LOCUS       sequence-396303-        5367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains ERF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP54
FEATURES             Location/Qualifiers
     source          1..5367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4953..4972)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4954..4972)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4982..5004)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5198..5217)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5317..5339
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tctctcccaa ttgatatttt tgttatttct
     2281 tagatagaat tgatatcatg actcaagttt taattttttc attcactcga aaaaaaaatt
     2341 gatttgagtc cagaaattct tttgtttcaa atccaaatgg attttccgaa cgacagaaaa
     2401 tgttgccgat tatacataaa taatttaaga aaatcatgta aatacattaa atttttttaa
     2461 ctgatatgac tataaaatca taaaaatgac tagaaaatat acaaataata tgaccacgta
     2521 ccatgaatgt gtagctatcg ccttacgcct aacaccttag tcaaatttga atgcttactg
     2581 gactttgttt tctttctttt ttgatttgac tataaataat ctttttatat ttgcctattt
     2641 ccatataaaa atttgaatca ctaagaattg aaattctgga gcttccactg accgaaggga
     2701 aaatattgag gaatataaat cattttagaa aacttgttta tttttatatg aagtattagt
     2761 aattattcga ataattatat tattattttt tgtgtcaatt tatggaaatc atttagcaac
     2821 tgatatcaaa agcaattatt cttgggttac ttttctgatg tcaatattga aatcaattaa
     2881 tttattcatt ttcggtacgt gtaagaagtt taaagtattt taccacaaaa aaatgaagtt
     2941 taaagtaaag ccaaaagaga tacaatgtcc aggtttgaag tttaaaatag agccaaaaac
     3001 tttgaacacg tgcgggatat caaataatca gttatgcata gttttggatg ggcttaagaa
     3061 agaataaata attttgtgtc ctactggact caggatgcat gtgatgatgt gtagtgttct
     3121 aatagaattt agttgcgtat ccgttcgaat aattattttc aacacctcct acttttgtta
     3181 cattgtttta aatgactatt gacaaaataa aagtaaatgt aaataactaa aacagaaaag
     3241 atgtgagaaa aactaggaaa ttcttttttt tgaagaatta ttaaatttgc tagatttgga
     3301 taatcgattc aacatttgga aaagaaaaaa aatatctaag gatggttgaa agatagttta
     3361 tgatcaaact agccaggatt ttagaggggt catcagtttt ctgaacattt atatgttgtt
     3421 ttccgataat actataaatt tgatcaacta attagatgac ttaaaataat gtaattggga
     3481 taatgtttaa gaaaatatta ggtaactcat ataatttaag agttataatt tggttataat
     3541 ttgcaccaaa tttgaaatat atttctacta taagataact agaaatagtg aagaaaaaaa
     3601 caaaggaaac tagaaaaaat gaaaaaatgg cacatgaagt atcttttttg tttgagaaag
     3661 aagttgaaag cagataaagc caaattatct tctaaacatg agatggctcg aaattatttg
     3721 ttgtttggga ttcaaggggg catgtatctt gaatccttcc aaccaacaat tttctttaac
     3781 catgcttttg gccaaaagat tttctttaat aataattaat aatagcttcc taagattata
     3841 ggataatgat ttgatatttt atgtaatcat atttgcaata cctaagcatg aaaatgtttt
     3901 gttgagttga tccagcagtt attttggaat atggcttaat cattttcaca tcgatcttgt
     3961 tatttctgtt cgtgaaatgt gaactttcgc agttatttgt tttattcata agttgtttta
     4021 gatcaaataa gtggagtcca cttagaaatt ataagatggg aattttattt gcatatcaaa
     4081 attatctata aaaattttag tcaactgatt tttagttttc tactaaaatc gccatcttat
     4141 tttcttctag attcaatttg atctgaatgt ggcatgtagg ttgataaaac ttatcaaata
     4201 gcatcaaatc agtattttct aaaacttttt acatcatcat aaatctttca cttataaaat
     4261 ataatatctt gggtacacca aagtaatcct gatatatact acaaaatata acattttagt
     4321 tgttttcata tgtggttaat cttttttagg tcgtacatga ttcataaacc ttaccgtcat
     4381 aaaaagaaaa ataaattata tttaaactaa gaaatgtgat atgatgcaaa agtgacaaaa
     4441 tttcgatgaa tcatgtgatg ataatacgaa atgatatacc taacctacac tgctcattct
     4501 acatttgaag aatatatacc taaatataca taccaattct gggataatta cataatatac
     4561 agtttcatat tagtctcacg taattacgat tcacgacagt aagaagatta gtttaaacgg
     4621 tacaaaacaa ttcatatatt acgaaaacat taaatatcac acgaagagaa gaaatatgca
     4681 aaacgtcttt gctcgaatct tcgacgaagc aacatagttt ttcatttgag tgtaacatta
     4741 tttttcgtaa attcccattt caccctttta ttacatgaca tttgtctttc actgagacag
     4801 taaaaactca agtctaattt ggacatttgc atatacatac tcattttttt ctctatatat
     4861 atagaccatg tcaatccact aacgatccct aaccgaaaac agagtagtca agaaacagag
     4921 tattttttct acaacatgag accgaattct cgccctatag tgagtcgtat tacctggccg
     4981 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     5041 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     5101 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     5161 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     5221 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     5281 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     5341 tttcaccgtc atcaccgaaa cgcgcga
//
