LOCUS       sequence-396308-        9944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PB plasmid expressing Halo-tagged PP7 coat protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   De Pace R, Ghosh S, Ryan VH, Sohn M, Jarnik M, Rezvan Sangsari P, 
            Morgan NY, Dale RK, Ward ME, Bonifacino JS
  TITLE     Messenger RNA transport on lysosomal vesicles maintains axonal 
            mitochondrial homeostasis and prevents axonal degeneration.
  JOURNAL   Nat Neurosci. 2024 Apr 10. doi: 10.1038/s41593-024-01619-1.
  PUBMED    38600167
REFERENCE   2  (bases 1 to 9944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2024 Apr 10. doi: 10.1038/s41593-024-01619-1."
COMMENT     Sequence Label: PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE
FEATURES             Location/Qualifiers
     source          1..9944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(41..60)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     371..393
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     385..402
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     386..402
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   532..594
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        879..1090
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          1091..2029
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1986..2006
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2054..2074
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2924..3814
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             3860..4021
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             4046..4933
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             4979..5140
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             5198..6088
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     protein_bind    complement(6111..6144)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6200..6788
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6253..6273)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6671..6682)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6791..6807)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6792..6808)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     repeat_region   7211..7245
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7736..7752)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7736..7752)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7749..7771)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7760..7776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7784..7790,7791..7808,7809..7814))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7829..7850
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7967..7984)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8186..8774)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8266..8285)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8945..9736,9737..9805))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9568..9587
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9806..9910)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
       61 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
      121 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
      181 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
      241 tattcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
      301 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      361 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt
      421 cacgtttttg aacccgtgga ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg
      481 atggagcaga tgaagatgct cgacacgctg cagaacacgc agctagatta accctagaaa
      541 gataatcata ttgtgacgta cgttaaagat aatcatgtgt aaaattgacg catgtgtttt
      601 atcggtctgt atatcgaggt ttatttatta atttgaatag atattaagtt ttattatatt
      661 tacacttaca tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta
      721 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttatg ggcccgctct
      781 agagaacctg aatgcagtct ctcggagggc gaagaatcct gcaggctcga ggcggccgcg
      841 tggataaccg tattaccgcc atgcatgtgc ccgtcagtgg gcagagcgca catcgcccac
      901 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
      961 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     1021 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     1081 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     1141 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     1201 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     1261 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     1321 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     1381 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     1441 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     1501 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     1561 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     1621 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     1681 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     1741 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     1801 gccgtccagg cacctcgatt agttctcgcg cttttggagt acgtcgtctt taggttgggg
     1861 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     1921 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     1981 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagc
     2041 tagcgccacc atgcccaaga agaagaggaa ggtgagcaag acaatcgtgc tgtccgtggg
     2101 cgaggccaca agaacactga cagagatcca gagcaccgcc gatagacaga tctttgagga
     2161 gaaggtgggc cccctggtgg gcaggctgag actgaccgcc agcctgaggc agaatggcgc
     2221 caagaccgcc tacagagtga acctgaagct ggatcaggcc gatgtggtgg actgctccac
     2281 ctccgtgtgt ggcgagctgc ctaaggtgag atacacacag gtgtggtccc acgacgtgac
     2341 catcgtggcc aattccaccg aggccagcag aaagtccctg tacgacctga ccaagtccct
     2401 ggtggccaca agccaggtgg aggacctggt ggtgaacctg gtgcctctgg gcagaagggc
     2461 cgatcctctg gccagctgcg gcagaagcaa gacaattgtg ctgagcgtgg gcgaggctac
     2521 aaggaccctg acagagattc agagcaccgc tgacaggcag atctttgaag agaaggtggg
     2581 acccctggtg ggaaggctga ggctgaccgc ctccctgaga cagaacggcg ccaagacagc
     2641 ctacagggtg aatctgaagc tggaccaggc cgacgtggtg gactgttcca catccgtgtg
     2701 tggagagctg cctaaagtga gatacaccca ggtgtggagc cacgacgtga caatcgtggc
     2761 caactccacc gaggcttcca gaaagagcct gtacgacctc acaaagtccc tggtcgccac
     2821 ctcccaggtg gaggatctgg tggtgaatct ggtgcctctc ggcaggagag ccgaccccct
     2881 ggcttccacc agggactcca ccagcggcgg ctccggagga ggaatggccg agatcggcac
     2941 aggcttcccc ttcgatcctc actacgtgga ggtgctgggc gagagaatgc actacgtgga
     3001 tgtgggcccc agggacggca cccctgtgct gttcctgcac ggcaacccca catccagcta
     3061 cgtgtggagg aatatcatcc cccacgtggc ccctacacac agatgtattg cccccgatct
     3121 gatcggcatg ggcaagtccg ataagcccga cctgggctac ttcttcgacg atcacgtgag
     3181 attcatggat gccttcatcg aggccctggg cctggaggag gtggtgctgg tcattcacga
     3241 ctggggctcc gccctgggct tccactgggc taagagaaac cccgagagag tgaagggcat
     3301 cgccttcatg gagtttatca ggcccatccc tacatgggac gagtggcctg agttcgccag
     3361 ggagacattc caggccttta ggacaacaga cgtgggcagg aagctgatca tcgaccagaa
     3421 cgtgtttatc gagggcaccc tgcctatggg cgtggtgagg cctctgacag aggtggagat
     3481 ggatcactac agggagccct ttctgaaccc cgtggacagg gagcctctgt ggagattccc
     3541 taacgagctg cctatcgccg gcgagcctgc caatatcgtg gccctggtgg aggagtacat
     3601 ggattggctg caccagtccc ccgtgcccaa gctgctgttt tggggcaccc ccggcgtgct
     3661 gatccctcca gctgaggccg ccaggctggc caagagcctg ccaaactgca aggccgtgga
     3721 catcggccct ggcctgaacc tgctgcagga ggacaacccc gatctgattg gctccgagat
     3781 cgccagatgg ctgagcaccc tggagatctc cggccacggc atggacgagc tgtacaaggg
     3841 cggcggcaga acagaggagt acaagctgat cctgaacggc aagaccctga agggcgagac
     3901 aacaacagag gccgtggatg ccgccacagc cgagaaggtg tttaagcagt acgccaatga
     3961 taacggcgtg gatggcgagt ggacctacga cgacgccaca aagacattca ccgtgaccga
     4021 ggcctccggc ggctccggcg gaggagctga gatcggaaca ggctttccct tcgaccccca
     4081 ctacgtggaa gtgctgggcg aaaggatgca ctacgtcgat gtgggcccaa gagacggcac
     4141 acctgtgctg tttctgcacg gcaatcccac aagctcctac gtgtggcgga atatcatccc
     4201 acacgtggcc cccacccaca gatgtatcgc ccccgacctg atcggcatgg gaaagagcga
     4261 caagcccgat ctgggctact ttttcgatga ccacgtgagg ttcatggacg cctttatcga
     4321 ggccctcggc ctggaggaag tggtgctggt cattcatgac tggggcagcg ccctgggctt
     4381 tcactgggcc aagaggaatc ctgagagagt gaaaggcatc gcctttatgg agttcatcag
     4441 acccatcccc acatgggacg aatggcctga gtttgccagg gagacttttc aggccttcag
     4501 gaccacagat gtgggcagga aactgatcat cgatcagaat gtgttcatcg agggcacact
     4561 gcccatgggc gtggtcaggc ctctgaccga ggtggagatg gaccactaca gagagccttt
     4621 cctgaacccc gtcgacaggg agcccctgtg gaggttccct aatgagctgc ccatcgccgg
     4681 cgaacccgcc aacatcgtgg ccctcgtgga ggagtatatg gactggctgc accaatcccc
     4741 cgtgccaaag ctgctgttct ggggcacccc aggcgtgctg attccccccg ctgaggccgc
     4801 tagactggcc aagtccctgc ctaattgtaa ggccgtggat atcggccccg gcctgaatct
     4861 gctgcaggaa gataatcctg atctgatcgg aagcgagatc gcccggtggc tgtccaccct
     4921 ggagatttcc ggccacggaa tggatgagct gtacaaaggc ggcggcagga cagaggagta
     4981 taagctgatc ctcaacggca agacactgaa gggcgaaaca accaccgagg ccgtggacgc
     5041 cgccaccgct gaaaaggtgt ttaagcaata cgccaacgac aacggcgtgg acggcgagtg
     5101 gacatacgat gatgccacaa agacctttac cgtgacagag gccagcggcg gcggcggatc
     5161 tggaggagga agcagcctgc ccgtggccga gttcggcatg gccgagattg gcaccggctt
     5221 cccctttgac cctcactacg tcgaggtgct gggagagagg atgcactatg tggacgtggg
     5281 ccccagagac ggcaccccag tgctgttcct ccacggcaat cctacatcct cctacgtgtg
     5341 gagaaatatc atccctcacg tggccccaac acacagatgt attgctcctg acctgatcgg
     5401 aatgggcaag agcgacaaac ccgatctggg atacttcttc gatgaccatg tgaggttcat
     5461 ggatgctttc atcgaggctc tgggcctgga agaggtggtg ctcgtgatcc acgactgggg
     5521 aagcgccctg ggatttcact gggctaaaag gaaccccgag cgggtgaagg gcattgcctt
     5581 catggagttt attagaccca tccctacctg ggacgagtgg ccagagttcg cccgggagac
     5641 atttcaggcc tttagaacca cagatgtcgg caggaagctc atcatcgatc aaaatgtgtt
     5701 tatcgaaggc accctgccaa tgggcgtggt tagacctctg acagaagtgg agatggatca
     5761 ttacagggag cctttcctca accccgtgga tagagagcct ctgtggaggt ttcctaatga
     5821 gctccccatc gccggagagc ctgccaacat cgtcgccctg gtggaagagt acatggactg
     5881 gctccaccag agccccgtgc ccaaactgct gttttgggga acccctggcg tgctgatacc
     5941 tcctgccgag gccgccagac tggccaaaag cctgcccaac tgcaaggctg tggacatcgg
     6001 acccggcctg aacctcctgc aggaggataa tcctgacctg attggcagcg agatcgctag
     6061 gtggctgagc acactggaga tcagcggctg agaattcgtc gagggaccta ataacttcgt
     6121 atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact aggtacaatt
     6181 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6241 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6301 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6361 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6421 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6481 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6541 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6601 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6661 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6721 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6781 ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa
     6841 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     6901 aggtgggttt tccagtcaca cctcaggtac ctttaaggat tctataacaa gaaaatatat
     6961 atataataag ttatcacgta agtagaacat gaaataacaa tataattatc gtatgagtta
     7021 aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg gtcgttatag
     7081 ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct ccaagcggcg
     7141 actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag agagcaatat
     7201 ttcaagaatg catgcgtcaa ttttacgcag actatctttc tagggttaat ctagctgcat
     7261 caggatcata tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg gcgctatctg
     7321 ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag
     7381 tttgccgagg atgactgctg ctgcattgac gttgagcgaa aacgcacgtt taccatgatg
     7441 attcgggaag gtgtggccat gcacgccttt aacggtgaac tgttcgttca ggccacctgg
     7501 gataccagtt cgtcgcggct tttccggaca cagttccgga tggtcagccc gaagcgcatc
     7561 agcaacccga acaataccgg cgacagccgg aactgccgtg ccggtgtgca gattaatgac
     7621 agcggtgcgg cgctgggata ttacgtcagc gaggacgggt atcctggctg gatgccgcag
     7681 aaatggacat ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat
     7741 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7801 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7861 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7921 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7981 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8041 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8101 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttgga tccggccgcg ttgctggcgt
     8161 tggatccggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8221 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8281 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8341 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8401 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8461 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8521 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8581 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8641 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8701 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8761 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8821 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8881 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8941 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9001 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9061 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9121 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9181 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9241 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9301 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9361 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9421 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9481 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9541 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9601 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9661 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9721 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9781 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9841 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9901 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgac
//
