LOCUS       sequence-396327-       10915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression in mammalian cells or oocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Abderemane-Ali F, Rossen ND, Kobiela ME, Craig RA, Garrison CE, Chen
            Z, Colleran CM, O'Connell LA, Du Bois J, Dumbacher JP, Minor DL
  TITLE     Evidence that toxin resistance in poison birds and frogs is not 
            rooted in sodium channel mutations and may rely on ""toxin sponge"" 
            proteins.
  JOURNAL   J Gen Physiol. 2021 Sep 6;153(9):e202112872. doi: 
            10.1085/jgp.202112872. Epub 2021 Aug 5.
  PUBMED    34351379
REFERENCE   2  (bases 1 to 10915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Physiol."; date: "2021-09-6"; volume: "153(9):e202112872. doi"; 
            pages: " 10.1085/jgp.202112872. Epub 2021 Aug 5"
COMMENT     Sequence Label: Pum NaV1.4-N830T
FEATURES             Location/Qualifiers
     source          1..10915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6506..6523)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6512..6736
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6782..7210
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6869..6888)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7079..7100
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7219..7239)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7224..7553
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7404..7539
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7620..8414
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7674..7693)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8284..8303
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8588..8709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8625..8644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8679..8698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8758..8774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8758..8774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8771..8793)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8782..8798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8806..8812,8813..8830,8831..8836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8851..8872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8989..9006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9160..9748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9240..9259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9919..10710,10711..10779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10542..10561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10780..10884)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagatggctg ggctgcctca tatccccgga cccgagaacc tgagacactt
      961 cacacccgct tcactggctg ctatcgaaca gagaatggcc gaggccgagg ccctgcggat
     1021 caagagacag caggtggaga tcccagagga ggaggaggtg aagcccagct ccgatctgga
     1081 ggccggcaag aacctgcctc tgatctacgg cgacccacct ctggagctga tcggaacccc
     1141 tctggaggat ctggacccat tctataagga caagaagacc ttcatcgtgc tgaacaaggg
     1201 caagagcatc tttaggttct ccgccacccc agccctgtac atgctgggac ctttccaccc
     1261 aatccggaga ggcgccatca aggtgctggt gcactccctg ttttctatgt tcatcatgat
     1321 caccatcctg acaaactgcg tgtttatgac catgtctaac ccacccgcct ggagcaagaa
     1381 tgtggagtac gccttcaccg gcatctatac atttgagtcc ctgatcaaga tcctgtctcg
     1441 gggcttctgt atcgacgact tcaccttcct gcgggatcca tggaactggc tggacttcat
     1501 ggtcatcagc atggcctaca tcaccgagtt tgtggacctg ggcaatatct ccgccctgag
     1561 gacatttcgc gtgctgcggg ccctgaagac catcacagtg atcccaggcc tgaagaccat
     1621 cgtgggcgcc ctgatccagt ctgtgaagaa gctgagcgac gtgatgatcc tgacagtgtt
     1681 ctgcctgagc gtgtttgccc tgatcggcct gcagctgttc atgggcaacc tgaggcagaa
     1741 gtgcgtgcgg tggcctccac ccaatggcac ctttctggag gatctgggcc tggataacga
     1801 cacactggtg aattccacct tttacgactt catggacgac ttcaccggca acttcacagg
     1861 caacttcacc ggaaacttca catctaatag cacctttgat ttcgaggcct atatcaacga
     1921 cgaggccaat tactatttcc tggagggcgc cctggatgca ctgctgtgcg gcaactctag
     1981 cgacgccggc aagtgcccag agggatacga gtgtatgaag gccggccgga accccaatta
     2041 tggctacaca tcctatgata ccttttcttg ggccttcctg gccctgttta ggctgatgac
     2101 ccaggactac tgggagaatc tgttccagct gacactgcgc gcagcaggca agacctatat
     2161 gatcttcttt gtggtggtca tcttcctggg cagcttttac ctgatcaacc tgatcctggc
     2221 cgtggtggcc atggcctatg ccgagcagaa tgatgccacc atgctggagg agcagcagaa
     2281 ggaggaggag ttccagcagc tgctggagca gctgaagaag cagcaggagg agcaggagaa
     2341 gctgctgcag gagagaacat cctctagcga cagcctgcca tcctctgtgg caggcaaggg
     2401 acacgatagc gacctgaact ccgatcagga caaggccaag gattgtaatg gccaggtggt
     2461 gccaaagatc acactgcaga ggtcccacac cgtgatcgac ttcaacccca cccacgagcc
     2521 cgagaagcct gatctgaacc acctgacagt gggcaatcag gacggacctg gactggagaa
     2581 gagggtggga agcgccatct ccgtgatctc taacaccgtg gaggagctgg aggaggccca
     2641 ccagaagtgc cctccatggt ggtacaagtt cgcccacaca tttctggtgt ggaattgctg
     2701 tcctctgtgg gtgaagctga aggagttcgt gaagctggtg gtcatggatc catttgtgga
     2761 cctgggcatc acaatctgta tcgtgctgaa caccctgttc atggccatgg agcactatcc
     2821 tatgaccgag gagtttgaga acgtgctgag cgtgggcaat ctggtgttca caggcatctt
     2881 taccgccgag atggtgctga agctgatcgc cctggatcca tatgagtact tccagcaggg
     2941 ctggaacatc tttgacagca tcatcgtgac cctgtccctg gtggagctgg gactggccaa
     3001 tgtgcagggc ctgtctgtgc tgagaagctt caggctgctg cgcgtgttta agctggccaa
     3061 gtcttggccc accctgaaca tgctgatcaa gatcatcggc aacagcgtgg gcgccctggg
     3121 caatctgaca ctggtgctgg ccatcatcgt gtttatcttc gccgtggtgg gcatgcagct
     3181 gttcggcaag agctacatcg agtgcgtgtg caagatctcc gtggattgca ccctgcctcg
     3241 gtggcacatg aatgacttct ttcacagctt cctgatcgtg tttagaatcc tgtgcggcga
     3301 gtggatcgag accatgtggg attgcatgga ggtggccggc cagacaatgt gcctgatcgt
     3361 gttcatgatg gtcatggtca tcggcaacct ggtggtgctg actctgttcc tggccctgct
     3421 gctgagctcc tttagcgccg actccctggc agcatccgac gatgacggcg agatgaacaa
     3481 tctgcagatc gccatcggcc ggatcaccag aggcatcgcc ttcgccaagg ccacactgct
     3541 ggccctgctg aggcgcctgt ggaagggcaa gaaggtggcc cccgaggagg agcaggatcc
     3601 tagcaagagg gactctagcg tgctgaacca cgtggatacc gcccctgagc caaagtccgg
     3661 ctacctggac ggcatcacag gcaaggagca catcttcatg gatgagctgg accacatgaa
     3721 ctttatcaac aatcctaatc tgaccgtgca ggtgccaatc gcctctgagg agagcgacct
     3781 gtatgaggag acctccacac agtctgagac cgaggatacc acaaagaacc ctctgtcctc
     3841 tgacaatgcc tccgccgtgt gctctacagt ggatgacaag ccacctgcac ctgaagagga
     3901 ggaggtggca gaggtggcag aggagtccaa cgagcctgag gagtgcttca ccgagggctg
     3961 cgtgaagcgg ttcccatgcc tgtacgtgga catcacatct gagaagggca agatctggtg
     4021 gaatctgcgg aagacctgtt tcagaatcgt ggagcacgac tggtttgaga cattcatcgt
     4081 gtttatgatc ctgctgagct ccggcgccct ggcctttgag gacatctaca tcgagcagcg
     4141 gaaggtggtg agaaccatcc tggagtacgc cgacaaggtg ttcagctata tctttgtgat
     4201 cgagatgctg ctgaagtggg tggcctacgg cttcaaggtg tacttcacca acgcctggtg
     4261 ctggctggat ttcctgatcg tggacgtgag cctgatctcc ctgaccgcca attggctggg
     4321 ctactctgag ctgggcgcca tcaagagcct gaggacactg cgcgccctgc ggcccctgag
     4381 agccctgagc aggttcgagg gcatgagggt ggtggtgaac gcactgctgg gagcaatccc
     4441 ttccatcatg aatgtgctgc tggtgtgcct gatcttctgg ctgatctttt ctatcatggg
     4501 cgtgaacctg ttcgccggca agtactatcg gtgcgtgaat accacaaccg gcgagctgtt
     4561 tgagatcgat gtggtgaaca ataagtccga ctgtatggcc ctgctgcaca ccaacgaggt
     4621 gagatgggtg aatgtgaagg tgaacttcga caatgtgggc ctgggctacc tgtctctgct
     4681 gcaggtggcc accttcaagg gctggatgga catcatgtac gccgccgtgg acagcaggga
     4741 gatcgagcag cagccccagt atgagatcaa cctgtacatg tacatctatt tcgtgatctt
     4801 tatcatcttc ggcgccttct ttaccctgaa tctgttcatc ggcgtgatca tcgataactt
     4861 taatcagcag aagaagaagt tcggcggcaa ggacatcttt atgacagagg agcagaagaa
     4921 gtactataac gccatgaaga agctgggctc caagaagccc cagaagccca tccctcgccc
     4981 atctaataag ttccagggca aggtgtttga tttcgtgaca aagcaggtgt ttgacatcac
     5041 catcatgatc ctgatctgcc tgaacatggt gacaatgatg gtggagaccg atgaccagag
     5101 cgagctgaag acctccgtgc tgtacaagat caatctggtg ttcatcgtga tctttacagg
     5161 cgagtgcgtg ctgaagatgt tcgccctgcg gtactattac tttaccgtcg gatggaacat
     5221 ctttgatttc gtggtggtca tcctgagcat cctgggcatc gtgctgtctg acatcatcga
     5281 gaagtatttc gtgagcccca ccctgttcag agtgatcagg ctggcaagga tcggaagagt
     5341 gctgaggctg atcaggggag caaagggaat cagaacactg ctgttcgccc tgatgatgtc
     5401 tctgcctgcc ctgtttaaca tcggcctgct gctgttcctg gtcatgttta tctactctat
     5461 cttcggcatg agcaattttg gctatgtgaa gaaggagtcc ggcatcgatg acatctttaa
     5521 cttcgagacc ttcggcaatt ctatgatctg cctgtttcag atcacaacca gcgccggatg
     5581 ggatggactg ctgaacccaa tcctgaatag cggcccaccc gattgtgacc cagagctgga
     5641 gaacccagga tctagcgtga agggcgactg cggcaatccc tccatcggca tcttcttttt
     5701 ctgttcctac atcatcatct ctttcctgat cgtggtgaac atgtatatcg ccatcatcct
     5761 ggagaacttc agcgtggcca ccgaggagtc ctctgagcca ctgtgcgagg atgactttga
     5821 gatgttctac gagacatggg agaagttcga tcccgacgcc acacagttta tcgcctattc
     5881 taccctgagc gatttcgtgg acacactgca ggagcccctg aagatcccca agcctaacaa
     5941 gatcaagctg atcaccatgg acctgcctat ggtggccggc gataagatcc actgcctgga
     6001 catcctgttc gccctgacca aggaggtgct gggcgatagc ggcgagatgg acgccctgaa
     6061 ggccacaatg gaggagaagt ttatggccgc caatccctcc aaggtgtctt acgagcctat
     6121 cacaaccaca ctgaagagga agcacgagga ggtgtgcgca accaagatcc agagggcctt
     6181 ccggcggtac ctgctgcggc ggagcgtgaa gcaggcatcc tacatgtata gacactccca
     6241 ggataccctg ggagaggacg caccagagaa ggagggactg atcgcaacaa agatgcagga
     6301 gatctacggc aacaccggca cagatgtgga gaccgcaccc gccctgggac tggagccaat
     6361 ccccagctcc gagacacctc aggacgcccc cgaggaggct caggcttgga ataaggagag
     6421 ggtggtgaaa gagagtctgg tgtaggatat ccagcacagt ggcggccgct cgagtctaga
     6481 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     6541 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6601 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     6661 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     6721 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     6781 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6841 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6901 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6961 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7021 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7081 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7141 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7201 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     7261 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     7321 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7381 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     7441 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     7501 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     7561 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     7621 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     7681 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     7741 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     7801 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     7861 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     7921 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7981 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     8041 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     8101 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     8161 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     8221 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     8281 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     8341 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     8401 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     8461 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     8521 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     8581 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8641 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8701 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8761 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8821 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8881 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8941 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9001 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9061 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9541 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9601 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9841 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9901 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9961 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    10021 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    10081 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    10141 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    10201 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10261 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10321 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10381 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10441 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10501 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10561 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10621 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10681 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10741 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10861 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
