LOCUS       sequence-396367-        4632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains PMR4 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP72
FEATURES             Location/Qualifiers
     source          1..4632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4218..4237)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4219..4237)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4247..4269)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4463..4482)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4582..4604
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgctcgatgt cgatttgaga cgtagttcgt
     2281 gtgttgtgct tgtataattt ccactgaaaa gaacgttttc taaaagttca tgaccataac
     2341 acgtaaagag gcgaatgaag gtaatattaa tgtgggacat agtgttttgc gtgatccaaa
     2401 ttattttagt atcgcaaacc ggcctgtctt aaatggacat ttgtagtaac aaaaccgacc
     2461 atcagcaaat taacgttaac gttgctctaa tcgtgtgtat ttttctaatg cttggtgaag
     2521 cacatgcgac catttgttgt gtcagaacaa aaggaagatt ccacatttag aatttagatt
     2581 ataatcccac atgcctatta tttaaacata tgtatactgt aataaattag tcccagaaga
     2641 gcatttcatc atactgtata attaatcaaa tttaaaaaac atttgtgttg aagagaagat
     2701 gactagattc acttgatttt ttttaatact gattggtcgt ttgaccagtc aggatccaga
     2761 ctgaaatatt ctcaaaaggt atgaactatt ttaatgaaat atttgagctg agaatttgct
     2821 tatgggacat tctatccttc taaagaaatc attagaaaat ggagaaaaag ttactagaaa
     2881 aagagaggga aatggggcga gagtatgaag aaagggtccc atgtgaatct aataattgaa
     2941 aagcaaatat gagatttatt ttagggttct tgttttagcg tccaaagcgg tcgcagttgc
     3001 cgttcagcga cttttctctg tttatgaaat aaaaacgctc cacttgttct tttcttcttt
     3061 caacccactt tgttataact attaacaaat attgcttggt ccaaatttta ttatactgaa
     3121 ttattttagt ttttttgtat catcatcctc aatgttagtt ttgaaatcct aggtcagtaa
     3181 gtcaataacg aaaatgacaa gtttaatttt gtaattggaa cttacatgtc acggtctttg
     3241 tttacgatca ttgccacgaa ttgtttagat acgggctttt tttttctttc cgtgttgatg
     3301 attatcgttt cttcttacat atcttgatca atcactaaca tttacataac ctacaatttt
     3361 gtaatataat tgcaatactc gagatcttca aatggtgata ctgttaataa aattacgaac
     3421 ttttaatcat catcaaaatc tatagaaaaa gatacatagg accaaaacaa gcctaaaaca
     3481 aagagttata atttacaaat aaatgcatgt attttgttac ttttgttaag gattttcata
     3541 tcaatgcatg cataatgcat tctcttttta ttttatttat aatgcattct tttataatat
     3601 tcggatccaa aattatgaat taattccaga ttgccataaa tgtctttctt tgtttatgct
     3661 gacttttttc ttttcttaac ttatatagta agtaaaccag aaaggtggaa gatacaaagc
     3721 aatttcattg cattaataaa cttcaaatgt attatccaat aatcaaaatt tatctagtaa
     3781 ttcacaaaaa aaaaaaatct gtctatcatt caaaatttca ccaatttgga taaatatcta
     3841 gaaaatttta aaatacaatt caataacact attaacatat caatcaatat ataacattgc
     3901 aaaagtttac ttgatcatta gtatattcat tttaaggtta aaagatcttt aagaaaagga
     3961 aattcaaatt atattgttgg aaaaaaatac aaatcaaaga cagcaaaaaa agtaacaaaa
     4021 aaaaaaggta gaagaaactg aaacgcggaa aggaggcaaa atcttctcgt cgtcgttgtc
     4081 gccgtcttca gagctacaaa cgaaaaaact cgcttccgtt tcgatttctc cattgttatt
     4141 gtttcttcag tgaagctttt ttcttcgaga aatttctaag atctaccaca tgctactaac
     4201 atgagaccga attctcgccc tatagtgagt cgtattacct ggccgtcgtt ttacaacgtc
     4261 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     4321 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     4381 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4441 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     4501 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     4561 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     4621 cgaaacgcgc ga
//
