LOCUS       sequence-396339-        5027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains PR1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP56
FEATURES             Location/Qualifiers
     source          1..5027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4613..4632)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4614..4632)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4642..4664)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4858..4877)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4977..4999
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tatatataac gatcattgat tagtatatat
     2281 acatattgta ttgtgtgttt aaagtaaatt gtgtcctata caaagaatat ctttgtggag
     2341 aagcaaagag aatacatact tacgtaggaa tctttttgtt ttcttttttc aaaacgtaag
     2401 aatgtttgct tccttacaat tcatacttat taacttacat attatgtttt cttttaaata
     2461 ttaaaaataa ctaattttta ttaggcagca agtcatttac aaagtaaaaa atttctccat
     2521 gcatgtaacc ttcatttatc attcatttta gtttgtaact ttttattaga ttttgatcaa
     2581 gttaaccgct aaaatctcat tttatccgtt cgcattaaag ttaaatagat tgctgacata
     2641 ttttaaatct aatagaaaat gccatctggc aaataaacaa cggacacgat tttaaactaa
     2701 attttaccaa aaagaaaaaa cttatacgac ttttcttgct tagaagtctt tgcattgtta
     2761 atagattgtt gaaaaggttt attcattact ttcatgcaga gagataacat atcatcgcgt
     2821 ggggatttat tcaatccaaa gaaaagcttc caaaaactga ctttgcttca tgaaacactc
     2881 actctaattt gcttcatcaa tcttaggact gacttttcca aatcaatatg cgaactatct
     2941 tctaatttac attggtttcg tgttttttcg aaaggagaca actatctttt taaaagcttt
     3001 tctatagtgt gatgacaaaa aaaaaatgta attgttagtt gcaaaagaaa agtacaatag
     3061 tcttttctag ttttgagagt ttaaggttta tgatcggaac ttagagttta aatttaaact
     3121 attttgttaa tttttggact gataacagtt tttttttgaa aatattgaaa cgttgtttac
     3181 ctaatgtaac atgttattct acttaaatta ctttatattt taataacata taatattgaa
     3241 taggatatca taggatatta ttacgtaata atatcctatg gtgtcatttt ataagttagc
     3301 acaagcttgt tttaacttat aaaatgattc tccctccata taaaaaagtt tgattttata
     3361 gaatgtttat accgattaaa aaaataataa tgcttagtta taaattacta tttattcatg
     3421 ctaaactatt tctcgtaact attaaccaat agtaattcat caaattttaa aattctcaat
     3481 taattgattc ttgaaattca taacctttta atattgattg ataaaaatat acataaactc
     3541 aatcttttta atacaaaaaa actttaaaaa atcaattttt ctgattcgga gggagtatat
     3601 gttattgctt agaatcacag attcatatca ggattggaaa attttaaagc cagtgcatat
     3661 cagtagtcaa aattggtaaa tgatatacga aggcggtaca aaattaggta tactgaagat
     3721 agaagaacac aaaagtagat cggtcaccta gagtttttca atttaaactg cgtattagtg
     3781 tttggaaaaa aaaaacaaag tgtatacaat gtcaatcggt gatctttttt tttttttttt
     3841 tttttttttt ttttttttgg ataaatctca atgggtgatc tattgactgt ttctctacgt
     3901 cactatttta cttacgtcat agatgtggcg gcatatattc ttcaggactt ttcagccata
     3961 ggcaagagtg atagagatac tcatatgcat gaaacactaa gaaacaaata attcttgact
     4021 ttttttcttt tatttgaaaa ttgactgtag atataaactt ttattttttc tgactgtaaa
     4081 tataatctta attgccaaac tgtccgatac gatttttctg tattatttac aggaagatat
     4141 cttcaaaaca ttttgaatga agtaatatat gaaattcaaa tttgaaatag aagacttaaa
     4201 ttagaatcat gaagaaaaaa aaaacacaaa acaactgaat gacatgaaac aactatatac
     4261 aatgtttctt aataaacttc atttagggta tacttacata tatactaaaa aaatatatca
     4321 acaatggcaa agctaccgat acgaaacaat attaggaaaa atgtgtgtaa ggacaagatt
     4381 gacaaaaaaa tagttacgaa aacaacttct attcatttgg acaattgcaa tgaatattac
     4441 taaaatactc acacatggac catgtattta caaaaacgtg agatctatag ttaacaaaaa
     4501 aaaaaagaaa aaaatagttt tcaaatctct atataagcga tgtttacgaa ccccaaaatc
     4561 ataacacaac aataaccatt atcaacttag aaaacatgag accgaattct cgccctatag
     4621 tgagtcgtat tacctggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4681 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4741 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     4801 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     4861 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     4921 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     4981 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga
//
