LOCUS       sequence-396452-        7087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses P1 S' tail fibre and U' chaperone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Huan YW, Fa-Arun J, Wang B
  TITLE     The Role of O-antigen in P1 Transduction of Shigella flexneri and 
            Escherichia coli with its Alternative S' Tail Fibre.
  JOURNAL   J Mol Biol. 2022 Sep 15;434(21):167829. doi: 
            10.1016/j.jmb.2022.167829.
  PUBMED    36116540
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2022-09-15"; volume: "434(21):167829. doi"; pages: " 
            10.1016/j.jmb.2022.167829"
COMMENT     Sequence Label: 4A3.S'U'
FEATURES             Location/Qualifiers
     source          1..7087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(20..63)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    66..87
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             3675..3692
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3745..3763)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3759..3806
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    3838..3858
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3859..3916
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(3940..3957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(4219..5169)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5217..5439
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5931..6025)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(6055..6846,6847..6915))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIQYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6678..6697
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6916..7020)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(7085..7087,1..16)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
       61 ttctggaatt cgcggccgct tctagagatc accccgtccg cagggcgggg tgacaagtta
      121 cttatcttac aatgaggctt cacaacattg attagggaaa atcatgaatg acgttacagt
      181 tgttacatcg gttacttacc catcatccga gtcgttggct ctggtggccg atgtgcaata
      241 ccacgaacca tatctgtcag ccgcgctaaa ccgaaaattc agggggattg ttgacccggg
      301 attttatgcc ggtttcttac ctaagcctgg cggtgggatg aacctgttaa ttacctcagt
      361 ggatggtgat aaaaccgcag gcgcggcgtc ggtggatatt ggtgaatttt accaggtaac
      421 tattcagcaa cgtaaggata tttctcttgc acttagtgca ggcaagaaat atgcaattgt
      481 gctgaaggga agatacctcc ttggagaaga tacctaccag gtgaataccg cgtcacatat
      541 tcatgcggct gaatttgttg ccagaaccta taccgattca tatcagttag gagatgggga
      601 gctgcttgtt tgtacggtga atatccctgc tagtgtatct gccattaccc aggagatgat
      661 tgatacatcc gagcgtatca accgctcgat cggcattgat atttcagact ctgtaaccag
      721 taccagaagt gatgttgctg caagttcgct ggcagttaaa aaagcctacg atctggcgaa
      781 aagcaagtat acggcacagg atgcaagcac aacgcaaaag ggattagttc agctcagtag
      841 cgcaactaac agcgacagcg aaacaatggc ggctacccct aaagctgtta agtctataaa
      901 agatctggct gataccaaag cgccaataga aagcccgagt ctgacaggaa cgccaaccgc
      961 gccgacggca gcgcaaggta caaacagcac gcagatcgca aatacagcct ttgttaaggc
     1021 agctataact gcacttatca acggtgcgcc tggcacactg gatacgctga aagaaatagc
     1081 ggctgcgatc aataacgacc cgaattacag cacaactatc aacaatgcct tggctctcaa
     1141 agcgcctttg gcaagccctg cattaacggg tgtccctact gcgcctacgg ctgcacaggg
     1201 cacaaacaat acgcagatcg ctacgactgc ttacgtacgg gctgctatct ctgcattggt
     1261 cggctcatca cctgaagctc ttgataccct gtatgagctt gcagcagcac tgggcaatga
     1321 cccgaacttt gcgacaacaa tgacaaatgc gctggcaggg aaacagccac ttgatgcaac
     1381 tttaaccgcg cttgctggtc ttgcgacagg cgcaaataaa ttgccgtact ttaccggtac
     1441 agacactgtt tctcagactg acttaacgtc agttggtcgc gatattctgg ccaaaacaag
     1501 cattcttgct gttatccaat accttggttt aagagaactc ggtaccagcg gtgaaaagat
     1561 ccccctgttg agcacggcta acacatggag tgcacgccag actttcaacg gcgggatcac
     1621 cggggcgctg acagggaacg ccgataccgc aacgaaattg aaaacagcca gaaacattaa
     1681 tggcgtcagg ttcgatggtt ctggtgacat taatatcaat actctggtat cgcgcggtcg
     1741 cgtaacggcc ctggaggcga atgcacaggg aacatccggg attcagctgt atgaggcata
     1801 caacaatggc tacccttccc cctatggcaa tgtgcttcac cttaaaggtg ccaccgctgc
     1861 tggcgaaggt gagttattca ttggctggag tggcacgagc ggtgcccatg cgcccgtaca
     1921 tatccgttcg cggcgggata ctgattctgc caactggtct gaatgggcgc aggtctatac
     1981 gtcaaaagat tcaattcccg gcgtcaatgc caaaggggat caggatacct ctggtaatgc
     2041 ggctacagcg accaagttgc agacagcatg tactatcaac ggtgtctcgt ttgatggttc
     2101 taaaaatatt gagctaacgg ctgaaaattt aaatcttgag cgaacagtag aattagccgc
     2161 tgggtcattg cagaaaaatc agaacggcgc ggatattcct ggaaaagata ccttcacaaa
     2221 aaatattggt gcatgtcgcg cttttcacag ttctattagt acaggtgcag ggaactggac
     2281 aacggcacaa ttgattgaat ggctggattc tcaaggggca ttcaatcacc catactggat
     2341 gtgcaaatgt tcatggtcgt acggcaataa taaaattata accgatactg gctgtggaac
     2401 tattcatctt gcaggttgcg ttattgaggt tatgggtaat aaaggtgcca tgaccatccg
     2461 tgtaacaaca ccaagcactt ccagcggtgg cggaatcact aacgctcaat tcacttatat
     2521 taatcatggt gatgcttacg ctcctggctg gcgacgagac tacaacacga aaaacctgca
     2581 acctgcattt gctttagggc agacaggaaa cagggttgca aatgataaag ctgttggctg
     2641 gaactggaat agcggtgttt atgatgcaga cctaaaaggc gcatcaacat taattcttca
     2701 tttcaatatg aacgcgggta gctgcccggc tgtacaatta cgcgtgaatt ataagaacgg
     2761 cggtatttat tatcgttcag cgcgtgatgg ttatggattt gaggctgact ggtcagagtt
     2821 ttacaccaca acccgcaaac cctctgcggg ggatgttggt gcatatacgc aggcagaatg
     2881 taactcaagg tttattacag gtattcgcct gggcggtctg tcatctgtcc agacatggaa
     2941 tggccccggc tggtctgaca ggtcaggtta tgtcgttacg ggttcagtta acgggaaccg
     3001 tgatgaatta attgatacaa cacaggcaag gccaattcag tattgcatta atgggacgtg
     3061 gtataacgcg gggagtattt aattatgatg cacttaagaa atattacagc tggcaaccct
     3121 aaaacaaaag agcaatacca gctaacgaaa caatttaaca tcaaatggct ttatacagag
     3181 gatggaaaaa actggtatga ggaacaaaag aatttccagt atgatacgtt gaaaatggcc
     3241 tatgaccaca acggcgttat tatttgtatt gaaaaggatg tttcagcaat taatccagaa
     3301 ggcgcaagcg tcgttgaatt acctgatatt acagcaaatc gccgggctga tatttctggt
     3361 aaatggatgt tcaaagatgg cgtagtggta aagcgaactt ataccgagga agagcagagg
     3421 caacaagcgg aaaatgaaaa gcaaagtctg ctacagctcg tcagggataa aacccagcta
     3481 tgggactcac agctacggct gggtatcatt tccgccgaga ataagcagaa attaaccgag
     3541 tggatgctct ttgcgcagaa agtcgaatcc acagacacct ccagcctacc agtaacattt
     3601 cccgaacaac ctgaatgaga caaggcaagg atccgaattc gagctccgtc gacaagcttg
     3661 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
     3721 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     3781 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttaataatac
     3841 tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     3901 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     3961 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tctcgaggtt
     4021 acattgtcga tctgttcatg gtgaacagct ttgaatgcac caaaaactcg taaaagctct
     4081 gatgtatcta tcttttttac accgttttca tctgtgcata tggacagttt tccctttgat
     4141 atgtaacggt gaacagttgt tctacttttg tttgttagtc ttgatgcttc actgatagat
     4201 acaagagcca taagaacctc agatccttcc gtatttagcc agtatgttct ctagtgtggt
     4261 tcgttgtttt tgcgtgagcc atgagaacga accattgaga tcatacttac tttgcatgtc
     4321 actcaaaaat tttgcctcaa aactggtgag ctgaattttt gcagttaaag catcgtgtag
     4381 tgtttttctt agtccgttat gtaggtagga atctgatgta atggttgttg gtattttgtc
     4441 accattcatt tttatctggt tgttctcaag ttcggttacg agatccattt gtctatctag
     4501 ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca ccaatttcat
     4561 attgctgtaa gtgtttaaat ctttacttat tggtttcaaa acccattggt taagcctttt
     4621 aaactcatgg tagttatttt caagcattaa catgaactta aattcatcaa ggctaatctc
     4681 tatatttgcc ttgtgagttt tcttttgtgt tagttctttt aataaccact cataaatcct
     4741 catagagtat ttgttttcaa aagacttaac atgttccaga ttatatttta tgaatttttt
     4801 taactggaaa agataaggca atatctcttc actaaaaact aattctaatt tttcgcttga
     4861 gaacttggca tagtttgtcc actggaaaat ctcaaagcct ttaaccaaag gattcctgat
     4921 ttccacagtt ctcgtcatca gctctctggt tgctttagct aatacaccat aagcattttc
     4981 cctactgatg ttcatcatct gagcgtattg gttataagtg aacgataccg tccgttcttt
     5041 ccttgtaggg ttttcaatcg tggggttgag tagtgccaca cagcataaaa ttagcttggt
     5101 ttcatgctcc gttaagtcat agcgactaat cgctagttca tttgctttga aaacaactaa
     5161 ttcagacata catctcaatt ggtctaggtg attttaatca ctataccaat tgagatgggc
     5221 tagtcaatga taattacatg tccttttcct ttgagttgtg ggtatctgta aattctgcta
     5281 gacctttgct ggaaaacttg taaattctgc tagaccctct gtaaattccg ctagaccttt
     5341 gtgtgttttt tttgtttata ttcaagtggt tataatttat agaataaaga aagaataaaa
     5401 aaagataaaa agaatagatc ccagccctgt gtataactca ctactttagt cagttccgca
     5461 gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta caaaacagac cttaaaaccc
     5521 taaaggctta agtagcaccc tcgcaagctc gggcaaatcg ctgaatattc cttttgtctc
     5581 cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct gcgctcacgg
     5641 ctctggcagt gaatgggggt aaatggcact acaggcgcct tttatggatt catgcaagga
     5701 aactacccat aatacaagaa aagcccgtca cgggcttctc agggcgtttt atggcgggtc
     5761 tgctatgtgg tgctatctga ctttttgctg ttcagcagtt cctgccctct gattttccag
     5821 tctgaccact tcggattatc ccgtgacagg tcattcagac tggctaatgc acccagtaag
     5881 gcagcggtat catcaacagg cttacccgtc ttactgtccc tagtgcttgg attctcacca
     5941 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     6001 attactggat ctatcaacag gagtccaagc gagctcgtaa acttggtctg acagttacca
     6061 atgcttaatc agtgaggcac ctatctcagc tatctgtcta tttcgttcat ccatagttgc
     6121 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6181 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6241 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6301 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6361 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6421 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6481 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6541 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6601 tggtgagtac tcaaccaagt cattctgaga atattgtatg cggcgaccga gttgctcttg
     6661 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6721 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6781 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6841 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6901 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6961 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7021 cacatttccc atggtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     7081 taaaaat
//
