LOCUS       sequence-396345-        5172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains GLR2.5 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP29
FEATURES             Location/Qualifiers
     source          1..5172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4758..4777)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4759..4777)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4787..4809)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5003..5022)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5122..5144
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgataagaag tgattcgctg gggtttttga
     2281 gaattttagg acaagttttg agaatatttt tctgtctttg gcatgaaaag tgtgtagttg
     2341 ttctatggag ggtggtgaca tgatcgatct cggattcgaa gagactattg ggaagaaaaa
     2401 aactagattg cgaaaagatt gaaactgtga aaaattgata tgtagattgt tggaatataa
     2461 ttgtccctat cagattggtt tgtttttata cacatatgag ggtcacatga tttagaaggt
     2521 tatttaagat aataaatgta aagagttaat tttcgtatcc aaccacaaac tagattttat
     2581 tgatagtttc aaagtaacag ttactaaaga aaagtttaag aaatgaactt ttttttctaa
     2641 gtttgttctg ttacatgtaa attttacacc taatgacaag cttctcttgg agtttagttg
     2701 actattgaca ttgaatttta atgacaataa aatttaaaag taaaataaaa atgagtttat
     2761 tggtcaaacg tttttagcaa atcattattt actaaatgag tcttaactat aattagactc
     2821 taaagttgtt tcctaattaa ggtggtcaat aagaaattgt attttaaaac tatttaatgg
     2881 ttgagctcta aataaaattt gatgacaaaa aaaaagaaaa aaactattta atggtgtcat
     2941 ttccttctat cctaatcttt tttttttaaa tctaattggg gattatttaa tcttttcatt
     3001 tatttataat attaatcatt accatttata tgttactcaa attggtaaag aataattaaa
     3061 attttcaaca attgtatatc caccaattta caagttctta agccagaaca tattttccct
     3121 aataaacaaa ttttgaatac attgtctaaa aaaaattgtc acagtaattt cttaagaaaa
     3181 ctaaaaagac tattctacat attaacaaaa aaaaaatcat gtaaatattg tacgttgaaa
     3241 agagaaaacc tctctcaccc tggtcgcaaa gaaaatcctt taaaatcctt gaaaatctta
     3301 aaaacttgtt ggataaatag tagatcttcg atgaaaatat ggacatgtag aagattttag
     3361 cagaaaatcc tttttgtagt ttttggaaca attttcacct actggcagct tttaccacga
     3421 cctgaatttt ttcatcgact ggctttacca caagatgagg ccaaattctc caactttttg
     3481 taaaaaggat tgcaactctt tttataaagg cttaaatttt attttatttt tgctttagac
     3541 atcaatttat gttgggcctg ccttgacgtg ttggctaaca aaccatgaac tctaaacctc
     3601 aaactgtaca gtctctttaa acccttttga tacaaaatct caacagcaac gagaatatat
     3661 attagttccc aagaatacat gaacgatccc atgaagaaga ttatacccaa cacgtgttac
     3721 acgtctatgt tttcagttcg gagtatgaaa ttgtgtgaca ttatggtatc atcagaatga
     3781 aaatttaaac atacttttaa gcgtaataat atcctcaaag gttttagcaa aaaataacat
     3841 tgtaggtgtc aaatccaaca tagaaacaca atatagatat acgaaaaata atctaagtta
     3901 acgcgctcaa tgaagaatgt gaaaatttaa atgaacgcaa atacacaatc atgaaatttt
     3961 ttgtttaaag aacaaaaaaa aagtgattac gaataaaagc aagtcaaatg ttcaacttag
     4021 tcatgtaaag actttttaat aatcttcggt caactaggtt ttcttccacc tatttctacg
     4081 tgtggtgtga cacgaagtcg gccgtatgat ttaataggtt ttaaagaaaa agaaaaaaat
     4141 aaactcacct ctaatctttt tttttctcgc aaactaacct ctaatataca ttattctttc
     4201 tgaaaattat atacattttt aaaaaatatt tgatttttta attcgttcta gtcaaattta
     4261 aatgttggct taattaagca aagctagtca cttagttgat atttagtatc accttaataa
     4321 taataatgta atgtttttgg aagaccacgt attttcctca taaggcctgg ataacgcgtt
     4381 acttctccaa aatgaccaaa aaaagtcttc tctaaggtat gttattaaat catttaaatt
     4441 aatatagtct acttgcaaat tccgtcacgt aattagactg aattggttat cttcttcaaa
     4501 actatcattt tatttgaccc gtagtctctc cgatcccctt gtagttatga aatcatcata
     4561 gttatgtctc ttttccatca tctcgtgtct cgttttcttt cattatggct tttaattttc
     4621 ctcgtcttct tagtattatc actcgggaaa tctcaaaagg aagctttaca agttaaagtg
     4681 gggattgttc ttggcagtaa tgtgacatta gcagatttga gcttgcgagc tatcaataac
     4741 atgagaccga attctcgccc tatagtgagt cgtattacct ggccgtcgtt ttacaacgtc
     4801 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     4861 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     4921 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4981 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     5041 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5101 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5161 cgaaacgcgc ga
//
