LOCUS       sequence-396361-        4591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains PEP1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP01
FEATURES             Location/Qualifiers
     source          1..4591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4177..4196)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4178..4196)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4206..4228)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4422..4441)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4541..4563
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgaagtcaaa aattgagtcg aaaaatccaa
     2281 gaggccaatg agtcagtcat tgctcctttt tttttttact caaacttcta tgaaaaactc
     2341 gtacgtagtt tattttggtt tcctcatttt tcaagacagc aaaattgacc agaatgtata
     2401 tacttttgaa tcggaactaa ttgttgaaga cgtattattc ttgatcaaag catgcatata
     2461 tctagtacaa gttaatctga cttgaatact tgatttgaca atttctacca cactttgttt
     2521 acctagtaat ctaataccat gtgtattgat tgatatagct ttcagctgat ttcaaaatta
     2581 ataattttta ccaagaaatt gctgtgtcgt tttttttttt agttatacgt ctttaaaata
     2641 agtaaattat agtgaaaggt acggttatat tttaaacgga taataattga gttagtttag
     2701 atcaatcaaa cacataaact tcttcgctgg tcttcacgag taaacgccta aaatctagta
     2761 ttaaacaaat tcctacaact gacaagtggg gacctttaaa tacactagat gagtatccac
     2821 ggtaagcgtg ggtttatatt gaaaattgtt aaaaaatatt tacatttata gtttttttgc
     2881 aaatttatat ttattcttta tttatttttg tattgttaat aaaattatat caataatatg
     2941 taattatatt ttatggttat attcatttgt aactcgaaat atcaatttat atatttcatg
     3001 tataaaattg attatttata agattttaat attttcactt catgaataca tataacaaca
     3061 atatatgata ttctgaaaaa attatgagca attatgttac ttgtgtgatt taaaatttgc
     3121 ctaactcaat tataaaccaa atcttgtttt atgtacttca acaacattgt gataggctta
     3181 tgattaaaat ttggattgat atcaactgaa taagatttga aaagtattcg tatgaaaaaa
     3241 taaaaagata acaaaaatta tatgtcattt aagtcaatac aaaaatatag aatgacacat
     3301 gtcatttttt tgtgtgatga tgtgttattc atttggagag agttgaccaa ttttcatata
     3361 tatgatttgt tttataaggg gaacaaaaaa tacaattgtt taattacatt atcgtcgtgg
     3421 ctttatagaa ccaatccaag acaacaaaac aattataaga agtaagagca tcgtatttta
     3481 gtcaatcatt catctttctc ctaattcaat aaacaaactt ttaaccattt gatgtatttt
     3541 ttaaaaatta atgtatttaa aaaataaaaa aaaaatttat attctgaaca tactaatgac
     3601 cataaatatt tagttcgatt gtaatttatt tatttgatcc aaacttccta taagaaatta
     3661 ggggaaatat taaatattca atctatagtc caacattcgt catcggacga cgacgagtga
     3721 actgttttcg tttttgtttg taatgtttat tccagtatat gtaaaatatt agctgagtca
     3781 tcaacctacc taaagccaaa cgccacatgc ttattttcac taataacaac aacaaatcat
     3841 atatatttat aattacgcgt gaatgaatgt cattcttgat tattttttgt caactaatta
     3901 atcaaagaat cagaaaatgg actagttatt cttattcaaa agtcacgatt tatgaattca
     3961 ccaaaaaata aaaaagagtc aagatttatg aaaatgtatt ttatagtaat aaagagtcac
     4021 acccaatacc gagttagccc actataaaag gcaaagatgt ctctctgcgc aatcactcac
     4081 atataaaaaa cagcttcact cctctcacca aaactaatca gattaataaa agttttcctc
     4141 tgtcttatca gatctcaaca tgagaccgaa ttctcgccct atagtgagtc gtattacctg
     4201 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4261 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4321 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     4381 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     4441 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4501 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4561 aggttttcac cgtcatcacc gaaacgcgcg a
//
