LOCUS       sequence-396435-        1354 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Dendra2-NS2B in Mammalian / Mouse Cell Lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Aravinth S, Varghese JM, Pant N, Mondal PP
  TITLE     Super-resolution optical microscopy reveals accumulation of 
            photoactivable dengue protein (Dendra2-NS2B) in the endoplasmic 
            reticulum.
  JOURNAL   Sci Rep. 2025 Mar 26;15(1):10358. doi: 10.1038/s41598-025-94135-6.
  PUBMED    40133359
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2025-03-26"; volume: "15(1):10358. doi"; pages: " 
            10.1038/s41598-025-94135-6"
COMMENT     Sequence Label: Dendra2-NS2B
FEATURES             Location/Qualifiers
     source          1..1354
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..693
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 accatgaaca ccccgggaat taacctgatc aaggaggaca tgcgcgtgaa ggtgcacatg
       61 gagggcaacg tgaacggcca cgccttcgtg atcgagggcg agggcaaggg caagccctac
      121 gagggcaccc agaccgccaa cctgaccgtg aaggagggcg cccccctgcc cttcagctac
      181 gacatcctga ccaccgccgt gcactacggc aaccgggtgt tcaccaagta ccccgaggac
      241 atccccgact acttcaagca gagcttcccc gagggctaca gctgggagcg caccatgacc
      301 ttcgaggaca agggcatctg caccatccgc agcgacatca gcctggaggg cgactgcttc
      361 ttccagaacg tgcgcttcaa gggcaccaac ttccccccca acggccccgt gatgcagaag
      421 aagaccctga agtgggagcc cagcaccgag aagctgcacg tgcgcgacgg cctgctggtg
      481 ggcaacatca acatggccct gctgctggag ggcggcggcc actacctgtg cgacttcaag
      541 accacctaca aggccaagaa ggtggtgcag ctgcccgacg cccacttcgt ggaccaccgc
      601 atcgagatcc tgggcaacga cagcgactac aacaaggtga agctgtacga gcacgccgtg
      661 gcccgctaca gccccctgcc cagccaggtg tggtccggcg acagcggcgt gtacaagact
      721 cgagtgcgtg agtgcatctc catccacgtt ggccaggctg gtgtccagat tggcaatgcc
      781 tgctgggagc tcaccatggc aagctggcca ttaaatgagg ctatcatggc agtcgggatg
      841 gtgagcattt tggccagttc actcctaaag aatgacattc ccatgacagg accattagtg
      901 gctggagggc tcctcactgt gtgctacgtg ctcactggac gatcggccga tttggaactg
      961 gagagagccg ccgatgtcaa atgggaagat caggcagaga tatcaggaag cagtccaatc
     1021 ctgtcaataa caatatcaga agatggtagc atgtcgataa aaaacgaaga ggaagaacaa
     1081 acactgacca tactcattag aacaggattg ctggtgatct caggactttt tcctgtatca
     1141 ataccaatca cggcagcagc atggtacctg tgggaagtga agaaacaacg gatccaccgg
     1201 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     1261 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     1321 cttgtttatt gcagcttata atggttacaa ataa
//
