LOCUS       sequence-396322-        9954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLEX_307 plasmid with N-terminal FLAG tag and blasticidin marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9954)
  TITLE     pLEX_307 and pLEX_306 series plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: plex_307-3xflag-ccdb-blasticidin
FEATURES             Location/Qualifiers
     source          1..9954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1288..1297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1297..1362
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    1387..1511
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1536..1541,1542..1559,1560..1566)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1620..2300
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENVFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(1686..1705)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2620..2925
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2804..2823
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2966..3090)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3098..3139
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3190..3778
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3243..3263)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3661..3672)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3850..4083
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4161..4282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4198..4217)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4252..4271
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4322..4457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4477..4496)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4478..4496)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4506..4523)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4506..4522)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4515..4537)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4664..5119
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4751..4770)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4961..4982
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5145..5249
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5250..5318,5319..6110)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5468..5487)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6281..6869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6770..6789
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7023..7040
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7157..7178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7193..7198,7199..7216,7217..7223)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7231..7247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7236..7258
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7255..7271
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7255..7271
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7290..7310
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7292..7310
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7338..7564
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7565..7745
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7792..7917
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8410..8643
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8828..8872
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9103..9313
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9125..9321
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9172..9307
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      9343..9352
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9349..9747
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9795..9912
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag cacccttgcc accatggact acaaagacca tgacggtgat
     1321 tataaagatc atgacatcga ttacaaggat gacgatgaca agggtggacc aggtggtgga
     1381 catcaaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     1441 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     1501 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     1561 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     1621 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     1681 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     1741 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     1801 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     1861 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     1921 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     1981 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     2041 atgtgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     2101 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     2161 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     2221 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcaggcg gggcgtaatc
     2281 tagaggatcc ggcttactaa gagccagata acagtacgcg tatttgcgcg ctgatttttg
     2341 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg
     2401 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
     2461 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
     2521 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
     2581 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc
     2641 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
     2701 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtcccc
     2761 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     2821 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     2881 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt
     2941 atacacagcc agtctgcagg tcgaccatag tgactggata cgttgtgttt tacagtatta
     3001 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
     3061 ttctcgttca gctttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct
     3121 cctcggtctc gattctacgt agtaatgaac tagtaccggt taagtcgaca atcaacgcgt
     3181 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3241 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3301 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3361 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3421 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3481 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3541 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3601 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3661 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3721 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     3781 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3841 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     3901 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3961 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4021 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4081 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     4141 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     4201 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4261 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     4321 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4381 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4441 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     4501 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4561 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4621 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     4681 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4741 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4801 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4861 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4921 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4981 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5041 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5101 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5161 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5221 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5281 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5341 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5401 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5461 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5521 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5581 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5641 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5701 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5761 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5821 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5881 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5941 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6001 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6061 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6121 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6181 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6241 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6301 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6361 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6421 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6481 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6541 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6601 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6661 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6721 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6781 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6841 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6901 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6961 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7021 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7081 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7141 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7201 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7261 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     7321 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     7381 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     7441 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     7501 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     7561 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7621 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7681 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7741 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     7801 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     7861 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     7921 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     7981 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     8041 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     8101 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     8161 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     8221 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     8281 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     8341 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     8401 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     8461 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     8521 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     8581 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     8641 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     8701 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     8761 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     8821 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     8881 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     8941 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     9001 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
     9061 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9121 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     9181 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9241 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     9301 ggaggcctag gcttttgcaa aaagctttct agaggtaccg tggccaccat ggccaagcct
     9361 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     9421 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     9481 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     9541 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     9601 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     9661 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     9721 ccctctggtt atgtgtggga gggctaagtt aacgaattct agatcttgag acaaatggca
     9781 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     9841 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     9901 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg ccgg
//
