LOCUS       sequence-396373-        6783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains WRKY70 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Calabria J, Wang L, Rast-Somssich MI, Chen HW, Watt M, Persson S, 
            Andersen TG, Idnurm A, Somssich M
  TITLE     Resolving spatially distinct phytohormone response zones in 
            Arabidopsis thaliana roots colonized by Fusarium oxysporum.
  JOURNAL   J Exp Bot. 2025 Jan 17:erae516. doi: 10.1093/jxb/erae516.
  PUBMED    39823275
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Jan 17:erae516. doi: 10.1093/jxb/erae516."
COMMENT     Sequence Label: pGG-PIP44
FEATURES             Location/Qualifiers
     source          1..6783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6369..6388)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6370..6388)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6398..6420)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6614..6633)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6733..6755
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tcattgtaga tatgatatat gaagcttccc
     2281 cattatttcc ttgtttattt ccccttttga tcgcacaaaa aatatttgtg tgaaaacaaa
     2341 agtaacgttt tagggttgta caattattgt tggtaccagc atacgatacc cactcactcc
     2401 ttttgcaata attcttttta cgcatcacaa tcaactgtac atatcttgat tcatgaacct
     2461 ttctagaaaa aaacgaaagt tgaaatgtat atattatttt agtcactcga atcgcattaa
     2521 tccattattc aacctttatt cattaccatt gcagcggttt aatcatttat tcttgcgcca
     2581 aagatcttcc tatttccaaa ggaataaacg caattgaaat tcttggggag ttatacattt
     2641 gtgtattgat aagacctacc gcacttatgc agcttctacg atttcgaacg caccgttttg
     2701 accgcttccc cgactcgagt ttttttttta cattaactat aacagttgtt ttagagtata
     2761 gtcaatcgat tcgaataaat atttcagaac cctataattc ttatgttgta tatactaata
     2821 catatagata tgaattaata atgacaaatt tctaataagt agtacaaccc caacatgcaa
     2881 aaccctatgt tgtcttgtag tgatatattg atcaattagc tgtctatagc cccccttatt
     2941 aaatggtctt ttggagacat atatagacaa aatagttgcc tacacacaac ttgatgactt
     3001 ttattgttat cttcattgta cttcgatcca tttgtgcatt acataaaaaa aaattgcaaa
     3061 attcacgttg atcattgata ctaattattt gggttgatat aggctaagca gtagtggaat
     3121 ggaaacacaa atagacattc atgagattag ggaacagcgc aatataatac tcctagtgac
     3181 aaactgataa tgctaaaaca gtagctttaa tctaactaat gggttcaata gttcaattaa
     3241 tgaagggccg ttcaatgctt ttaaacggac tgcggagtat taaataaata tctctaatgg
     3301 gaaataaaat tgttcaaatc ttaatccact aatgctcgtt tgttttgtgc aattagattc
     3361 aagtccacaa ccaataaaat ttaaaacatt tagcgagaag ttatcatgtg aggtgagaca
     3421 cataaatatg gaacggtgtt ggatgacaat ttcttgatta ttttatttta aataaagtta
     3481 ttaccttact gttggccatt ttgtagaaaa ttcaaaacta ttttaaaaat aattgttgga
     3541 tatatttttt ttttttatca gaaactaaaa tacaaaccat tcaacaaaaa ctacagccat
     3601 tcgtatttgg atacatgaat ccgttcacaa ttatatttca tgaatcatga taggtctgtg
     3661 tgatagagat atatgcgttg ttctttctct atgtattatg gctaacttta aataattaaa
     3721 tcgagattta gtcatagttt ggcaatgcaa tcacggataa agttcaaaag tttacaaatg
     3781 gaatttgaaa atcacatttt tgtcccaaaa tatgtatatt ctatatttat ttatttaaaa
     3841 gatttattat agtaaataaa taatgcaaaa tggatatact tttaaaaagt agagatagag
     3901 ttagcaattc gttaatggag gaactcaatt aactatattt aaaaagacag ctaagcttca
     3961 gatttgataa aacaaaagct tgattatagt atatttttaa ttattctttt tattgtttat
     4021 tcccttgtca actcctcttc cccattgaca gtttctcaca tcatccctac atagaaactg
     4081 tatataacag aaattaatta ttcctgtgac tctaataacc aattgttatc atatggttat
     4141 tggatccttt ctattggtcg gctacgtgag aaacaatgga ttagcagtaa aaagactaac
     4201 aaatacaaaa aaaatgccgg cctaataaat gtgattacaa tatacacaac tattcttagt
     4261 agaatatact aacttactac gctacagaac caactggttt atcatagtat ctcgcatatt
     4321 aacttaggct agagagccaa cctttttgaa ttaatgaaaa agttgagaca caaccactaa
     4381 actttacggc attaataacg gatctcattt atgaacctgc gtacaaattt ggtctgaagt
     4441 taagtggtac tatcccggaa ataacttaaa tcttatccta aactatattc tttccatttt
     4501 atagtacgag atgttttgga aaaaaaaaca aatagtttca taataaagta atattttcaa
     4561 gttcttatgc aatattttat gattagttat tagttatatt atgcattttc tttatgtttg
     4621 gttgaacttt ttaaaaatat gtaattttta atatacgtgt ttttagttaa acatcttata
     4681 ttttaaaacg gattaagtat ctttttagtc ataatttatc tttatacaca aaaaagtaat
     4741 actttgtcga ttcttaaccc atgcaattgt ttatgtgttc tgaaattgat tgatatgatc
     4801 ctagctcaac tggcgttaat agacttcgta tgaagggaaa aaatttcaaa atcattaatt
     4861 taaaagttcg acgtgaaaaa atgtaataat ttaacattct cttataatgc ttccgtaaga
     4921 taaatttatg gactattatc cgactttttg ataatgggta tgcaaaaact tgtcatatct
     4981 aaaaaaaata tcaccaatgt taatttatac ttgggcataa atgtatcatt aaaaaaacct
     5041 tggtggcttg gtgcatatag aagagagtga ggctgtgagg gagcactata gaccaactag
     5101 acatctctaa gcgaggagaa cccatccgca tcgcatgtat gcattgagat agctagggcc
     5161 taggggccat tgagtcgatc gaccacatac acctctttaa ataatcttct aatattaaag
     5221 ttttaaatca atgccccgcc caactctacc ttttccacga aaacggaata aagtgtttcc
     5281 gggtgaaaga aaatactttt caacgataga ttgtgttgct atttttatag aatatgttaa
     5341 atttaagtat aatataagat aggttcctat aaaaaaattg ggtagaaaag tttagctttg
     5401 atactccata tagtttatat attagtgcag cgaggtgata cgtttactag tctaacttaa
     5461 caaaaaaaaa agagttaatt agtttatata ttgattcttt caacgtaatc aatctgattt
     5521 agataatccg tcccaactcc caattatatc tgtcattcaa gtatttgatt acaagggcaa
     5581 ttcatttcgg actagttgat tgtaaatttc gtgtctcgtt atctatttga aatagtaggt
     5641 ctatttaggg ggtatacatc tggattgata gctgataagg actatataca tgtctttttg
     5701 aatttttgat atataaacta ctattcaaaa ttatcttcat agaaaaacgg atcatatcat
     5761 ttggatatca aatttaactc gagatttcaa ataaataatt cactattcca actgatctta
     5821 ctacatcagt aaaaaaaaat tggtagtaca atctcgagga acaaaaaagt ttacaaatga
     5881 aatttagaat cttgtttaat ctttaaatgg atagtttttc ataactcttt taattagtct
     5941 agtttttgat acaaatttta tccggttata gaaaacatta aaggacccta agtttggatt
     6001 tcagcaaaat tatatatatt ccattctcac ttagggatga aatgacatca ttcatcacta
     6061 cgtcatttga gcttacgtca agctcaccat aagcaaaaga aatgggtgga taagttcaag
     6121 ttccatctca tttcctcaca catctctctt tcttccttct catttcctcg catcttcctc
     6181 taaaaaaaaa aatcaaagaa accaacaaaa taaaaagtgc ccatcattac aaatcttatt
     6241 tccaccttat atttacacac acttctcttc tttcctttaa tcatacaaac cacaccaaga
     6301 ggaaagaaaa ataagatacc actcaccaaa aacttcctca aaactaacaa catgagaccg
     6361 aattctcgcc ctatagtgag tcgtattacc tggccgtcgt tttacaacgt cgtgactggg
     6421 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     6481 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     6541 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     6601 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     6661 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     6721 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     6781 cga
//
