat atgattaatt ctccccgaat agtattgttg
     2641 tgttactttt acacaaattg aaaaaaaagt aaacgacagt tattagttat tttaaaacga
     2701 atgaaacatt atgtaaaaat atgtcaaatt aatatgctta gttaattaga gaaagtcaag
     2761 actttgcaaa aatgcatcca aaattaaaca aatgtatacc cgaaagttcg agtgtgaatt
     2821 agttctgtca ctaaaatatt ccgtttaaac tcaagtctta taaaatgtac ggatattata
     2881 ccttttgttt tctcgagctt attatacctt aaaaatgtaa attggatgag gatattgagg
     2941 aaaaaaataa gacttggaaa gaaatattag taagctttta ttaaaaaaaa aaaaagaaga
     3001 gataattcaa gacctgaaga agacaggcac gaagtagcta ggggagggaa tggggaagta
     3061 aaaccaaacc actcgtgcgt tgtgtgttag gattttcccg agtcaacgag gtcaacggtc
     3121 aaagaggtca aagtctttaa aatacaggaa aggaattgtg ctttcgttac ttgctttctt
     3181 acgttctttc tgtcctttgg aggagaatag ttttgttttt gcttttgttg atttttctgt
     3241 ccactttgta tatatttttt tttttttatg acttcatcac ctgtgacgtc taataattcc
     3301 atcaccgact gcccatttct tttcttttta ttgtctctac aatttcgatt atattttttg
     3361 gtcggcagcc agtctcttcg ttcttctttt ttttctctgg acgtccattc ccaacatgca
     3421 tagtgtttga cacaacaata tatgacagct aacttttaag tataattcga aatgaactaa
     3481 atcgttgatt atacataagt ttaggttatg agtttttgtc ttttgcaatc tattaattaa
     3541 caattggttt gtgaccaaat tgttcgttga gtgttgtaag aaccaaccta tttcaatcag
     3601 tttcttaaaa aaggaaaggg aaggcaaaaa aaaaaagaac tattagcagc cttacttttc
     3661 tatgataata ttagtcagta gggaaatata tatatatata tatatatata tatatatata
     3721 tatatatata taatttggac cgacgactga ttacaataac tgttaaagct taaaccggcg
     3781 gaacttaatc ttatataatt aatatgattg taattagctg tatgataatt ttatgttgtt
     3841 aatgatgatg tattaccttt tcgctatagg gtttatagat tacatctata cttctgcata
     3901 caattagata ttaataaagt tataaaaaca aattaaactg aataattgtg tgtgtgttac
     3961 gttgcatgtg ttattagtta tggagaaata tgatttacgt cttctgaaat gctatcaacg
     4021 attggtttca gatttaaaga aactatctga ccaaaatcct gagatatctt aattagatat
     4081 atctatttaa actttcaagt tgttatatgt taaacagtaa atggcgtttt ccgtattact
     4141 catctagtag atcgtagtaa gttatatacg tttttacaat gaaaggtttc ggcaatgtaa
     4201 gttgtatacg tggtacgagt gagtatccat acttctaccg gaaagaatag agatcaagaa
     4261 aaatgaatat aattaataaa gctctaccga ggaaaagaag agtgaaggat aatggagaaa
     4321 aataagaaaa aagatgatga gatctgaatt aagattttat tattcacaat tacaagaata
     4381 ggtacagtcc tggtttgtgc taaaccaaat tcgaatatca aatataaatt acttataggt
     4441 tcgactgtaa aatgagatat gaaagaaaga aataatatat gatgatgtaa tgcatgagaa
     4501 agtccaaaat gatcggcata tgggattaag cgattccata tagttaagtc aagttactat
     4561 ttcagataaa tggataagtt tgtcgtctat atgttctgtt atctccaaac ccaaatctat
     4621 aacattgcga catttttgaa tcgttaacgg caaaataagt ggggaggggg ggggaaggaa
     4681 aaaaggaagg acactgagat tttgataaag ttcggtagcc aaatttgtgc atgactttta
     4741 agaaaatacg tatgctgatc caaaaacaaa caactaaata gattttagtt tgagaaaatc
     4801 gaagtaacaa aattattatt taatatatag tgtgagaatt taacagctac taataattat
     4861 cataacacga attaagtgta atagtaatat atgtaggaaa atggtttagc accactcact
     4921 tgattaactc gacttaaacc aaatataaag taatatttat agatgtagtt ggttgtattt
     4981 ttgcaagctt tggtactcga tatctaaatt tttaatggaa aaattagtcg agtggtaatt
     5041 cgttatatat aagaaaatat tttgtttgta attgtgcaaa ctttagcatt ggacatctaa
     5101 ctatattctg aaaaatcagc ggaatgataa aagttcggat tttccttctt tttatttaag
     5161 taaacataat tggtgttctg aaaaccaaaa gaaggaagaa ggcaattaag atttgttaaa
     5221 gttcgtcacg atattgatgt caagaaaaaa ggaaaaaaaa aaaaaatcgt cacgatggta
     5281 tcgtcaattt gtctagtgga ttttgaaaag ccgtgtgggc ttgactttga ccgaacaagg
     5341 cacacgtata catctagtac tactgaagaa atctctcatt agaacgcaaa ccacgttccg
     5401 cacactctta atatactatt agtctattac tatcaccaac tagttgtcat tttttttgtc
     5461 aaacgtgcta attaattcac aaatagaagt attaaacttc ctaattaagc acgcctttac
     5521 tacatttcat tattattata catgtaatca accatttctt ttatcccaca tgtcatttgt
     5581 agtcctgatt ttatgacttt atgcatcaag ttatcttttt ttttgtcact ccatgcatca
     5641 agttatctaa acaacataat tattgtctga aaaatctaag atgatataca acatatgtat
     5701 ttaattacac attaggcttg tttaacaatt cttgattatt tttactcttt ttgggtgagt
     5761 tcttgatgga tgtcttgaat tacaaaacta catattctga aaatattaac cttgagttta
     5821 ggccggctat gctataacga aaaataaata aaagcaacta acccgacaga aatgtcaacc
     5881 aactatccaa aatgaaaaac aaacgtggag agcgtcatat tattagtagg ccaatcacac
     5941 accaaatgtt tgtttaacaa aaaaaataag aatgtcaacc aactatccaa aattaaaatc
     6001 aagaatacat ttcatatgtt tccaatatgt ttttcttaaa caaaattggg actaaagatt
     6061 ggaccaaata cgtgtgcggg tggacccgat agaagccgag ttgagtctaa agactaaaaa
     6121 gtaaacaaaa aatgataaga ccatcactta gtttattgca tgaaaatgaa aagcacatgc
     6181 tcctctctct ctcttctgcc tctttccatc tagtttcctc ttaaaactcc attatatata
     6241 gaaacaaaat cttttctctc ttcaacaatc ccaccattaa aaaagaagaa cccaagatcg
     6301 actcttactt cgaatctctc tcaactttct tcctcagctt acgggaactt ccacacatat
     6361 acatccacaa gaacccatat cgaagattca tcctacatat atttacaaca tgagaccgaa
     6421 ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa
     6481 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     6541 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     6601 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     6661 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     6721 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     6781 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     6841 a
//
