LOCUS       sequence-396319-        9516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible lentiviral expression of Gata6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Xie G, Lee JE, Senft AD, Park YK, Jang Y, Chakraborty S, Thompson 
            JJ, McKernan K, Liu C, Macfarlan TS, Rocha PP, Peng W, Ge K
  TITLE     MLL3/MLL4 methyltransferase activities control early embryonic 
            development and embryonic stem cell differentiation in a 
            lineage-selective manner.
  JOURNAL   Nat Genet. 2023 Apr 3. doi: 10.1038/s41588-023-01356-4.
  PUBMED    37012455
REFERENCE   2  (bases 1 to 9516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2023 Apr 3. doi: 10.1038/s41588-023-01356-4."
COMMENT     Sequence Label: pCW57.1-Gata6-3xT7
FEATURES             Location/Qualifiers
     source          1..9516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..234
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    306..427
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(343..362)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     397..416
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      467..602
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        602..673
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(674..742,743..1534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(892..911)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1705..2293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2194..2213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2447..2464
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2581..2602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2617..2622,2623..2640,2641..2647)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2655..2671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2660..2682
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2679..2695
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2679..2695
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2714..2734
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2716..2734
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2762..2988
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2989..3169
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3216..3341
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3834..4067
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4252..4296
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4509..4626
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4680..4994
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4688..4706
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4724..4742
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4759..4777
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4795..4813
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4831..4849
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4866..4884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4902..4920
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4967..4991
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5005..5014
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5974..5994
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     promoter        6926..7436
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7355..7373
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7446..8042
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7446..7465)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7942..7962
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             8043..8096
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(8097..8714,8715..8720,8721..8756,8757..8759,8760..8795,
                     8796..8798,8799..8834,8835..8843)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(8883..8903)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9301..9312)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
       61 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      121 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      181 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
      241 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
      301 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      361 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      421 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
      481 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
      541 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
      601 cctttcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      661 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      721 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      781 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      841 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      901 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      961 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1021 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     1081 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1141 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     1201 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     1261 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     1321 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     1381 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     1441 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     1501 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     1561 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1621 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1681 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1741 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1801 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1861 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1921 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1981 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2041 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2101 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2161 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2221 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2281 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2341 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2401 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     2461 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     2521 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     2581 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2641 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2701 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     2761 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     2821 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     2881 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     2941 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     3001 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     3061 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     3121 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     3181 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     3241 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     3301 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     3361 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     3421 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     3481 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     3541 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     3601 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     3661 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     3721 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     3781 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     3841 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     3901 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     3961 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     4021 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     4081 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     4141 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     4201 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     4261 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     4321 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     4381 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     4441 cagacccacc tcccaacccc gaggggacaa ttctcgacct cgagacaaat ggcagtattc
     4501 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     4561 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     4621 aattttcggg tttattacag ggacagcaga gatccacttt ggccgcgaat cgatatgtcg
     4681 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
     4741 gaacgatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     4801 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     4861 gtttatccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     4921 acgtatgtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga
     4981 accgtcagat cgcctggaga attggccacc atggccttga ctgacggcgg ctggtgcctg
     5041 ccgaagcgtt tcggggctgc tgctgcggac gccggcgact ccgggccctt tccagcgcgg
     5101 gagccctcct cgccgctttc ccccatctcg tcttcgtcct cctcctgctc ccggggcggg
     5161 gatcgcggtc cctgcggcgc cagcaactgc aggacgccgc agctcgacgc cgaggcggtg
     5221 gcgggacctc cgggccgctc gctcttgctc agcccctacg cctcgcatcc cttcgccgct
     5281 gcccacggag ccgcggcgcc cggggtcgca ggccccggga gcgccctgtc gacttgggag
     5341 gacctgttgc tcttcactga cctcgatcag gccgcgaccg ccagcaagct gttgtggtcc
     5401 agccggggcg ccaaactgag ccccttcgcg gccgagcagc cggaggaaat gtaccagacc
     5461 ctcgccgccc tgtccagcca ggggcccgcc gcttacgacg gcgcgcccgg cggcttcgtg
     5521 cactccgcag cggcggcggc cgctgccgcc gcggcagcca gctcccctgt ctacgtgccc
     5581 accacgcgcg tgggctccat gctgtccggc ctgccctacc ttcaaggcgc gggcagcggg
     5641 cccagcaatc acgcgggcgg agcgggtgcc cacccaggct ggtcccaggc ctccgccgac
     5701 agccccccgt atggcggggg tggcgcagcc ggcggcggcg cggccggacc tggaggtgct
     5761 ggatcggcta cggcccacgc ctctgcacgg tttccctact cgcccagccc gcccatggcc
     5821 aacggcgccg cgcgagaccc cgggggctac gtggctgcgg gcggcacggg cgcaggcagt
     5881 gtgagtggag gtggcggcag cctggcggcc atgggtggcc gggagcacca gtacagctcg
     5941 ctgtccgcag ctcggccgct gaacggaacg taccaccacc accatcacca tcacccgacc
     6001 tactcgccct acatggccgc accgctgact cctgcctggc cagcaggacc cttcgaaacg
     6061 ccggtgctcc acagcttaca gggccgcgcg ggagctccac tcccggtgcc acgaggcccc
     6121 agcacagacc tgttggagga cctgtcggag agccgcgagt gcgtgaactg cggctccatc
     6181 cagacgccac tgtggagacg agacggcacc ggtcattacc tgtgcaatgc atgcggtctc
     6241 tacagcaaga tgaatggcct cagcaggccc ctcatcaagc cacagaagcg cgtgccttca
     6301 tcacggcggc ttggactgtc ctgtgccaac tgtcacacca caaccactac cttatggcgt
     6361 agaaatgctg agggtgagcc tgtgtgcaat gcttgcgggc tctatatgaa actccatggg
     6421 gtgcctcgac cacttgctat gaaaaaagaa ggaattcaaa ccaggaaacg aaaacctaaa
     6481 aatataaata agtcaaaagc ttgctccggt aacagcagtg gctctgtccc tatgactcct
     6541 acttcctctt cttctaattc agatgactgc accaaaaata cttctccttc tacacaagcg
     6601 accacctcag gggtaggggc atcagtgatg tctgcagtgg gagaaaacgc caaccccgag
     6661 aacagtgacc tcaagtattc aggtcaagac ggcctctaca taggtgtcag tctgtcctcc
     6721 cctgccgaag tcacatcctc cgtgcgacag gattcttggt gtgctctggc cctggccgga
     6781 tccggtggaa gtatggctag catgactggc gggcagcaaa tgggcgggtc tagcgcaatg
     6841 gcatctatga ccggaggtca acagatggga ggttcctcag ccatggcgag tatgacaggt
     6901 ggccaacaga tgggttgagt taaccggggt tggggttgcg ccttttccaa ggcagccctg
     6961 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     7021 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     7081 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     7141 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     7201 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     7261 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     7321 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     7381 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccagcaat
     7441 tcaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     7501 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     7561 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     7621 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     7681 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     7741 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     7801 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     7861 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     7921 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     7981 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     8041 ccgaaggtag aggttctctc ctcacttgtg gtgatgttga agaaaaccct ggtccaatgt
     8101 ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta
     8161 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     8221 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     8281 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     8341 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     8401 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     8461 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     8521 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     8581 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
     8641 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     8701 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     8761 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     8821 accttgacat gctccccggg taactaagta aggatcgatc caagatatcg tattcttaac
     8881 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     8941 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     9001 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     9061 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     9121 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     9181 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     9241 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     9301 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     9361 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     9421 gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc aatgacttac aaggcagctg
     9481 tagatcttag ccacttttta aaagaaaagg ggggac
//
