LOCUS       sequence-396347-        6686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains GLR2.7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP30
FEATURES             Location/Qualifiers
     source          1..6686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6272..6291)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6273..6291)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6301..6323)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6517..6536)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6636..6658
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tcacacactg gtcacttaat ggtttatttg
     2281 actggtcaaa tacttctaat ttctaaatta atttatttta aatatgtttt gtcagaaact
     2341 tcacctttga tttgaccact attattacag atcatgacca tgattataag aatattaaaa
     2401 ttaatgaata ttaaaaaaaa aacattaatg aatatttttc tcagtagact aaacaaatat
     2461 ttactggtct tgtgaaaata gtaattttag atattctaag gccacgaaga aatagaaaat
     2521 tttaaaatat atgctaaatt tttttgtgat caattaattt aagatttgag atcagctggc
     2581 gccacacgca aaataaccaa tttgattcat tctttgtgtg taaacttttt ttccatcttt
     2641 gaacgtaaag ttgttgtcta ttttgttttc ttaatgaggc aaagctcaat acccatcgtc
     2701 gatctaagat tccctcttgg atccacataa tgatcactaa attttatatt ttaatcaaag
     2761 cattaaacaa tgttgatgtt aaataataaa attgagccat taacatgtgt attaagttgt
     2821 ttttgtttat gggagagtaa catgaaagcg tattataata atacgaatca actaatgtaa
     2881 cgttgtaact ttcataatta gtttaagctc attttaattt ttaattttta cgtataggat
     2941 aattggtttg attagacata tcgcaaaagt gaaaaggccc aaataattaa aataagagtg
     3001 tttataccac tggaaaaagg tcatgtgaga ttgtccttaa attactaaaa aagaaagggg
     3061 gcactgaaaa taatttactt tagaattatt ttgataatat ttactggctg agacttgaga
     3121 gttgagagat cagagactct gaccaatggg aaattctttg gatgggtcaa acgttgtgcg
     3181 ccactaagca aaataaactc atatataatg ttatccgttg catacaattg atagatttat
     3241 gttattacaa agcaaatttt ccaatattct ccaaactttt gagaagaaga aaagcaagtt
     3301 tcaaacgttt cttaaatcgt gagaagaaga aaagcaagtt tcaaacgttt cttaaatcgt
     3361 aactcaatgt aagtatgtaa caaaattaca caaacaactt ttctttggta tatacccaag
     3421 tataaagtgt ctaacaaatt ttactatccg tacttaaatt tgtgattaaa catactttgt
     3481 tcatcaaagt ttcttgtgca aatcttttta agttgttaaa gtgttgaaat ggacttgacg
     3541 tagtaaaact ggtatatgcg tatagccaaa ggattttttt acttttatta tacgtgtcaa
     3601 ttagtaaaca atgcaacacg agtatcaaaa agagagatat tgtgtgcata agaaggtgcg
     3661 ttcagatgtt gaagctagta ctatgttggg tataagaaat taaattacag cttagaatct
     3721 aagcctttga tgttttaaaa aaagaattgt taacttctct tcttttttgt acgagagagt
     3781 aattttatat aaggaaaaat atggtaaaat gggattagaa gaagtggtaa ggtcacatgg
     3841 aaacatgtgt aagctgagta ggacatggtc aggtcggctg gtgggtctta atttttaacg
     3901 caaagcccca ccattttcac ttcatttatg taccctaata aataattcac gtaatccttt
     3961 tgaagttctg ttttggaacc aatgatttgg aatttatgaa aagaatcgag ttcaacgaaa
     4021 catgtaagag tatttttgtg gattttctct ctaataccgt tttctttttt tttttgtcac
     4081 atcatccata tgtttatttg tgaaatttcg ataaacgatt agatagtggc tgtatatgat
     4141 tttattataa gagacttccg caatgttgca aggaaaagta ttctaaaaaa aaaaagtagt
     4201 actctaaata ctttctgata taacttaaga cttttgttaa aaatttcaaa ctcaaaatga
     4261 tgcgtatcat taattctata gttttaagaa gagaaaatat aggatatatt taagaagaca
     4321 aaaatgttta catacacaaa tgtgattgta aaagtcacat cttactcaaa aagtaataaa
     4381 aaataaaata aaataaaaat atatggagaa aaaggacacg aatctatata aatagatgca
     4441 acttttttaa agggggattg acaaaaaggc actaaaaaaa ctctcctttt gacgggcgat
     4501 gtctcaaggg aaatcttgaa tgtgactcaa agcgaagaga tgaaacagct cgaaaagatt
     4561 tggtttgaga atcaaaattg tcctcataca gacaacattg tcgtcccacc gtctcaacct
     4621 taatagcttc tggggtctgt ttttaatagc aggccttgct tcagtcatgg cacatctcgt
     4681 cttcatagcc aacttcttgt acgaacatag gcacacgcta aatgaggaat cgaaaatttc
     4741 cttttccaga aagctaacat ttttgcttag aaactttgat gaaaacgaca gaaaatcgca
     4801 catgttcaag gagagtgcaa ttcataatgt gagttcacct tttagtcaaa ccactccaag
     4861 accttcaaca gtgcagatca caccatgacc acgaagtccg tcgcaaaata tgcagtttga
     4921 gccaagatgt gtatcttttg aaagcaaaga atttttcact ccccaattag aatgtatagc
     4981 taaatgataa atagaatttc aaaagttgtt gaagcatttt gattacttag ctaattttac
     5041 tgacttattt tcatattttt gtgtgttagt ctcttctcaa atctatatat aacccatgat
     5101 atactctata tttagcactt tatcatggtt attaagatcc attttatggt gtttcattag
     5161 tattttcatt gtggtttgtt gcatttagtg tccaagtgag tcttcttgca ttttagaagt
     5221 cctatagtta ctgtgtgtat tttgtgtctc ttttgagata tttcatgtta aaataatgga
     5281 ggacacacat ttaaatgcaa agaagagaat tgatgattca taacctatct gaagtctctc
     5341 aaagatcgac tcggatgaag ttctttcatt gagatttagc tttggacctc tttataatgg
     5401 ttataggaaa ttgaaataga tttccaatgg aggtgatgtt tcatgggtat gtgagctatt
     5461 ggtagagatt tatggctgat tttcgtatac atatgtcaat ctatcgcaga aaagaaactc
     5521 atctagaaac ctctcttaaa aacggacaga agcttcttcg acctccacct tcggccccaa
     5581 caccaccaaa gcccctctcc gatcaccatc gtcgtccatg acacaaccga agccatctct
     5641 ttttagcttt gatccaaatt gcaaccgggt tgtaacccgg tttatctcgt cgatccggtc
     5701 agaacctatt aagcaaattt tagacatttg ttaataataa aattttcact acaaaaaaaa
     5761 taatatagta attttccaaa aaggtttttg gttcgtgtta cattttctga gatgatccat
     5821 caacttacaa ttggcatttt ctttctctct ctctcttagt aggatattca ctagcgtaaa
     5881 atcactcgat tatgaccttt tatgtgtatg aaacggtctt gctagctgga agaaaaatat
     5941 taatcttccg tcaaacaagt aagaagactg aagttggaga tttttcaaag taaacgtatg
     6001 tagcctaaaa aaaaggagtg acaatggtag agaataatga caaaaagcga gctcatatta
     6061 tcgtccatac aatttttttc tgtcactttc aaaccttaac ttcgtttgtt ttcttttaca
     6121 ttcttgtttt catattttat tttatctcta tatatgtata tatatatatg tatgtgtgtg
     6181 tgtgtatata tgcaatagaa caataaaaaa gaagaggaag ttaaagaatg acataaagtt
     6241 cctcaatctg gaacatgaga ccgaattctc gccctatagt gagtcgtatt acctggccgt
     6301 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6361 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6421 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     6481 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     6541 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     6601 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     6661 ttcaccgtca tcaccgaaac gcgcga
//
