LOCUS       sequence-396318-       10998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLEX_307 plasmid with N-terminal EYFP tag and g418 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10998)
  TITLE     pLEX_307 and pLEX_306 series plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: plex_307-EYFP-ccdB-g418
FEATURES             Location/Qualifiers
     source          1..10998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1288..1297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1294..1296,1297..1299,1300..2010)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1339..1360)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1600..1619)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1947..1968
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    2035..2159
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2184..2189,2190..2207,2208..2214)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2268..2948
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(2334..2353)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3268..3573
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3452..3471
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3614..3738)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3746..3787
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3838..4426
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3891..3911)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4309..4320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4498..4731
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4809..4930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4846..4865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4900..4919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4970..5105
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5125..5144)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5126..5144)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5154..5171)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5154..5170)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5163..5185)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5312..5767
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5399..5418)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5609..5630
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5793..5897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5898..5966,5967..6758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6116..6135)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6929..7517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7418..7437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7671..7688
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7805..7826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7841..7846,7847..7864,7865..7871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7879..7895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7884..7906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7903..7919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7903..7919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7938..7958
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7940..7958
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7986..8212
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8213..8393
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8440..8565
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9058..9291
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9476..9520
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9751..9961
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9773..9969
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9820..9955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9997..10791
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9997..10791
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10051..10070)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10661..10680
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    10839..10956
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag cacccttgcc accatggtga gcaagggcga ggagctgttc
     1321 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1381 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1441 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
     1501 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1561 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1621 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1681 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1741 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1801 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1861 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     1921 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1981 atcactctcg gcatggacga gctgtacaag ggtggaccag gtggtggaca tcaaacaagt
     2041 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
     2101 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
     2161 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
     2221 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     2281 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     2341 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     2401 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     2461 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
     2521 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     2581 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     2641 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     2701 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     2761 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     2821 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     2881 ttaatgaatt acaacagtac tgcgatgagt ggcaggcggg gcgtaatcta gaggatccgg
     2941 cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat
     3001 atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt
     3061 acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct
     3121 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga
     3181 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt
     3241 ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag
     3301 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg
     3361 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtcccccg tgaactttac
     3421 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg
     3481 ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa
     3541 aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag
     3601 tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
     3661 tttatacaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc
     3721 tttcttgtac aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga
     3781 ttctacgtag taatgaacta gtaccggtta agtcgacaat caacgcgtta agtcgacaat
     3841 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3901 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3961 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4021 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4081 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4141 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4201 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     4261 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4321 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4381 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     4441 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4501 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4561 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4621 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4681 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     4741 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4801 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4861 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4921 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4981 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5041 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5101 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     5161 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5221 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5281 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     5341 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5401 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5461 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5521 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5581 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5641 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5701 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5761 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5821 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5881 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5941 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6001 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6061 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6121 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6181 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6241 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6301 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6361 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6421 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6481 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6541 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6601 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6661 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6721 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6781 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6841 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6901 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6961 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7021 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7081 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7141 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7201 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7261 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7321 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7381 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7441 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7501 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7561 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7621 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7681 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7921 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7981 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     8041 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     8101 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     8161 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     8221 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8281 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8341 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     8401 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     8461 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     8521 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     8581 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     8641 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     8701 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     8761 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     8821 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     8881 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     8941 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     9001 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     9061 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     9121 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     9181 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     9241 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     9301 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     9361 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     9421 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     9481 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     9541 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     9601 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     9661 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     9721 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     9781 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9841 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9901 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9961 ttttgcaaaa agctttctag aggtaccgtg gccaccatga ttgaacaaga tggattgcac
    10021 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
    10081 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
    10141 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
    10201 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
    10261 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
    10321 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
    10381 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
    10441 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
    10501 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
    10561 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
    10621 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
    10681 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
    10741 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agttaacgaa
    10801 ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
    10861 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
    10921 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
    10981 gatccacttt ggcgccgg
//
