LOCUS       sequence-396346-        5184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains WRKY53 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP43
FEATURES             Location/Qualifiers
     source          1..5184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4770..4789)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4771..4789)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4799..4821)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5015..5034)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5134..5156
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tatcttgtga gctgattcaa agatttcaag
     2281 agttatgttt tggatgcaaa tatgtcaaat tacataattt cagctgattc tatgaagtgt
     2341 aaatacaaat ttagggtgca aagtgccaag attcatttta taaggatgaa tgatgaatga
     2401 gatccaaata ataataataa tacaatgaat gaatgacgca aaagagagtg acgccattat
     2461 aaaaattaaa atatcatttg gagtactaaa actagaaaac aaaaaagaat gataaaaggg
     2521 aaaatccacg cggagagagg tttgaaatgt cttcggtcca atcctgtcct tttgaatatt
     2581 gattttcttt ttgatttttc ttgcttttta ttaatcaaca aatctgtttt ttggaattac
     2641 gttttttcta cgttgaagtg attttttttc cttttgtaaa aggcctacgt tgaagtaaaa
     2701 ttacataaat aaatgtatgt tatgaatatc gacccaaaaa acaaattatt tctacactat
     2761 actttgcgca aatcaaatct atcgaaaacg attttggtaa tcccattccc atgagtgagt
     2821 tttgacccaa aacaaattta aaaaagtaac catttcatta gtaaaacggt aaatatggtt
     2881 cataaacctt ttagtggtta ttggtcctgt acactacact cgtgtgtgtc aacttcagct
     2941 atgagatttt tgctgtaact ttttggataa cggttcaatt attacaaagg ttatttctta
     3001 cgtatgagag gtccaaatta caattgaata atatttgatg acatacaaag ttaaaaagga
     3061 ttaatcataa gaaatgaatg ttggaatgga atgcatctat cgatctgact actttagata
     3121 gcttgatgtt ggacttagtc agcaaagacc ttatcatact cttgttgtat tgtatatatc
     3181 gtatcacaca gtgactggtt ttttctactt ttctctttgg aacatatcaa tcattatgta
     3241 taaataaatt ttgtccaaat taatggccat ttgtctcaac cataaataaa tatttattca
     3301 cacacctata tgactatttg agataattcc atggtgactt ggtcagcaaa aaccttgtca
     3361 tactcttggt gtattatcac ataactttgt agattatatt cttatggaac atatcatata
     3421 tatttgtcca aattaaaccc atttgtatta ttttcccctc tcgagtttaa tatttcttat
     3481 ttagaaacag ttaacaactt gacatagctc acataatcac tcacagacta tatatgtata
     3541 agcaactacc attttaaata ttatttagtt atattgttag actgcaacta ttatttattt
     3601 tctttgccaa acaaatttat attttttcat gttaatacaa tattatatct tgaacaaatg
     3661 tatgcttaaa tatattgaca caaaacaaaa acatataggg gtttttagga agaaattttg
     3721 tacagattaa ttaatcctca tcagattttt attttatatc ttatttttag aagtgtatga
     3781 ttttaaaact agaacaatca aatcttagta aatttcattc aacataattt ttatgttatt
     3841 gctaatctat tgaatgttaa taagaattgt attataccaa attaccaata tgtgttttga
     3901 tacttgtact taaaacagaa tgtggaatat cctttccctt attaatgata ttaaacatca
     3961 ttacaattca tagttatagc aatgaaatat tatccataga aaggttgttg cataatgcat
     4021 actagtaaag catcaaccaa catattttga aaatattctt aatcaaataa aaaacatata
     4081 ttggaaattc tttaacagta gaaaaaaaca tataataata gagaatctta atcacatgta
     4141 caaagtcgta gttttgaaag tgtacattga aagagacagt tgtataaccc tttgttgtct
     4201 aattatataa ttaacccact atagttaaat aaatgataaa cttggttaca actacattaa
     4261 caaaattgaa atgttgaatt ggtcagcctt tgaccttata ctctttcact accaccaccc
     4321 cacttatcaa ttacactctg gccctatact tccttatacc aaagactttt cttcaatttg
     4381 aaagttttgt ctctctttta aaaagaaata aaataattat ttatggtttg gtcatttggt
     4441 caaattttca aacactgaaa atccaatgcc tttatatacc aaaccaacac tcatccattt
     4501 ttatcaccaa tcctataaca ctctcattct catcatatca ttcttcaatc tatataaccc
     4561 attcttaatt atactcaaca cacattatat ttttctgatc atatcattct ttcagtccat
     4621 ctatataacc aattcttgat ttatacttaa aacacacatt atacatcttt ctcatcatag
     4681 tttgtatcaa tttcctagag taaactacct aaaggaaaaa aaaaatctat tttgggaatc
     4741 atatactaaa acatgagacc gaattctcgc cctatagtga gtcgtattac ctggccgtcg
     4801 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4861 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4921 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     4981 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5041 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5101 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5161 caccgtcatc accgaaacgc gcga
//
