LOCUS       sequence-396341-        5659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains EDS16 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP53
FEATURES             Location/Qualifiers
     source          1..5659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5245..5264)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5246..5264)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5274..5296)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5490..5509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5609..5631
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgactgcaga gatcaatttt cttttatttt
     2281 attaaaagaa attaatcatg aatacaggat attatattgt tagaaaaata aagaaaattg
     2341 atttctgcag aagaatgata ttcgttatag ttttacaaac aaaatcatat tttaataatt
     2401 agatttagcc agaacggact tggggttttc atatcacctt gcaaccagaa gttattaaaa
     2461 aaaaagaagc aaatttgctt tgtaaactca tacgtattac tttttgtcgc atatgatata
     2521 tgattgatcg ccactccaaa ttatttttgt ctgaattgca gcttctgccg cgtaaggtaa
     2581 gataatcaat ctagcattgc ctatttgttg tattttttat tctgaccagt gaccagcgat
     2641 caaaagtatg ggatttgggc aacttgtata gtagtttaat tggacttatt tcgcaagttg
     2701 tcaaacaatg tctcgagttc aacccttgta gtcgatcctc cttgtgatag aagtttattg
     2761 gtcttatgtc ttaagttgtc aaataaatat ggagtgtgaa catttgaatt ataggtttag
     2821 tcgtagagta attatatttt aacctcattt tctaacttgt gtgtactttc tgttaactct
     2881 tgatttatgt gtttatcatt tttttaagtt atgtgttttc cattatgctc tgtttatcta
     2941 caaatagtag tagctgaata accatttttg tttttaaaaa aaaatcaaag ttggacttcc
     3001 aagtcccaac taaagattaa aacatgcatg caaataattt ttgcaagtag gattttcttt
     3061 cttaggaact cagactccag aagacgactt tatttttccc aaattgtttt atactcaaag
     3121 gaataaatta tccacgcttt gtcacacaaa aaaaaataaa aattatatag atatatagat
     3181 atatacatgc aggaatttaa ataaaattat taatcttacg taattaagaa cttttttatt
     3241 tttttttaaa aatcgtaatt aagaacttga atggtaaaat actagaagaa aattgttagc
     3301 gtcagtgtga aagcaaatta tactgtaggt taacttttct atagtgagca aaatacatta
     3361 ggttatctaa attcaactaa cgtcctattt ctagttattt gactaaccaa atcgattttt
     3421 tcgtgaagga aaaaaaaagt gtttagtaca ctcatatagt catattctta catatagaat
     3481 tcaagatttc gaactaaagc atacgacact agacttaagt ctagaaggat gacggaaatg
     3541 agaataagct aaaacgtttc tacgaaatta agaaattcgt agcatccaca acacacatat
     3601 tcaaagtctt gaataacaat aaaattatgt tcagctgaga gtctgacacc gcgtccaaca
     3661 ttttaaaaca cttttaaaag tcatactata atatctgagg accgtgtcta gtttcagttt
     3721 attcggtttt acaacagcaa tgatgcttgt tgaattatgg tttcattcta ttggattatc
     3781 tgcaagactt cttaatttta atatatagct cgtgatgata aaataacaat ttgacttcta
     3841 aagtctagtc ctttatagtc ttaacaatat tcattttgac caggttgagt aaatcagaca
     3901 aaaatctaaa gacaaagaat aatcgtttcc tcaaatatgt acttggtgag ccgtcttaat
     3961 caccatatgt acatgagaat aaatgtgtaa tttggtggtt atttcataga aattttgggg
     4021 aaattgttgc acctctccac tctttgccag tctcgtgtga tcttaattga ctcaaaatga
     4081 aagaaaaaaa aacatgaaat taaagcaaat attcctattt gaattatgtt cctttaagat
     4141 ttttaacaat attttttaaa tgataaaaac tggtctgaaa gagcctaagt gggtttccta
     4201 gcaaaaaaag atactcaaag taatttactc ctcaaaataa tgcaatgatc taatgaagat
     4261 ctgttcaaat agtattgatt ttccaaattt aattatactc aaagagtttt aaaatctgtt
     4321 cttgttattc tatgctttgt tttacatgta aagtacttaa attcatttga tatttgttat
     4381 tactataatt ttagaaatgt gtcaaaattt aaaggagcat gcgtgtaatg ccatatgcct
     4441 tatgtacgag aacttgtaat gcgtttgcaa tttgtagtga catgtaatgt tttttacgat
     4501 agtttaaagt gcaaaccgct tccgtatcaa acgagaagag tcgtctagca tacaacaccc
     4561 acacgagagg aaccgcttag aaagagaatc cacttgaaat tgctgtccat gcattgctca
     4621 aattcattga tacttggtct atgcaatttt acactacctc atttctgact atttcaattt
     4681 gtcaagatat tataacatgc atttataact tttttgtcga cgataaatca accaaacgaa
     4741 tccggtctgt atgtttgata acatgcattc atacaaaacg attatctgtt ttttagtcta
     4801 tttaatttgg tttctacttt ttacttttgt gaaactaata ataatagatc aaacaattaa
     4861 ctgatataat ttattaattt aattcttaga ccaagtaaat gattcatgaa atattaaatg
     4921 cacgactaac tttagaaaaa tgtttctttg tatacataaa gatagaatag aagaaaagta
     4981 ggattagaag aaaaaaacga aaatttcaga gtagtttact aaagaaattc tgagaaattt
     5041 tgtgtgaaaa tgaaatgaaa atcttcaatt ttagtgggcc cctgctacat cagtccccta
     5101 tttatatctc ttctcaactc taaacccaaa ccaaaaaaaa acagaaagag caatctctct
     5161 atactacaaa caccaaagtt taaaattaaa caaaatcttc aagaaacact ttacgaattt
     5221 ctgcaacatg agaccgaatt ctcgccctat agtgagtcgt attacctggc cgtcgtttta
     5281 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     5341 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5401 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     5461 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     5521 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     5581 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5641 tcatcaccga aacgcgcga
//
