LOCUS       sequence-396320-        9519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes recombinant mouse VEGF120 when coupled with amber stop codon
            suppression by non-canonical amino acid incorporation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Miller LN, Walters AE, Denninger JK, Hanson MA, Marshall AH, 
            Johantges AC, Hosawi M, Sebring G, Rieskamp JD, Ding T, Rindani R, 
            Chen KS, Goldberg ME, Senthilvelan S, Volk A, Zhao F, Askwith C, 
            Wester JC, Kirby ED
  TITLE     Neural stem and progenitor cells support and protect adult 
            hippocampal function via vascular endothelial growth factor 
            secretion.
  JOURNAL   Mol Psychiatry. 2024 Nov 11. doi: 10.1038/s41380-024-02827-8.
  PUBMED    39528687
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Psychiatry. 2024 Nov 11. doi: 10.1038/s41380-024-02827-8."
COMMENT     Sequence Label: VEGF120TAG
FEATURES             Location/Qualifiers
     source          1..9519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     87..107
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        266..1444
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          497..1435
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1392..1412
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1461..1479
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1940..1957
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    1980..2568
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2033..2053)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2451..2462)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2601..2825
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    2867..2984
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3033..3543
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3462..3480
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3565..4164
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3565..3584)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4061..4081
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4292..4525
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4597..4718
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4634..4653)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4688..4707
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4758..4893
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4914..4932)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4942..4959)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4942..4958)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4951..4973)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5100..5555
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5187..5206)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5397..5418
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5581..5685
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5686..5754,5755..6546)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5904..5923)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6717..7305
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7206..7225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7459..7476
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7593..7614
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7629..7634,7635..7652,7653..7659)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7667..7683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7672..7694
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7691..7707
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7691..7707
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7726..7746
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7728..7746
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7774..8000
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8001..8181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8228..8353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8846..9079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9264..9308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact
       61 agcctcgagc ggccgccccc ttcaccgagg gcctatttcc catgattcct tcatatttgc
      121 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
      181 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
      241 aattatgttt taaaatggac tatcaggctc cggtgcccgt cagtgggcag agcgcacatc
      301 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
      361 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
      421 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
      481 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
      541 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
      601 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
      661 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
      721 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
      781 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
      841 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
      901 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
      961 agctggccgg cctgctctgg tgcctggtct cgcgccgccg tgtatcgccc cgccctgggc
     1021 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     1081 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     1141 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     1201 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     1261 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     1321 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     1381 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     1441 gtgaggaatt agcttggtac taatacgact cactataggg agacccaagc tggctagcat
     1501 gaactttctg ctctcttggg tgcactggac cctggcttta ctgctgtacc tccaccatgc
     1561 caagtggtcc caggctgcac ccacgacaga aggagagtag cagaagtccc atgaagtgat
     1621 caagttcatg gatgtctacc agcgaagcta ctgccgtccg attgagaccc tggtggacat
     1681 cttccaggag taccccgacg agatagagta catcttcaag ccgtcctgtg tgccgctgat
     1741 gcgctgtgca ggctgctgta acgatgaagc cctggagtgc gtgcccacgt cagagagcaa
     1801 catcaccatg cagatcatgc ggatcaaacc tcaccaaagc cagcacatag gagagatgag
     1861 cttcctacag cacagcagat gtgaatgcag accaaagaaa gacagaacaa agccagaaaa
     1921 atgtgacaag ccaaggcggc atcatcatca tcatcattaa cgatatcaag cttatcgata
     1981 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2041 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2101 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2161 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2221 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2281 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2341 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     2401 cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     2461 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     2521 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag
     2581 cgctgctcga gagatctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     2641 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     2701 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     2761 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     2821 tatgggaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2881 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2941 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     3001 acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc
     3061 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     3121 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     3181 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     3241 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     3301 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     3361 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     3421 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     3481 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     3541 cagggggatc caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     3601 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     3661 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     3721 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     3781 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     3841 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     3901 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     3961 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     4021 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     4081 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     4141 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
     4201 agcgcacgac cccatgcatc ggtaccttta agaccaatga cttacaaggc agctgtagat
     4261 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     4321 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     4381 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4441 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4501 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     4561 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     4621 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4681 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     4741 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4801 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     4861 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     4921 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     4981 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     5041 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     5101 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5161 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5221 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5281 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     5341 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5401 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     5461 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5521 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     5581 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5641 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5701 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5761 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5821 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5881 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5941 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6001 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6061 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6121 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6181 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6241 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6301 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6361 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6421 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6481 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6541 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6601 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6661 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6721 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6781 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6841 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6901 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6961 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7021 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7081 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7141 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7201 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7261 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7321 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7381 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7441 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7501 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7561 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7621 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7681 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     7741 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     7801 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     7861 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     7921 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     7981 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     8041 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8101 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8161 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     8221 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     8281 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     8341 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     8401 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     8461 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     8521 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     8581 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     8641 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     8701 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     8761 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     8821 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     8881 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     8941 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     9001 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     9061 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     9121 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     9181 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     9241 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     9301 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     9361 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     9421 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     9481 ccgacaggcc cgaaggaata gaagaagaag gtggagaga
//
