LOCUS       sequence-396350-        5726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains MLO6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP47
FEATURES             Location/Qualifiers
     source          1..5726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5312..5331)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5313..5331)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5341..5363)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5557..5576)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5676..5698
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc taaatataca tttggttgac atgtttctca
     2281 ttggagtatt tggttatttg tatacactct ttacatcctc atattcatct gttaatttgg
     2341 ctttgtaatt gattgtaatc acatcgtgtt atggtcaatc atatataaat attttaacga
     2401 tatatcgagc tatttttcaa tttcttatag acatttgagc gattatcata cttcttgcca
     2461 caataacaag aactatggga atatacaata actattaatc cctaggatgg aacttggaag
     2521 aggtttcact ttttgatttc actttcaaca aatgcgtgtg gccacataaa cttttgagct
     2581 ttgacccaaa tgttttatct aaagtgggct tgccaaaaag ctcttgttaa aagacttgat
     2641 cacctctaga ttaatagggc ttgactcgga catttgtgcc aataggaaac aaaaatggat
     2701 aaccaaaaag aataccaaaa gctgttagat agaataggaa aaagtgttaa gtgatcatca
     2761 gtacaacaaa tgtcacatct aataaacaag taacaaagta ctacacggtt catttacaaa
     2821 tgagcttgtt tgcaatcttc aaggaaaaag tagtatgcag caaatttctt ccaagcagga
     2881 acattttgtt tgttttcgcg atttgcagta gcgaacagaa ttgaccatgc acgtttgtat
     2941 tttgtaagat tgacgttgag atcacagccc accgggactg gtgagattaa agcatgttag
     3001 agtatgttag agttttgtaa ggtaagtacg accggataat acacatattg aattgctatt
     3061 tgtcattaat aaatgtcatc acgacagtcg aaataacatc atttgtagta gacatgtatg
     3121 ttgtcgtttg ttacaatatt ttcttaccag attagtattc attatagacg tcaatgcata
     3181 aaattacaga tgtacgaacg tatatgtagt accatgtgca tggttatatc aatattcgtt
     3241 ttaggtttgt tagaatgcaa attaatcgtt gtagagccat gtccgcattg cattgaaaaa
     3301 tgtaagtcta taacacagtt ttacatgaca tgttaatgac tagggtttgc caaaatatat
     3361 aaatacttga tttgattagt tatatgtccg aatctgtttt aaattatcat acgcgcgcca
     3421 attacaaatt gtttatcaca atatacaaac tacaacacca tatttcatta tagtaaaacc
     3481 aaaatttaac tacgaatctg aacgtagccc tgctttatga taaatatttc ccgaaaaaaa
     3541 atcattagaa ttcctataat gataaaaaaa acgctggttc ttatggtaac accatgtgaa
     3601 aagtgatgac tttttcatag cagtagaagt agtcaactac ctcagataag tactgtaatt
     3661 aaataaacta attgcccaaa atgatacaac tttgtcgcat taaattaaaa gttaaacctt
     3721 cactggatgc attaccaaaa tggtcatata acaatacgta atccaactcg ctgtagctat
     3781 caatctcttt atcacaacca accaatattc aacaatgttg ttttatttat ttttcatatg
     3841 gtttatttaa tcacctcgac ttttttggca tcgatcacat ctacaaataa attgttagtt
     3901 agcacgaccg ttattattac tttatgaaga attgttaatc tgaaaaatat ccttttgata
     3961 tcagttggaa atattttttt aaataattta ttatgtagag cttgcggctg agtcaaataa
     4021 aaataaaatt gaaaactaaa agcgtgattg acaaaatgac tttaaaggct gatttgtttt
     4081 tgaaaaaacc aagttcttaa gaacacattt gaaccaatca tcgaaatata tggtggaaat
     4141 atatttctac caaagatttg ttttttaacc aaataattga acacaacgct aactgaatat
     4201 tttataaaac tgatacagat ttattaaaaa ttttgctaat aaacattata aaattgcttt
     4261 taccaattat aggatgtatt gtatctcttg gactttaata tcgttgacga tcttgacaaa
     4321 taaaaagctg gcgtttcatt aaattggtat ttacaatgag aaaaatgtgt gggatccaaa
     4381 actggatata ggattcgctt tactgtatct ggatccgaaa ctctaaaata tgatgtttct
     4441 tatgatctgg attttttcaa actataaatg atttctgaat ttccgtgtat gtcaaccaat
     4501 atttaaaaac agattatatt tcttaaatat ttttcagatt ttttgaaaaa tattctgtaa
     4561 attacaaatg caaataaatt atttaagacc gttaaggatc aaatatgttt ttagtttatt
     4621 caaatctctc gttctctcat atcgctattt tgtacttagt ttagataaaa gtattaagtt
     4681 tggcttctta gaatttgatg tttctttttt tcgtttttgc tatcgacttt tgtttttttt
     4741 ttttttacta ttttgttgtt atttatctat tccttagatt ttggttttgt ttctgatact
     4801 tatattttct gtaatttatg tcgcaacttc aaaaataata taaatacttt acattgatat
     4861 taaaaaaaaa aaattgagct ctaatgacct ttggagctca tgctcatcta tggaaaaaaa
     4921 aatccatgca ggaagaacca agaggaaacg gtatacaaaa taatattaaa taaataatta
     4981 tttgtcaata aaataaataa aaactcacca aagtacataa cagttcacac agcatgtttt
     5041 tagaaaagat catatactat tggtttcaaa gtctttgact ttgaatgttt gaacttttca
     5101 aggttcgcct actcgccggt cgtctccttt aaccttcgtc tctgtgttta tataagaaca
     5161 tatacgtatt tgatgattac aaaaagacaa ttctttatcc ttctttagat ttctgtggaa
     5221 agttcactat ttattaggag agacaatttc aaaaaggaaa gctttttgct tggaactgtt
     5281 ctgtgagttc taacatgaga ccgaattctc gccctatagt gagtcgtatt acctggccgt
     5341 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     5401 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5461 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     5521 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     5581 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     5641 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     5701 ttcaccgtca tcaccgaaac gcgcga
//
