LOCUS       sequence-396351-        7301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains MYB72 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP59
FEATURES             Location/Qualifiers
     source          1..7301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(6073..6090)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6887..6906)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6888..6906)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6916..6938)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7132..7151)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7251..7273
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tacacgatct cttttgagat ttaagaagtt
     2281 tctagtgtct tatagtatct tgctagctat aggggaaaca gagttatgta aattacatat
     2341 tctttatcta attggtttat cagatctatt tgaagatggg attatatata taagtgaaat
     2401 aatcgagatt taaggctttt atttttctgg ttttcatcaa cacatgcatg aagaatttgg
     2461 tttggagaaa ccgccgcaag atgtttccca taagtatggc aacagtatca ttagcttctt
     2521 cccaaacttg ggggaataga taacctcaca aaatcaatag ataagctcac agaaaaacaa
     2581 aatgtgccac tctttttttt tttttttcca gataagtaag tggcagggag tatgtatgat
     2641 cccatgttac ttcacctcca caaccacatt cgtagtcacc catatttatt agcttttgta
     2701 tttatttttt tcattttcta acaaaggatc agaaaccgat catacagcta attttatgtg
     2761 attgtgaata attagagttt tttttattat gaatctagat atatacaaca atcaatcatg
     2821 attaaaacca tactatacaa attcgaactt ttgcttacaa aaaaaaaaaa gattcgaaca
     2881 tggttcatca atcggatggt gaattttatg accataagaa taacaagaaa aatcttacaa
     2941 acccctcatt tattacgttt tctatgtttt taattaaata aattatgttc tgctattaaa
     3001 ttcccaaaat cgaactacaa gaaagtaata aaaacattat gattattgca ttcccaaacc
     3061 atacattagt gcttctctgt ttcgcattgt caaagtcacc atggtccaca cggcgattga
     3121 aatctacaat atacaattat gttagaatca gaatgaaaat aattgattta ttctaaactt
     3181 ccaaagtttg aataaaatca caataaaaat tttaactgct agattagcat aaaatatgaa
     3241 atactaattc atggtaactg tggatgtcgg aatccacaat cagaattttg tggttgtgtt
     3301 tcgaggatct taagacttgt taaacgaaca aaaatcaatc ggaaaagtgt ttgtttattt
     3361 ctgtatagtt tcagtgtcaa atcccctttt ttttctacaa tcataaacga aacatattta
     3421 tacaagcact tcgaatcacg tgaattaaac gtcgagctga tctctcgccg ggaatctcgc
     3481 tccatttact tcggacgtta tgatccgcca cctcgagcaa cttaattagt gtctttaatt
     3541 cgtgattctt ccgttacttg aattaatagc gaatcgaatt ttgaactgac gggcttctta
     3601 atgggcttga ccttcctgat gggctctcta cagaacccag acctcacaat gggccggata
     3661 cggaaattga catccacagt agccacatgt acttacgatt cattggtagt cttttggttc
     3721 atcttggtat attttacata acatattcca aaagacacat ttacttcatg agtagccaca
     3781 tgtactgaga tcctttgcaa ttttattatt tttgacatca atgtaagtgt ttatactatt
     3841 tttgcagttt taacagaaat tacaaagaat aaagaatata taagactaaa agttacagaa
     3901 acacaacgca atacaaacag aaacagaaca aaattacaac gaaaacttca caagcataga
     3961 ttctttgtaa aattgtacct tctatgtcta ttgttgttgt tttctatgta gtttattgct
     4021 tttgaaatat aaacttgttt tctatctagt tatgtcgagg agatcaagac catctttttt
     4081 ccagtaatct tatattgtgc tactattttt tatttttcct tcgacaaaac aaaacgaaga
     4141 gccccatatt ctccgacatt ctcgtttgga ccaattcttt cacactacca tatccaacct
     4201 caagactatc ataaagatgc tattacagac gactatttct gaacatgtgt aagcaactag
     4261 ctagtgctcg gttccttaag aaccaagcca gaacctgcca taactataat tatagaagtc
     4321 atgctaataa tacgaaatcg ttttccatat tggttgtcca gctttcgggt catccaaatc
     4381 ttttctttcg aaaagcactt ccttattctg tgatttgagt tgaatgtgag gatgataact
     4441 ctttatcctt ttattctcat gctaaattac caactgattg ttttaaaggt tgaaaaataa
     4501 cagattttct taaaaatcac agtgcatata tccagaacgg catttagatt aatcttaatc
     4561 tcgagaatct aatgactttc attctccatt attctacttt tcataaattc ataattacaa
     4621 taaagttggt ggacgatatt ttacgtacgg acaaaattat ccattgtcaa aacacgtgta
     4681 gagttgaagt caggcacacg tagaattgca caagtaacac gacgtcgcga ggaagaagtg
     4741 ctaaacatac agtgactcac aagctcttaa tttccttttt gttttgttta ctttttaatg
     4801 tgaattaagt catacaagtt gaccacttca aataaacaaa ctatttctat tgtatatatc
     4861 tagacgtatg tgatacatat ataatgtgat tgccaatcat tacaagtttt tacttagacc
     4921 cacacatgct acgtcacgtt ttaaaacttt accttatgtc caatctcttt tatcgatatt
     4981 tatttcatat gtatgtataa gatattatat atatatatat aggaccctaa tgtgtttata
     5041 atatatatcc gtatacgtac gtacaaaaga agtacgtatg ctgattttga ttggaagttg
     5101 ggaatggaag catggatatt attcgtactt ccacacattt tattagcact ttagattacc
     5161 tcatattaca tttaacctca ctaaaaggta agagttacga gactcttgct tgcctaacgt
     5221 atgctaaaag gtattcatgt attatgtatt catgtttttg tacatcatgc attaaaccta
     5281 tataaaattg catacttacg tatttatata aagtagatag catatgtatt atacggatgg
     5341 aatgatcatt agtttttttg cgcaatctta catcagagcc ttgtggcgtg gatttgaagt
     5401 ctaaccatgc catgccatat tttaatttat ggaaaacacc aatatataat ttaatcatca
     5461 caacttatag tctaaacaac tacaaaatta atcatcatac atcatgattt ggatgtacaa
     5521 cttcgtgttt aaacttttat tttatttttc tctttcaaat aaggaacttt gcgttctgaa
     5581 gtggtgtttt aaagataagt catttatgaa gtcatttata cgttagatat aaatattttg
     5641 aaattttatg aagtggtgtt ttaaagataa gtcatttatg ttcagtttaa aacgcaatgt
     5701 ctatatatac cagtattctc taattactaa tagaaatgtg aactttcatt attctttgta
     5761 ttaaaacttc atagcaaaaa aaagtaaata atattccctc cacatcaatt taagtgatgt
     5821 tttaagggta ttttgttctt gattcattat aagtgatgtt ttcaatttgt taatatctta
     5881 atttttttgg ctaattatgt tttacatcat gtttttgtta ttgtctgaaa actgaactta
     5941 atgatgtttt tatgtaaaag agataaatta aatgaatgtc ttgtgttttc ttaatttatg
     6001 cgtaaaaacc ttaaacatta gatacaaagt gattaagaag taaataaaag gagtaaataa
     6061 gagtaaactt agatgatgat gatgatgatg tgggttgagt tttataagaa atacgaagac
     6121 gagtaaataa gttaataggc taaagaaaag gattattgaa ttctccttct gaactaaatg
     6181 tcaaataata agtgtcagga ttaataaaaa ataaactgat ctgaattaat taggattaca
     6241 gtctacagat ttattctatt tatttctcca attggtgtta cttttgtttg tggcatggcc
     6301 cttgtggtct ttaacacaca cgcacacaaa caaccgctct aaagtttaca atatactaga
     6361 tttctactcg ctcatatcaa aatctaaagt cgaaaatctt tttgtataaa atatttaaaa
     6421 ctgaataatc aagaaatgta tcaaactatt taaatatttc cgtgatgctc aaattaatat
     6481 acatattttt cgtaactcgt caatgaatca tgcataactt gtttacacaa ttatacttaa
     6541 tggacactat gatctgtaca cgggcatatg agtaacaaaa gaaatttaag agattactag
     6601 ttaaaatctg ccgttaactt ccgtacgttc catgcttttg gctctctctc tacgagtgaa
     6661 gtgcctcaaa taaataagag ttttattgaa tggagtggaa aatctaatct ctaactaata
     6721 attataaaac atcttctgac gtaaagagcc gggttatatc aatgatatat atataggagc
     6781 tcctgataat aatcaaaaac ataacattgc atatatagag aaagatatag tgaaggtagc
     6841 tatagagaag aaagtagtgt aataagaaca tgagaccgaa ttctcgccct atagtgagtc
     6901 gtattacctg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     6961 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     7021 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     7081 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     7141 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     7201 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     7261 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a
//
