LOCUS       sequence-396315-        8826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible lentiviral expression of Neurod1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Xie G, Lee JE, Senft AD, Park YK, Jang Y, Chakraborty S, Thompson 
            JJ, McKernan K, Liu C, Macfarlan TS, Rocha PP, Peng W, Ge K
  TITLE     MLL3/MLL4 methyltransferase activities control early embryonic 
            development and embryonic stem cell differentiation in a 
            lineage-selective manner.
  JOURNAL   Nat Genet. 2023 Apr 3. doi: 10.1038/s41588-023-01356-4.
  PUBMED    37012455
REFERENCE   2  (bases 1 to 8826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2023 Apr 3. doi: 10.1038/s41588-023-01356-4."
COMMENT     Sequence Label: pCW57.1-Neurod1-3xT7
FEATURES             Location/Qualifiers
     source          1..8826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     421..441
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        423..441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        469..695
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             696..876
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    923..1048
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1541..1774
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1959..2003
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2216..2333
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2387..2701
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2395..2413
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2431..2449
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2466..2484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2502..2520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2538..2556
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2573..2591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2609..2627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2674..2698
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3943..4453
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4372..4390
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4463..5059
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4463..4482)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4959..4979
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5060..5113
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5114..5731,5732..5737,5738..5773,5774..5776,5777..5812,
                     5813..5815,5816..5851,5852..5860)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5900..5920)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6318..6329)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6534..6767
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6839..6960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6876..6895)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6930..6949
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7000..7135
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7135..7206
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7207..7275,7276..8067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7425..7444)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8238..8826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8727..8746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggttttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      421 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      481 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      541 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      601 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      661 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      721 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      781 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      841 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      901 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      961 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1021 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1081 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1141 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1201 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1261 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1321 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1381 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1441 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1501 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1561 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1621 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1681 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1741 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1801 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1861 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1921 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1981 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     2041 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2101 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2161 caaccccgag gggacaattc tcgacctcga gacaaatggc agtattcatc cacaatttta
     2221 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2281 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2341 attacaggga cagcagagat ccactttggc cgcgaatcga tatgtcgagt ttactcccta
     2401 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag
     2461 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     2521 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc
     2581 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg
     2641 taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2701 ctggagaatt ggaattcgcc accatgacca aatcatacag cgagagcggg ctgatgggcg
     2761 agcctcagcc ccaaggtccc ccaagctgga cagatgagtg tctcagttct caggacgagg
     2821 aacacgaggc agacaagaaa gaggacgagc ttgaagccat gaatgcagag gaggactctc
     2881 tgagaaacgg gggagaggag gaggaggaag atgaggatct agaggaagag gaggaagaag
     2941 aagaggagga ggaggatcaa aagcccaaga gacggggtcc caaaaagaaa aagatgacca
     3001 aggcgcgcct agaacgtttt aaattaaggc gcatgaaggc caacgcccgc gagcggaacc
     3061 gcatgcacgg gctgaacgcg gcgctggaca acctgcgcaa ggtggtacct tgctactcca
     3121 agacccagaa actgtctaaa atagagacac tgcgcttggc caagaactac atctgggctc
     3181 tgtcagagat cctgcgctca ggcaaaagcc ctgatctggt ctccttcgta cagacgctct
     3241 gcaaaggttt gtcccagccc actaccaatt tggtcgccgg ctgcctgcag ctcaaccctc
     3301 ggactttctt gcctgagcag aacccggaca tgcccccgca tctgccaacc gccagcgctt
     3361 ccttcccggt gcatccctac tcctaccagt cccctggact gcccagcccg ccctacggca
     3421 ccatggacag ctcccacgtc ttccacgtca agccgccgcc acacgcctac agcgcagctc
     3481 tggagccctt ctttgaaagc cccctaactg actgcaccag cccttccttt gacggacccc
     3541 tcagcccgcc gctcagcatc aatggcaact tctctttcaa acacgaacca tccgccgagt
     3601 ttgaaaaaaa ttatgccttt accatgcact accctgcagc gacgctggca gggccccaaa
     3661 gccacggatc aatcttctct tccggtgccg ctgcccctcg ctgcgagatc cccatagaca
     3721 acattatgtc tttcgatagc cattcgcatc atgagcgagt catgagtgcc cagcttaatg
     3781 ccatctttca cgatggatcc ggtggaagta tggctagcat gactggcggg cagcaaatgg
     3841 gcgggtctag cgcaatggca tctatgaccg gaggtcaaca gatgggaggt tcctcagcca
     3901 tggcgagtat gacaggtggc caacagatgg gttgagttaa ccggggttgg ggttgcgcct
     3961 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4021 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4081 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4141 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4201 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4261 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     4321 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4381 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4441 cctctctccc cagcaattca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4501 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     4561 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     4621 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     4681 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4741 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     4801 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     4861 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     4921 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     4981 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     5041 gacccgcaag cccggtgccg aaggtagagg ttctctcctc acttgtggtg atgttgaaga
     5101 aaaccctggt ccaatgtcta gactggacaa gagcaaagtc ataaacggag ctctggaatt
     5161 actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     5221 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc
     5281 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
     5341 agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg
     5401 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
     5461 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
     5521 cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga
     5581 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
     5641 gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
     5701 cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga
     5761 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
     5821 cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg atcgatccaa
     5881 gatatcgtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5941 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6001 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6061 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6121 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6181 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6241 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6301 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6361 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6421 gggccgcctc cccgcctgtt tcgcctcggc gtccggacta gaggtacctt taagaccaat
     6481 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     6541 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     6601 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6661 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6721 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     6781 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     6841 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6901 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6961 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     7021 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7081 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctttca
     7141 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7201 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7261 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7321 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7381 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7441 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7501 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7561 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7621 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7681 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     7741 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7801 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7861 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7921 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7981 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8041 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8101 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8161 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8221 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8281 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8341 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8401 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8461 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8521 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8581 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8641 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8701 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8761 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8821 tggaaa
//
