LOCUS       sequence-396323-        7815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For bacterial expression of the APOBEC1-YTH fusion protein for 
            protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Zhu H, Yin X, Holley CL, Meyer KD
  TITLE     Improved Methods for Deamination-Based m(6)A Detection.
  JOURNAL   Front Cell Dev Biol. 2022 Apr 27;10:888279. doi: 
            10.3389/fcell.2022.888279. eCollection 2022.
  PUBMED    35573664
REFERENCE   2  (bases 1 to 7815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Dev Biol."; date: "2022-04-27"; volume: "10:888279. doi"; pages: " 
            10.3389/fcell.2022.888279. eCollection 2022"
COMMENT     Sequence Label: pET-APOBEC1-YTH
FEATURES             Location/Qualifiers
     source          1..7815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     120..139
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     223..242
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        223..241
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    242..266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             281..303
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             322..339
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             349..1449
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1423..1446
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1462..1482
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1486..2172
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             2809..2835
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2901..2918
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2971..2989)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2985..3032
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3069..3524
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3156..3175)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3366..3387
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3617..4432)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3617..4432)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4340..4359
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4424..4443
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4554..5142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5043..5062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5296..5313
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5328..5467
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5393..5412)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5553..5575
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5569..5760)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6569..7651)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7613..7632
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7652..7729)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
       61 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
      121 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
      181 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
      241 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
      301 agatatacca tgggttcttc tcaccatcac catcaccatg gttcttctat gaaaatcgaa
      361 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
      421 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
      481 gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca
      541 cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
      601 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
      661 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
      721 aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
      781 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
      841 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg
      901 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac
      961 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     1021 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     1081 gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc
     1141 gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat
     1201 ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg
     1261 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac tatggaaaac
     1321 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     1381 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     1441 gcgcagacta atgggatcga ggaaaacctg tacttccaat ccaatatgag cagcgaaacc
     1501 ggtccggtgg cggttgaccc gaccctgcgt cgtcgtattg agccgcacga gttcgaagtg
     1561 ttctttgatc cgcgtgagct gcgtaaggaa acctgcctgc tgtacgaaat taactggggt
     1621 ggccgtcaca gcatctggcg tcacaccagc cagaacacca acaagcacgt tgaggtgaac
     1681 ttcatcgaaa aatttaccac cgagcgttac ttctgcccga acacccgttg cagcattacc
     1741 tggtttctga gctggagccc gtgcggtgaa tgcagccgtg cgatcaccga gttcctgagc
     1801 cgttatccgc acgttaccct gtttatctac attgcgcgtc tgtatcacca cgcggacccg
     1861 cgtaaccgtc aaggtctgcg tgatctgatc agcagcggcg tgaccatcca gattatgacc
     1921 gagcaagaaa gcggttactg ctggcgtaac ttcgttaact atagcccgag caacgaagcg
     1981 cattggccgc gttacccgca cctgtgggtg cgtctgtacg ttctggagct gtattgcatc
     2041 attctgggcc tgccgccgtg cctgaacatt ctgcgtcgta agcagccgca actgaccttc
     2101 tttaccatcg cgctgcagag ctgccactac caacgtctgc cgccgcacat tctgtgggcg
     2161 accggtctga agagcggcag cgaaaccccg ggtaccagcg aaagcgcgac cccggagccg
     2221 cacccggtgc tggagaaact gcgtagcatc aacaactata acccgaagga cttcgattgg
     2281 aacctgaaac acggtcgtgt ttttatcatt aagagctaca gcgaagacga tatccaccgt
     2341 agcattaaat ataacatctg gtgcagcacc gagcacggca acaagcgtct ggacgcggcg
     2401 taccgtagca tgaacggtaa aggcccggtg tatctgctgt tcagcgttaa cggtagcggc
     2461 cacttttgcg gtgtggcgga aatgaaaagc gcggttgatt acaacacctg cgcgggtgtg
     2521 tggagccagg acaagtggaa aggccgtttc gatgttcgtt ggatttttgt gaaggacgtt
     2581 ccgaacagcc aactgcgtca catccgtctg gagaacaacg aaaacaaacc ggtgaccaac
     2641 agccgtgata cccaggaagt gccgctggaa aaggcgaaac aagttctgaa gatcattgcg
     2701 agctacaaac acaccaccag catcttcgac gattttagcc actatgagaa gcgtcaggaa
     2761 gaggaagaga gcgtgaagaa ggagcgtcaa ggtcgtggca aactggagta cccgtatgac
     2821 gttccggatt atgcgtaaat tggaagtgga taacggatcc gaattcgagc gccgtcgaca
     2881 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
     2941 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
     3001 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
     3061 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3121 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3181 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3241 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3301 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3361 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     3421 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     3481 aaaatttaac gcgaatttta acaaactagt aacgtttaca atttcaggtg gcacttttcg
     3541 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3601 gctcatgaat taattcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     3661 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     3721 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     3781 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     3841 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
     3901 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     3961 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     4021 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatgtttt
     4081 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
     4141 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     4201 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     4261 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     4321 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     4381 tggaatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc ataacacccc
     4441 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgaccaaaat cccttaacgt
     4501 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4561 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4621 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4681 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     4741 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4801 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4861 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4921 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4981 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5041 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5101 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5161 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5221 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     5281 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     5341 tttctcctta cgcatctgtg cggtatttca caccgcaatg gtgcactctc agtacaatct
     5401 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     5461 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     5521 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     5581 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag
     5641 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     5701 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     5761 tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga
     5821 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga
     5881 gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg
     5941 ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat
     6001 gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac
     6061 acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc
     6121 gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca
     6181 gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtgggg ccgccatgcc
     6241 ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg
     6301 agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca
     6361 gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg
     6421 catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa
     6481 ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt
     6541 gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6601 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6661 ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct
     6721 ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct
     6781 gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca
     6841 ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca
     6901 gcgccatctg atcgttggca accagcatcg cagtgggaac gataccctca ttcagcattt
     6961 gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct
     7021 gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag
     7081 aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca
     7141 cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag
     7201 agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct
     7261 ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca
     7321 ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac
     7381 ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca
     7441 gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc
     7501 ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag
     7561 aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact
     7621 ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg
     7681 ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga
     7741 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg
     7801 agcaccgccg ccgca
//
