LOCUS       sequence-396369-        5584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains SCOOP12 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP05
FEATURES             Location/Qualifiers
     source          1..5584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5170..5189)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5171..5189)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5199..5221)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5415..5434)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5534..5556
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc taataggttt cgagtactgt attgatgttt
     2281 aactgtattg tatatgtatt taattttcta ttccctgcca tcataaatca agctttggaa
     2341 aagtctcaaa catgtataat gtctttcgta cacaatgttt tacgttcttt tttttttgtt
     2401 ttcatttcaa tataatgata gaagtttttt ttttgtgtag cagagatatg taaagataat
     2461 cagattgaaa ctagctagag aaccaaaaat atttaaatgc aaacaaaatt aaagatttta
     2521 tatatgaatt acgtaagagg aaacaaaact aaactaaagt aggaagaaca tgttcactat
     2581 agagtaactc tttacaaact atttcttatt gataacaaac ttgatttatt ctcacaccac
     2641 cgcataaaaa acgagtattt ttaacaccga aaaccattat ctaacgatat gataatgttc
     2701 aaaaaaacct tttgacatcg ctaaatgctc gtaaagtagt cgttaaatat tctgttacga
     2761 ttatttgaca actaaatatc gtaaacgaaa atcgagagtc gctaaactta ttagacttat
     2821 tattaatcgt cttgtaacga ctaataataa atcagccact ttagataaag tttttttcat
     2881 actgctatcg tgtcaccaat ataatagcct ttttctatct gaaattatat attctacatt
     2941 agctatgtta cgtaatacat atttggaaac tatcgaaata tcttgttctt attaaaacct
     3001 catccacgat tatcttaacc ggagcactag ccataaccat attcatttcc tcatctttac
     3061 tccataatct aagtcacttt agcagtaaca taatagaaat gccttctatc tagtgatcgg
     3121 ttttgactat ctcaaaaata tttatatgca agagttattt aatattatat attcacattt
     3181 aaccagaaac aatctgggat tcagatatat gactttaaat atatgggttt aaataagatc
     3241 aggaattatg gattcaaata tttgacagct gttacaccat gaatgataag attggatcag
     3301 agctagtatc taaaatctag aactattttt cattaattac tactttgtag agaagaatag
     3361 tcaatgagaa acaaaagggt taatattttc atacgtttat catctgtttt aataagaaca
     3421 agagcgtcaa cttagttccg gactcttgat cctagcttaa agagtttatt attattatag
     3481 ttccacattc aaccaaaaac actctcggat tcagatagga acaagagggt caacttctta
     3541 aagattaatg actagtggag aaattacgaa gtttgccttg tatttttctt tagtatttgt
     3601 ttactctcta ccgccctacc ggcgatgcct caccattcgg gtacataaat gaaattctga
     3661 tatcaagaaa aattggaatc tattttcgaa aagtcatact agcgttttct acctttttag
     3721 ttacttacat tttttttttt cgtacctttt tagttactta cataaaattc cgttttagat
     3781 gtcttttctt ttttaataaa gaaatttctt gtatcgtctc ttttctgtgt ttcagtaatt
     3841 tatgtacata tacacatgtg ccaagtatat cttatccaga taagcttcag attttgtaag
     3901 actggtgact ttaaatttta aaagaaaaca aaaaagtctg ttgacttcct tattgtaata
     3961 gaggtcaaag tctcaagttt gaccattaat gtaaataatt atccatttgg aaatatctat
     4021 tttagtccaa tagaaaattg attcagtaaa tgattctgat aaaatgcccg tatttccatg
     4081 aggcacttaa tgattaaatt ctcatagtgt ttgaactcca acttcaacat aaacaaagag
     4141 aaatcttcaa tggattttgc agctgcatga ttaagaaatt ttgaataagc attttgcaag
     4201 aagtagtccc tcttgaaatt tatgaaatgc tatgtacaac cgcatttcta atctatagca
     4261 aagccggcca aaactatata ccatcaacca catctctctg tttctcgatc cattcgtttg
     4321 gcaaaagctg ctggattcct tttgttttcg ctctattaca gttattcctc tagtcttttg
     4381 ttacatcaaa ctctaagctt tcacccaaat gaatttggtc ttgattttcc tctggattgg
     4441 tatttgtttg gcaataaagt ttcaactcaa accaaaatac ttatcaaaat atcttgcaat
     4501 gtcaaagata tttgataagc ttcataacta tcttaaccgg atcacgtggc ataaccatct
     4561 tcggttcctc aactttaccc tttcataatc tttgtcactt aagtagtaac ataacagcgt
     4621 tcttttgaat gctttctata tagtgatgtt gttcattatt ccctatccca aaaaaaaact
     4681 tatatgcaag gacttattta atgttatatt tacatttgtt tagctatagt atatttttac
     4741 attcaaccaa aataaaaatt cggatcctga tatatgaact tcaaagatag tggcttaaga
     4801 tcacgaacct cagattcaaa tttgtaagga gctatgaaaa tattaacctt agatcggatc
     4861 tgaaaatcct gaactatttt atgtcaccat acttagtaag aaactaatgg tcaattaaca
     4921 acaaactaaa aggattaata tttgtatacg taattctttg tttttataag acaagagggt
     4981 caacttgtta aagggtaatg atcagtgagt attattgact agtgaagaaa actaaaaagt
     5041 caacttggta ttattaggtt ttggtttgtt tcattttttc gtggaacgtt ccctccacct
     5101 gcatgtacat aaatatgagt ctaaactcga gaaaaaaata aagatcgaga acatgagacc
     5161 gaattctcgc cctatagtga gtcgtattac ctggccgtcg ttttacaacg tcgtgactgg
     5221 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5281 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5341 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     5401 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     5461 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     5521 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5581 gcga
//
