LOCUS       sequence-396312-        9887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible lentiviral expression of Eomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Xie G, Lee JE, Senft AD, Park YK, Jang Y, Chakraborty S, Thompson 
            JJ, McKernan K, Liu C, Macfarlan TS, Rocha PP, Peng W, Ge K
  TITLE     MLL3/MLL4 methyltransferase activities control early embryonic 
            development and embryonic stem cell differentiation in a 
            lineage-selective manner.
  JOURNAL   Nat Genet. 2023 Apr 3. doi: 10.1038/s41588-023-01356-4.
  PUBMED    37012455
REFERENCE   2  (bases 1 to 9887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2023 Apr 3. doi: 10.1038/s41588-023-01356-4."
COMMENT     Sequence Label: pCW57.1-Eomes-3xT7
FEATURES             Location/Qualifiers
     source          1..9887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2805..2922
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2976..3290
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2984..3002
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3020..3038
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3055..3073
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3091..3109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3127..3145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3162..3180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3198..3216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3263..3287
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        5593..6103
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6022..6040
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6113..6709
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6113..6132)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6609..6629
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6710..6763
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6764..7381,7382..7387,7388..7423,7424..7426,7427..7462,
                     7463..7465,7466..7501,7502..7510)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7550..7570)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7968..7979)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8184..8417
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8489..8610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8526..8545)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8580..8599
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8650..8785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8785..8856
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8857..8925,8926..9717)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9075..9094)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gttttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2821 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2881 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2941 agcagagatc cactttggcc gcgaatcgat atgtcgagtt tactccctat cagtgataga
     3001 gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
     3061 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     3121 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
     3181 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
     3241 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagaattg
     3301 gaattccgcg aacgcgtgcc accatgcagt tgggagagca gctcctggtg agctcggtga
     3361 acctgcccgg cgcgcacttc tactcgctgg agagtgctcg cggaggagga ggaggaggag
     3421 gcggaggagg agggggaggc ggagggagcg tcagcctcct ccccggtgct gccccctcgc
     3481 cccagaggct ggacttagac aaagcgtcca agaagtttcc gggcagtctc ccgtgccagg
     3541 cggggagcgc agaacccgca ggcgccggcg cgggggcccc cgcggccatg ctcagtgacg
     3601 cggacgctgg ggacaccttc gggagcacct cggcggtggc caagcccggg cccccggacg
     3661 gccgcaaggg ctccccgtgc gcggaggagg agctgccctc cgccgccacc gccgcggcca
     3721 ccgcgcgcta ctccatggac agcctgagct ccgagcgcta ctacctcccg tcgccgggac
     3781 cgcagggctc cgagctcgcc gcgccctgct cgctcttcca gtacccggcg gcggccggag
     3841 cagcccacgg acccgtgtac cccgcgtcca atggcgcgcg ctacccctac ggctccatgc
     3901 tgccccccgg tggattcccc gccgccgtgt gcccgcccgc gagggcgcag ttcggccccg
     3961 ctgcgggttc ggggagcggc gctggtagca gcggcggtgg tgccggcggt cctggcgcct
     4021 atccctacgg ccagggttct ccgctctacg ggccatacgc cggaacctca gcggccgggt
     4081 cttgtggagg attggggggc ctaggggtgc ctggctccgg cttccgcgcc cacgtctacc
     4141 tgtgcaaccg gcccctatgg ctcaaattcc accggcacca aactgagatg atcatcacca
     4201 aacagggcag gcgcatgttt cctttcttga gcttcaacat aaacggactc aaccccaccg
     4261 cccactacaa tgttttcgtg gaagtggttc tggccgaccc taaccactgg cgcttccagg
     4321 ggggcaagtg ggtgacctgc ggcaaagcgg acaataacat gcagggcaat aagatgtacg
     4381 ttcacccaga atctcctaac actggctccc actggatgag gcaggagatt tcctttggga
     4441 agttaaaact caccaataac aaaggtgcaa acaacaacaa cacacagatg atagtgttgc
     4501 agtctctgca caaataccaa ccgaggctgc acatcgtgga agtgacagag gacggtgtgg
     4561 aggacttgaa tgaaccttcc aagactcaga ccttcacctt ctcagagaca cagttcatcg
     4621 ctgtgacggc ctaccaaaac acggatatca cccagctaaa gatcgaccat aaccccttcg
     4681 ccaaaggctt ccgggacaac tacgattcca tgtacacggc ttcagaaaat gacaggttaa
     4741 ctccatctcc cacggattcc cctagatccc atcagattgt ccctggaggt cggtacggcg
     4801 ttcaaaactt cttcccggag ccctttgtca acactttgcc tcaagcccga tattataacg
     4861 gtgagagaac cgtgccacag accaacggcc tcctctcacc ccaacagagc gaagaggtgg
     4921 ccaaccctcc ccagcggtgg cttgtcacgc ctgtccagca acctgtgacc aacaagctag
     4981 acatcggttc ttatgaatct gaatatactt ccagtacctt gctcccatat ggtattaagt
     5041 ccttgcccct gcagacatcc catgccctgg ggtattaccc tgacccgacc ttccctgcta
     5101 tggcagggtg gggaggccgt ggcgcttatc agaggaagat ggcagctgga ctaccatgga
     5161 catccagaat gagcccacct gtcttcccag aagatcagct tgccaaggaa aaagttaaag
     5221 aagagattag ttcctcctgg atagagactc ccccctccat caagtctcta gactccagcg
     5281 actccggggt gtacaacagc gcttgcaaga gaaagcgcct gtctcccagc acccccagca
     5341 atggaaactc gccccccata aagtgtgagg acattaacac tgaagagtac agtaaagaca
     5401 cctccaaagg catgggggct tattatgctt tttacacaag tcccggatcc ggtggaagta
     5461 tggctagcat gactggcggg cagcaaatgg gcgggtctag cgcaatggca tctatgaccg
     5521 gaggtcaaca gatgggaggt tcctcagcca tggcgagtat gacaggtggc caacagatgg
     5581 gttgagttaa ccggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg
     5641 acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac
     5701 attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc
     5761 ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg
     5821 gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg
     5881 agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg
     5941 cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg
     6001 ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc
     6061 agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagcaattca ccatgaccga
     6121 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     6181 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     6241 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6301 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6361 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6421 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6481 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6541 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6601 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6661 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgccg aaggtagagg
     6721 ttctctcctc acttgtggtg atgttgaaga aaaccctggt ccaatgtcta gactggacaa
     6781 gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac
     6841 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     6901 gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca
     6961 cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata
     7021 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     7081 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     7141 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     7201 ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     7261 gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt
     7321 ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg
     7381 cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga
     7441 cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct
     7501 ccccgggtaa ctaagtaagg atcgatccaa gatatcgtat tcttaactat gttgctcctt
     7561 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7621 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7681 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7741 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7801 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7861 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     7921 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7981 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     8041 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgtt tcgcctcggc
     8101 gtccggacta gaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     8161 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     8221 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8281 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8341 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8401 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     8461 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     8521 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8581 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     8641 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8701 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8761 tagtgaggag gcttttttgg aggcctttca aatatgtatc cgctcatgag acaataaccc
     8821 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8881 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8941 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9001 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9061 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     9121 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9181 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9241 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9301 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9361 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9421 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9481 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9541 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9601 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9661 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9721 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9781 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9841 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
