LOCUS       sequence-396462-       10036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Landing Pad Utopia_v7 donor plasmid for AAVS1 site-specific 
            integration in human cells. Use NeoR and eGFP as positive selection 
            marker for AAVS1-targeted insertion and RFP as negative selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Wan Y, Cohen J, Szenk M, Farquhar KS, Coraci D, Krzyszton R, Azukas 
            J, Van Nest N, Smashnov A, Chern YJ, De Martino D, Nguyen LC, Bien 
            H, Bravo-Cordero JJ, Chan CH, Rosner MR, Balazsi G
  TITLE     Nonmonotone invasion landscape by noise-aware control of metastasis 
            activator levels.
  JOURNAL   Nat Chem Biol. 2023 Jul;19(7):887-899. doi: 
            10.1038/s41589-023-01344-z. Epub 2023 May 25.
  PUBMED    37231268
REFERENCE   2  (bases 1 to 10036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-023-01344-z"; journalName: "Nat Chem Biol"; date: 
            "2023-07"; volume: "19"; issue: "7"; pages: "887-899"
COMMENT     Sequence Label: LPUtopia-7
FEATURES             Location/Qualifiers
     source          1..10036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    358..1161
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    1204..1237
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(1258..1291)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1576..2379
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1639..1658)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2249..2268
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2386..2434
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(2445..2478)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2547..2566)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2738..3117
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3118..3321
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3366..3385
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3366..3384
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3423..3432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3429..3431,3432..3434,3435..4148)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3429..3431,3432..3434,3435..4145)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3735..3754)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4082..4103
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4176..4194)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4177..4194)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4214..4231)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4220..4444
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4454..4501
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    4891..5727
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     repeat_region   5737..5771
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        5998..6201
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6198..6222
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    6991..7215
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(7376..7964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7456..7475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8135..8926,8927..8995))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8758..8777
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8996..9100)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9126..9581)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9263..9284)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9475..9494
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9708..9730
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9722..9739
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9723..9739
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   9869..9931
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tttatgttta tttatttatt aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca
       61 aaacttttat gagggacagc cccccccaaa gcccccaggg atgtaattac gtccctcccc
      121 cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat
      181 ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt
      241 tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa
      301 aggcctccac ggccactagt aacggccgcc agtgtgctgg aattcgccct tggtacctgc
      361 tttctctgac cagcattctc tcccctgggc ctgtgccgct ttctgtctgc agcttgtggc
      421 ctgggtcacc tctacggctg gcccagatcc ttccctgccg cctccttcag gttccgtctt
      481 cctccactcc ctcttcccct tgctctctgc tgtgttgctg cccaaggatg ctctttccgg
      541 agcacttcct tctcggcgct gcaccacgtg atgtcctctg agcggatcct ccccgtgtct
      601 gggtcctctc cgggcatctc tcctccctca cccaacccca tgccgtcttc actcgctggg
      661 ttcccttttc cttctccttc tggggcctgt gccatctctc gtttcttagg atggccttct
      721 ccgacggatg tctcccttgc gtcccgcctc cccttcttgt aggcctgcat catcaccgtt
      781 tttctggaca accccaaagt accccgtctc cctggcttta gccacctctc catcctcttg
      841 ctttctttgc ctggacaccc cgttctcctg tggattcggg tcacctctca ctcctttcat
      901 ttgggcagct cccctacccc ccttacctct ctagtctgtg ctagctcttc cagccccctg
      961 tcatggcatc ttccaggggt ccgagagctc agctagtctt cttcctccaa cccgggcccc
     1021 tatgtccact tcaggacagc atgtttgctg cctccaggga tcctgtgtcc ccgagctggg
     1081 accaccttat attcccaggg ccggttaatg tggctctggt tctgggtact tttatctgtc
     1141 ccctccaccc cacagtgggg cacgcgtaag ggcgaattct gcagatatcg aagttcctat
     1201 tccgaagttc ctattcttca aatagtatag gaacttcgtc gagatcgatg gcgcgccata
     1261 acttcgtata gcatacatta tacgaagtta tgaatttcga gcagtgtggt tttcaagagg
     1321 aagcaaaaag cctctccacc caggcctgga atgtttccac ccaatgtcga gcagtgtggt
     1381 tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg aatgtttcca cccaatgtcg
     1441 agcaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc
     1501 ggcgcggtcc caggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc
     1561 gaccctgcag ccaatatggg atcggccatt gaacaagatg gattgcacgc aggttctccg
     1621 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     1681 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     1741 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     1801 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     1861 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     1921 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     1981 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     2041 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     2101 aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc
     2161 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     2221 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     2281 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     2341 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag gggatcggca ataaaaagac
     2401 agaataaaac gcacgggtgt tgggtcgttt gttcggatca attcataact tcgtatagca
     2461 tacattatac gaagttatgg cgcgccttaa ttaaggatcc gtcgacggat cgggagatct
     2521 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2581 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     2641 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     2701 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
     2761 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     2821 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2881 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2941 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     3001 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     3061 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     3121 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     3181 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     3241 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     3301 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
     3361 gaaattaata cgactcacta tagggagacc caagcttggt accgagctcg gatccaccgg
     3421 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3481 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     3541 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     3601 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     3661 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     3721 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     3781 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     3841 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     3901 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     3961 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     4021 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     4081 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     4141 acaagtaaag cggccgcgac tctagagggc cctattctat agtgtcacct aaatgctaga
     4201 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     4261 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4321 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     4381 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     4441 atggcttgtc gacgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
     4501 cctcgagtta attaacgaga gcataatatt gatatgtgcc aaagttgttt ctgactgact
     4561 aataagtata atttgtttct attatgtata ggttaagcta attacttatt ttataataca
     4621 acatgactgt ttttaaagta caaaataagt ttatttttgt aaaagagaga atgtttaaaa
     4681 gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac
     4741 ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta
     4801 atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa
     4861 ttttacgcat gattatcttt aacgtacact actagggaca ggattggtga cagaaaagcc
     4921 ccatccttag gcctcctcct tcctagtctc ctgatattgg gtctaacccc cacctcctgt
     4981 taggcagatt ccttatctgg tgacacaccc ccatttcctg gagccatctc tctccttgcc
     5041 agaacctcta aggtttgctt acgatggagc cagagaggat cctgggaggg agagcttggc
     5101 agggggtggg agggaagggg gggatgcgtg acctgcccgg ttctcagtgg ccaccctgcg
     5161 ctaccctctc ccagaacctg agctgctctg acgcggctgt ctggtgcgtt tcactgatcc
     5221 tggtgctgca gcttccttac acttcccaag aggagaagca gtttggaaaa acaaaatcag
     5281 aataagttgg tcctgagttc taactttggc tcttcacctt tctagtcccc aatttatatt
     5341 gttcctccgt gcgtcagttt tacctgtgag ataaggccag tagccagccc cgtcctggca
     5401 gggctgtggt gaggaggggg gtgtccgtgt ggaaaactcc ctttgtgaga atggtgcgtc
     5461 ctaggtgttc accaggtcgt ggccgcctct actccctttc tctttctcca tccttctttc
     5521 cttaaagagt ccccagtgct atctgggaca tattcctccg cccagagcag ggtcccgctt
     5581 ccctaaggcc ctgctctggg cttctgggtt tgagtccttg gcaagcccag gagaggcgct
     5641 caggcttccc tgtccccctt cctcgtccac catctcatgc ccctggctct cctgcccctt
     5701 ccctacaggt gttcctggct ctgctctctc gagatgcatg cgtcaatttt acgcagacta
     5761 tctttctagg gttaatctag ctgcatcagg atcatatcgt cgggtctttt ttccggctca
     5821 gtcatcgccc aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc
     5881 gcgaggttga agcggcatgg aaagagtttg ccgaggatga attatgccca gtacatgacc
     5941 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     6001 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     6061 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     6121 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     6181 gaggtttata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     6241 cgctgttttg acctccatag aagattctag aatggccctt agtaagcacg gcctgactaa
     6301 ggacatgacc atgaagtacc atatggaggg ctcggtggac gggcacaagt tcgtgattac
     6361 gggccacggg aacggcaacc cattcgaggg gaaacagaca atgaacctgt gtgtggtgga
     6421 aggcggaccc ctgcccttct cggaggacat cctgtccgca acctttgact acggcaatcg
     6481 cgtgttcaca gagtatccac agggtatggt ggatttcttt aagaacagct gtcccgcagg
     6541 atacacctgg caccgcagcc tgctcttcga ggacggggcg gtgtgtacca cctccgctga
     6601 tatcaccgtg tccgtcgagg agaactgctt ctaccacaac agcaaattcc acggcgtgaa
     6661 ctttcctgcc gacgggcctg tgatgaagaa aatgactacg aattgggagc ccagctgcga
     6721 gaaaatcatc ccggtccctc gtcagggaat cttgaagggc gacatcgcaa tgtacctcct
     6781 gctcaaggac gggggaagat accgatgcca gttcgatacc atctacaagg caaagtccga
     6841 tcccaaggag atgccagagt ggcacttcat ccagcacaaa ctcacccgcg aggaccgctc
     6901 agatgccaag aaccagaagt ggcaactggt cgagcacgcc gtggcatccc gtagcgccct
     6961 gccagggtaa ttcgaattta aatcggatcc ctgtgccttc tagttgccag ccatctgttg
     7021 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7081 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7141 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7201 cggtgggctc tatggagatc tgcggccgcg aaggatctgc gatcgctccg gtgcccgtca
     7261 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     7321 aacgggtgcc tagagaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7381 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7441 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7501 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7561 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7621 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7681 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7741 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7801 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7861 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7921 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7981 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8041 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8101 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8161 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8221 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8281 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8341 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8401 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8461 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8521 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8581 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8641 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8701 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8761 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8821 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8881 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8941 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9001 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9061 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9121 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     9181 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     9241 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     9301 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     9361 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     9421 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     9481 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     9541 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     9601 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     9661 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     9721 ttgtaaaacg acggccagtg agcgcgcctc gttcattcac gtttttgaac ccgtggagga
     9781 cgggcagact cgcggtgcaa atgtgtttta cagcgtgatg gagcagatga agatgctcga
     9841 cacgctgcag aacacgcagc tagattaacc ctagaaagat aatcatattg tgacgtacgt
     9901 taaagataat catgtgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta
     9961 tttattaatt tgaatagata ttaagtttta ttatatttac acttacatac taataataaa
    10021 ttcaacaaac aattta
//
