LOCUS       sequence-396316-        6221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mesoplasma florum oriC plasmid expressing LanYFP fluorescent 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6221)
  TITLE     Fluorescent protein expression plasmids - Mesoplasma florum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMflT-o4-YFP
FEATURES             Location/Qualifiers
     source          1..6221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3241..3258
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     rep_origin      complement(3341..3929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3421..3440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3935..3953
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     oriT            4011..4120
                     /note="incP origin of transfer"
ORIGIN
        1 tccctttagt gagggttaat gtcgtgactg ggaaaaccct ggcggaattc ggccagtcta
       61 catgtactct ttttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat
      121 tatttaagtg actatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac
      181 tgtcaatttg atagcgggaa caaataatta gatgtccttt tttaggaggg cttagttttt
      241 tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccagag aatatctgta
      301 tgctttgtat acctatggtt atgcataaaa atcccggtga taaaagtatt tatcactggg
      361 atttttatgc ccttttgggt ttttgaatgg aggaaatcta gatgaaaatc ataaatatcg
      421 gtgtattagc tcacgttgat gcaggaaaaa caacattaac tgaatcactt ttatataact
      481 ctggtgcaat tactgaactt ggttcagtag ataaaggtac tactcgtact gataatacat
      541 tattagaacg tcaacgtgga atcacaattc aaacaggtat cacatctttt caatgggaaa
      601 atacaaaagt aaatattata gatacacctg gacacatgga tttccttgca gaagtatacc
      661 gtagtctttc agtattagat ggtgctattt tacttatcag cgctaaagat ggagttcaag
      721 ctcaaactcg tatcttattt cacgcattac gtaaaatggg tattccaaca attttcttta
      781 taaacaaaat tgaccaaaac ggaattgatt taagtacagt ttatcaagat atcaaagaaa
      841 aactttctgc tgaaatcgtt attaaacaaa aagttgaatt atacccaaac gtttgcgtaa
      901 caaattttac tgaatcagaa caatgggata cagttataga aggtaatgat gatttattag
      961 aaaaatacat gtcaggtaaa tcattagaag cattagaatt agaacaagaa gaaagtattc
     1021 gtttccaaaa ctgttcttta ttccctttat accatggaag cgctaaaagt aacataggta
     1081 ttgataactt aattgaagtt attactaaca aattttactc ttcaactcat cgtggtcctt
     1141 ctgaattatg cggtaacgtt ttcaaaattg aatatacaaa aaaacgtcaa cgtttagctt
     1201 atatacgtct ttatagtggt gttttacatt tacgtgatag tgttcgtgtt agtgaaaaag
     1261 aaaagattaa agttacagaa atgtatactt ctattaacgg tgaattatgc aaaattgacc
     1321 gtgcatattc aggtgaaatt gtaattttac aaaacgaatt tcttaaactt aatagtgtac
     1381 ttggtgacac aaaactttta ccacaacgta agaaaattga aaatccacac ccattacttc
     1441 aaacaacagt agaaccaagc aaacctgaac aacgtgaaat gcttttagat gctcttttag
     1501 aaattagtga ctctgaccca cttttacgtt actatgtaga ttctactact catgaaatta
     1561 ttctttcttt ccttggtaaa gttcaaatgg aagttatttc tgcattatta caagaaaaat
     1621 atcatgttga aatcgaatta aaagaaccta ctgtaattta tatggaacgt ccattaaaaa
     1681 atgctgaata tacaattcat attgaagttc caccaaatcc attttgggct tctattggtc
     1741 tttctgtttc tccacttcca cttggtagcg gaatgcaata tgaaagtagc gtaagtttag
     1801 gttatcttaa tcaaagtttc caaaacgcag ttatggaagg tattcgttac ggttgcgaac
     1861 aaggtttata cggttggaat gttacagact gcaaaatctg ttttaagtat ggactttact
     1921 attcacctgt atcaacacct gctgactttc gtatgcttgc accaattgtt ttagaacaag
     1981 ttttaaagaa agctggaact gaacttttag aaccatacct ttcttttaaa atctatgcac
     2041 cacaagaata cttaagtcgt gcttataacg atgcacctaa atactgtgct aatattgttg
     2101 atactcaatt aaagaacaac gaagtaattt taagcggaga aattcctgca cgttgtattc
     2161 aagaatatcg tagtgattta acatttttca ctaatggacg ttctgtttgc ttaactgaat
     2221 taaaaggtta tcatgttact actggtgaac ctgtatgcca accacgtcgt cctaatagtc
     2281 gtattgataa agttcgttat atgttcaaca aaatcacata ataatactag tagcggccgc
     2341 tagagatcaa gccttaacga actaagaccc ccgcaccgaa aggtccgggg gttttttttg
     2401 accttaaaaa cataaccgag gagcagacaa gatcttttcg ccaggcatgc tcataaaaaa
     2461 tttatttgct ttcaggaaat ttttttctgt ataatagatt catacgactc actatactcg
     2521 agaaggaggc aacaagatgt cgttacctgc tacacatgaa ttacatattt tcggtagttt
     2581 taatggtgta gattttgata tggttggaag aggtactggt aatcctaatg atggatatga
     2641 agaattaaat cttaaaagca caaaaggagc tttacaattt tcgccatgga ttttagttcc
     2701 tcagattgga tatggtttcc atcaatatct tccatttcct gatggtatgt caccatttca
     2761 agctgctatg aaagatggat caggttacca agttcataga actatgcaat ttgaagacgg
     2821 agcatctttg actagtaact atagatatac ttacgaagga tcacacatta aaggagaatt
     2881 tcaagttatt ggcacaggtt ttccagctga tggaccagtt atgactaatt cacttactgc
     2941 agcagattgg tgtgttacta aaatgttata tcctaatgat aaaacaataa taagcacttt
     3001 tgattggaca tatactacag gatcaggaaa aagatatcaa agtacagttc gaactaatta
     3061 tacttttgct aaaccaatgg ctgccaatat tttaaaaaat caacctatgt ttgtttttag
     3121 aaaaacagaa ttaaaacatt caaaaactga gctaaatttt aaagaatggc aaaaagcctt
     3181 cactgatgtt atgtaacccg ggcggccgct gcagtaatac gactcaatat agggcatacg
     3241 atttaggtga cactatagta gggataacag ggtaatttag taggtagggc tatatggtcg
     3301 ttatgcgata ctgatgcata aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3721 ggtatgtagg cggtgctaca gagttcttga agttgtggcc taactacggc tacactagaa
     3781 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3901 agattacgcg cagaaaaaaa ggatctcaac gcgcaattaa ccctcactaa agggaacaaa
     3961 agctggagcg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacg ccggccagcc
     4021 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
     4081 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
     4141 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaagcatgg
     4201 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgctg
     4261 gtacccagcg atgaggatga acccactcag ggacctgatg tgacgtgccg tgaacacgag
     4321 cgtgtttagt ttttgatggt tgtcaagttc ttttcattgt attcaaaccc ccttttaaag
     4381 ttgataattc ttgttaatta taatataagt gctggaaaaa gtatagtttt ttattgttat
     4441 ttttaagaaa atttatccat tgtgttttta tatgaacttt ttcaacattt tttataacct
     4501 tttgtggaaa agcggaaaaa agatatataa atactaaact attgcttttt agtgttattc
     4561 attatgttta tttcttttta aaatattttt ttatccacac attgtggaag acttttatta
     4621 ttatgtgttt taatttattt tatgaagggg aattaatatg gaaacaaaag cgctttgaga
     4681 aaaattaatt aataagttaa agaaggaaaa attaatagac caagacatta ttgaagaaca
     4741 tattgttact tcagaattaa ttaaaatttc aaatacagag ttcgtaatcc ttgttagaag
     4801 taatcttggt gtaactattt taaatgaatt taaagaagta tttgtgtacg aatttaaata
     4861 agttttaaac agttatgtgt ctgttgattt tttaactaaa gaaatttttg agaaaaatac
     4921 aaaaaaagag aataaaaaag aacccataaa tactgtttta tctgaaaatg cacttacttt
     4981 tgagaatttt atagttgggt caagtaataa acaagctaat ttagctgcta aaaatgttgt
     5041 tgcaaaccct ggtatgagtt ttaatccttt atttatttat ggagattcag gactgggtaa
     5101 aacacattta ttacaagcta ttaaaaatca agcagaatta aatggaaaaa aagttttata
     5161 tttaacatct gaagaattta caaaaagaat tgttaatgct ttaaacaaag gtgatttaag
     5221 tgaaatagaa gaattaaaaa ctgagataaa ttctaatgaa ttctttattt tagatgatgt
     5281 tcaattttta agtaaaaaag ataaaacaaa cgagtttttc tttaacatta ttaacaattt
     5341 tactgaaaat ggtaaacaat tagttttttc tagtgataag actcctgaat tgttaaatgg
     5401 ttttgataaa agaatgataa ctagatttaa ttcaggttta tcaacaccta ttaatgcttt
     5461 agatattcca actgccaaat tgataattga agctgaaatt aaaaaacaag gtttaaaaca
     5521 aaaaattaaa gaagatgccg ttgtttactt agctcaaaac tttagcgatg atgttagaaa
     5581 aattaaaggt ttagttaata gattactttt ctttggtatt caaaacgatt taggtcacat
     5641 aattgatttg gaagatgtta ttgacttatt taaagataca ccttcagcta atttaggatt
     5701 attaaatgtt aaaaaaatta aagaagttgt tgctaaaaaa tatgatgtta ctataaaagc
     5761 catcgatggt aaagcaagaa caactgctat aaaaaatgct agacatcttt ctatgtattt
     5821 tgcaaaaata attttaaacc atacatcaac tcaaattggt gcagaatttg gtggaagaga
     5881 ccatagtaca gttttaagtg ctatttcgcg tattgaaaaa ttaatatata aagaaaaaga
     5941 attcaaaaaa atagttgagt ctttaaaaaa cgaaataata ggaaaataga tgtaaattcg
     6001 catctatttt ttttgtgtgg aaaagaaatt aaacttgttg aataaaaagt catttaaacc
     6061 tttatttacc gttgttttat gagtgttttc cacatatcaa ctgcattata aatattactt
     6121 atataaagaa taatataata tataaaagca tgcgtatttc ttttgttttt attttataaa
     6181 tatgagataa tatatattgt aaataagagg tgttatatga g
//
