LOCUS       sequence-396459-        3783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E-cadherin with a LOV2 insert after T133 for blue light 
            inhibition in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Nzigou Mombo B, Bijonowski BM, Raab CA, Niland S, Brockhaus K, 
            Muller M, Eble JA, Wegner SV
  TITLE     Reversible photoregulation of cell-cell adhesions with 
            opto-E-cadherin.
  JOURNAL   Nat Commun. 2023 Oct 9;14(1):6292. doi: 10.1038/s41467-023-41932-0.
  PUBMED    37813868
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-10-9"; volume: "14(1):6292. doi"; pages: " 
            10.1038/s41467-023-41932-0"
COMMENT     Sequence Label: opto-E-cad GFP
FEATURES             Location/Qualifiers
     source          1..3783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3070..3582
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIE"
     primer_bind     complement(3109..3130)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3370..3389)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3717..3738
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgggccctt ggagccgcag cctctcggcg ctgctgctgc tgctgcaggt ctcctcttgg
       61 ctctgccagg agccggagcc ctgccaccct ggctttgacg ccgagagcta cacgttcacg
      121 gtgccccggc gccacctgga gagaggccgc gtcctgggca gagtgaattt tgaagattgc
      181 accggtcgac aaaggacagc ctatttttcc ctcgacaccc gattcaaagt gggcacagat
      241 ggtgtgatta cagtcaaaag gcctctacgg tttcataacc cacagatcca tttcttggtc
      301 tacgcctggg actccaccta cagaaagttt tccaccaaag tcacgctgaa tacagtgggg
      361 caccaccacc gccccccgcc ccatcaggcc tccgtttctg gaatccaagc agaattgctc
      421 acatttccca actcctctcc tggcctcaga agacagaaga gagactgggt tattcctccc
      481 atcagctgcc cagaaaatga aaaaggccca tttcctaaaa acctggttca gatcaaatcc
      541 aacaaagaca aagaaggcaa ggttttctac agcatcactg gccaaggagc tgacacaccc
      601 cctgttggtg tctttattat tgaaagagaa acaggatggc tgaaggtgac agagcctctg
      661 gatagagaac gcattgccac atacactctc ttctctcacg ctgtgtcatc caacgggaat
      721 gcagttgagg atccaatgga gattttgatc acggtaaccg atcagaatga caacaagccc
      781 gaattcaccc aggaggtctt taaggggtct gtcatggaag gtgctcttcc aggaacctct
      841 gtgatggagg tcacagccac actggcgacc accctggaac gtattgaaaa gaactttgtg
      901 attaccgatc cgcgtctgcc ggataacccg attatttttg cgagcgatag ctttctgcag
      961 ctgaccgaat atagccgtga agaaattctg ggccgtaact gccgttttct gcagggcccg
     1021 gaaaccgatc gtgcgaccgt gcgtaaaatt cgtgatgcga ttgataacca gaccgaagtg
     1081 accgtgcagc tgattaacta taccaaaagc ggcaaaaaat tttggaacct gtttcatctg
     1141 cagccgatgc gtgatcagaa aggcgatgtg cagtatttta ttggcgtgca gctggatggt
     1201 accgaacatg tgcgtgatgc ggcggaacgt gaaggcgtga tgctgatcaa gaaaaccgcg
     1261 gaaaacattg atgaagcgga cgcggacgat gatgtgaaca cctacaatgc cgccatcgct
     1321 tacaccatcc tcagccaaga tcctgagctc cctgacaaaa atatgttcac cattaacagg
     1381 aacacaggag tcatcagtgt ggtcaccact gggctggacc gagagagttt ccctacgtat
     1441 accctggtgg ttcaagctgc tgaccttcaa ggtgaggggt taagcacaac agcaacagct
     1501 gtgatcacag tcactgacac caacgataat cctccgatct tcaatcccac cacgtacaag
     1561 ggtcaggtgc ctgagaacga ggctaacgtc gtaatcacca cactgaaagt gactgatgct
     1621 gatgccccca ataccccagc gtgggaggct gtatacacca tattgaatga tgatggtgga
     1681 caatttgtcg tcaccacaaa tccagtgaac aacgatggca ttttgaaaac agcaaagggc
     1741 ttggattttg aggccaagca gcagtacatt ctacacgtag cagtgacgaa tgtggtacct
     1801 tttgaggtct ctctcaccac ctccacagcc accgtcaccg tggatgtgct ggatgtgaat
     1861 gaagccccca tctttgtgcc tcctgaaaag agagtggaag tgtccgagga ctttggcgtg
     1921 ggccaggaaa tcacatccta cactgcccag gagccagaca catttatgga acagaaaata
     1981 acatatcgga tttggagaga cactgccaac tggctggaga ttaatccgga cactggtgcc
     2041 atttccactc gggctgagct ggacagggag gattttgagc acgtgaagaa cagcacgtac
     2101 acagccctaa tcatagctac agacaatggt tctccagttg ctactggaac agggacactt
     2161 ctgctgatcc tgtctgatgt gaatgacaac gcccccatac cagaacctcg aactatattc
     2221 ttctgtgaga ggaatccaaa gcctcaggtc ataaacatca ttgatgcaga ccttcctccc
     2281 aatacatctc ccttcacagc agaactaaca cacggggcga gtgccaactg gaccattcag
     2341 tacaacgacc caacccaaga atctatcatt ttgaagccaa agatggcctt agaggtgggt
     2401 gactacaaaa tcaatctcaa gctcatggat aaccagaata aagaccaagt gaccacctta
     2461 gaggtcagcg tgtgtgactg tgaaggggcc gccggcgtct gtaggaaggc acagcctgtc
     2521 gaagcaggat tgcaaattcc tgccattctg gggattcttg gaggaattct tgctttgcta
     2581 attctgattc tgctgctctt gctgtttctt cggaggagag cggtggtcaa agagccctta
     2641 ctgcccccag aggatgacac ccgggacaac gtttattact atgatgaaga aggaggcgga
     2701 gaagaggacc aggactttga cttgagccag ctgcacaggg gcctggacgc tcggcctgaa
     2761 gtgactcgta acgacgttgc accaaccctc atgagtgtcc cccggtatct tccccgccct
     2821 gccaatcccg atgaaattgg aaattttatt gatgaaaatc tgaaagcggc tgatactgac
     2881 cccacagccc cgccttatga ttctctgctc gtgtttgact atgaaggaag cggttccgaa
     2941 gctgctagtc tgagctccct gaactcctca gagtcagaca aagaccagga ctatgactac
     3001 ttgaacgaat ggggcaatcg cttcaagaag ctggctgaca tgtacggagg cggcgaggac
     3061 gacccgcgga gcaaggggga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3121 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     3181 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3241 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     3301 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3361 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3421 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3481 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     3541 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     3601 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     3661 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     3721 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgatcaag
     3781 taa
//
