LOCUS       sequence-396314-        5692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The ubiquitous eft-3 promoter drives overexpression of the mutant 
            act-5(ptc) gene including introns..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Welker JM, Serobyan V, Zaker Esfahani E, Stainier DYR
  TITLE     Partial sequence identity in a 25-nucleotide long element is 
            sufficient for transcriptional adaptation in the Caenorhabditis 
            elegans act-5/act-3 model.
  JOURNAL   PLoS Genet. 2023 Jun 29;19(6):e1010806. doi: 
            10.1371/journal.pgen.1010806. eCollection 2023 Jun.
  PUBMED    37384903
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2023-06-29"; volume: "19(6):e1010806. doi"; pages: "
            10.1371/journal.pgen.1010806. eCollection 2023 Jun"
COMMENT     Sequence Label: eft-3p:act-5(ptc):tbb2 3' UTR
FEATURES             Location/Qualifiers
     source          1..5692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..600
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     terminator      2388..2711
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     3388..3410
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3448..3466)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3534..3638
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3639..3707,3708..4499)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3857..3876)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4670..5258
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5159..5178
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcacctttgg tcttttattg tcaacttcca ttggttcttc cattgtttct gttaaattaa
       61 tgaatttttc ataaaataaa gacattatac aatataaaga tgaagaattt attgaaaata
      121 aactgccaga gagaaaaagt atgcaacact cccgccgaga gtgtttgaaa tggtgtacgg
      181 tacattttcg tgctaggagt tagatgtgca ggcagcaacg agagggggag agattttttt
      241 gggccttgtg aaattaacgt gagttttctg gtcatctgac taatcatgtt ggttttttgt
      301 tggtttattt tgtttttatc tttgttttta tccagattag gaaatttaaa ttttatgaat
      361 ttataatgag gtcaaacatt cagtcccagc gtttttcctg ttctcactgt ttagtcgaat
      421 ttttatttta ggctttcaac aaatgttcta actgtcttat ttgtgacctc actttttata
      481 tttttttaat ttttaaaaat attagaagtt tctaggataa ttttttcgac ttttattctc
      541 tctaccgtcc gcactcttct tacttttaaa ttaaattgtt tttttttcag ttgggaaaca
      601 gttaacgatg gaagaagaaa tcgccgccct cgttgtcgac aatggatccg gaatgtgcaa
      661 ggccggattc gccggagacg atgctccacg cgccgtcttc ccgtccatcg tcggacgccc
      721 acgtcatcag ggagtgatgg tcggtatggg acagaaggac tcgtatgtcg gagatgaagc
      781 acaatccaag agaggaatcc tcaccttgaa gtacccaatc gagcacggaa tcgtcaccaa
      841 ctgagatgat atggagaaaa tctggcatca cacattctac aacgagcttc gtatcgcccc
      901 agaggaacac ccagttctcc ttaccgaggc tccattgaac ccaaagagca acagagaaaa
      961 gatgacacag atcatgtttg agaccttcaa cactccagcc atgtacgtca acattcaggc
     1021 tgtgctttcc ttgtacgctt ccggaagaac caccggaatc gttttggaca ccggagatgg
     1081 tgtcactcac accgttccaa tctatgaagt aggtagatgg gtttagagaa tataagaaga
     1141 cagcaatctt ctttgacttg gcggtgtaaa gttttggaga ttacgtctgc ttcgactaat
     1201 ttttgcagcc agaaacaggc tcacagcgct ttcggaaact tttagttcag ctcagcagct
     1261 cttatcctta gttttaagtt tttggaactg taagaactta gtaagagcaa ggccacatca
     1321 tttctctgca aatcatagcc tgagcttctg atcttcttat ctgatccaca atcttcttgt
     1381 gagaattctt tgggatctaa gaaatctaga acctacatcc gatcttatct tctaatcttc
     1441 cagggatatg ccctcccaca tgccatccaa cgtctcgatc ttgccggacg cgacttgacc
     1501 gactacatga tgaagatcct caccgagcgc ggttacactt ttaccaccac cgccgaacgc
     1561 gaaatcgtcc gggacatcaa agagaagctt tgctacgtcg cccacgactt cgagtccgag
     1621 ctcgccgccg ccgcctcctc ctcctccctc gagaagagct acgagcttcc cgacggacag
     1681 gttatcacca tcggaaacga gagattccgc tgcccagaag tcctcttcca gcctgccttc
     1741 atcggaatgg aaggtgccgg aatccacgag accacctacc aatccatcat gaagtgcgat
     1801 gtcgacatca gaaaggatct ctacgccaac actgttctct ccggaggtac ctccatgttc
     1861 ccaggaatcg ctgatcgtat gcaaaaggag attcaacatt tggctccaag gtaagcttca
     1921 catttctcag tacatgagat tataattctt gtattttcag cacaatgaag atcaagatta
     1981 ttgctccacc agagcgcaag tactccgtct ggatcggagg atctattctt gcttccctct
     2041 caaccttcca gcaaatgtgg atctccaagc aggagtacga cgagtccggc ccatcgattg
     2101 ttcaccgaaa gtgcttctaa gctgattttt ttttcaaatt ttttttttca acatgtgcct
     2161 tccatttcta ggcgaaacac gattttactt gacacaaatt ccatgagata cttcattttc
     2221 tttatattta tggtttctta acatgaataa acttgttagg accacttgct acttgggaat
     2281 tgagtaaaat tgatggtact tgtgtattat ttcccctcat tttttaaaat ataattttaa
     2341 ataaaaacgt gtggagtttt tgtttttagg cttagactag ttgataaatg caagatcctt
     2401 tcaagctttc ccttcttctc tatcactctt ctttcttttt gtcaaaaaat tctctcgcta
     2461 atttatttgc ttttttaatg ttattatttt atgacttttt atagtcactg aaaagtttgc
     2521 atctgagtga agtgaatgct atcaaaatgt gattctgtct gatgtacttt cacgatctct
     2581 cttcaattcc attttgaagt gatttaaacc cgaaaggttg agaaaaatgc gagcgctcaa
     2641 atatttgtat tgtgttcgtt gagtgaccca acaaaaagag gaaactttat tgtgccgcca
     2701 agaaaaaagt cttaattaac ttttcccacc ggtaagagct tgatccggat ctgcggctta
     2761 ctcaccagac ctggccccat ccgattacca cctattcgct tcgatgggac acgcactcgc
     2821 tgagcagcgc ttcgattctt acgaaagtgt gaaaaaatgg ctcgatgaat ggttcgccgc
     2881 aaaagacgat gagttctact ggcgtggaat ccacaaattg cccgagagat gggaaaaatg
     2941 tgtagctagc gacggcaaat actttgaata aatgattttt tctttttcca caaaatttaa
     3001 cgtgtttttt gatttaaaaa aaacgacatt tcatacttgt acacctgata tctagatacc
     3061 taggtgagct ctggtacccc cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     3121 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     3181 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3241 tttcacaccg catatggtgc actctcagtg caatctgctc tgatgccgca tagttaagcc
     3301 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     3361 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     3421 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     3481 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3541 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3601 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3661 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3721 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3781 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     3841 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     3901 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3961 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4021 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4081 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4141 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4201 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4261 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4321 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4381 ggtcagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4441 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4501 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4561 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4621 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4681 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4741 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4801 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4861 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4921 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4981 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5041 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5101 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5161 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5221 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5281 tacggttcct ggtcttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5341 attctgtgga taaccgtatt accgcctttg agtgagctga taccgcaccg tacgtctcga
     5401 ggaattctat caggtgtaca agtatgaaat gtcgtttcga acatatagat gtctcgcaaa
     5461 cgtaaatatt tatcgattgt cataaaactt tgaccttgtg aagtgtcaac cttgactgtc
     5521 gaaccaccat agtttggcgc gaattgagcg tcataattgt ttactctcag tgcagtcaac
     5581 atgtcgagtt tcgtgccgaa taaagagcaa acgcggacag tattaatttt ctgttttcat
     5641 ttgaagaaaa cagctgcggc cggatccttc atcgatgcac ctcttcatcg at
//
