LOCUS       sequence-39631-e         585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1360
FEATURES             Location/Qualifiers
     source          1..585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             249..260
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 aaataggcgt atcacgaggc cctttcgtct tcaagaatta attccgatca tattcaataa
       61 cccttaatga attcgagctc ctaggccggc cagggagagg tgccgtggtg agactgaccc
      121 tcgggcccac gggtggatga tggcaaaggt cctgtggcag gtgtgggaga agcagggctg
      181 cggccagctg ccctgagagg cttcaaattc aggccagatc tgctctgggc aagaggaagc
      241 tgtgggtttg ggagcttcag agacagggga gcaggcctgt cactcaccct cttttcctta
      301 aaggctgtca cccccagcag tgcaccctgc agcctgccta tcccctgtgc agctccagct
      361 ccgtctgtcc ccagcaatgc accccacgcc catgtccccc actccctatg atgctctcgc
      421 gccttctgga gcagcagtgg taccagctgg aggctgtgga aaaattgaga ggagacaaaa
      481 gtgggaagag caggccgtgg agagagtgtc taaacacagg gactatttgg agattgtctt
      541 atttttggtt tgaaatgggc ccacctgcta gtattgtaca aacgg
//
