LOCUS       sequence-396465-        6414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BACH1 C-terminus truncated isoform - BACH1t expression plasmid with 
            a RFP reporter on the backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Wan Y, Cohen J, Szenk M, Farquhar KS, Coraci D, Krzyszton R, Azukas 
            J, Van Nest N, Smashnov A, Chern YJ, De Martino D, Nguyen LC, Bien 
            H, Bravo-Cordero JJ, Chan CH, Rosner MR, Balazsi G
  TITLE     Nonmonotone invasion landscape by noise-aware control of metastasis 
            activator levels.
  JOURNAL   Nat Chem Biol. 2023 Jul;19(7):887-899. doi: 
            10.1038/s41589-023-01344-z. Epub 2023 May 25.
  PUBMED    37231268
REFERENCE   2  (bases 1 to 6414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-023-01344-z"; journalName: "Nat Chem Biol"; date: 
            "2023-07"; volume: "19"; issue: "7"; pages: "887-899"
COMMENT     Sequence Label: pCMV-BACH1t_RFP
FEATURES             Location/Qualifiers
     source          1..6414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        37..416
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        417..620
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     571..591
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     665..684
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        665..683
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2620..2638)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2621..2638)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2658..2675)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2664..2888
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2916..2950
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        3051..3550
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3086..3109)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3483..3502
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3590..3599
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4315..4539
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4700..5288)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4780..4799)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5459..6250,6251..6319))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6082..6101
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6320..6414,1..10))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggttccgcg cacatttccc gccagatata cgcgttgaca ttgattattg actagttatt
       61 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      121 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      181 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      241 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      301 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      361 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      421 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      481 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      541 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      601 aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg
      661 aaattaatac gactcactat agggagaccc aagcttggta ccgagctcgg atccaccggt
      721 cgccaccatg tctctgagtg agaactcggt ttttgcctat gaatcttctg tgcatagcac
      781 caatgtttta ctcagcctta atgaccagcg gaagaaagat gtgctgtgcg atgtcaccat
      841 ctttgtggag ggacagcggt tccgcgctca ccggtccgtg ctggcggcat gcagcagtta
      901 cttccactca agaatcgtag gccaggctga tggagagctg aacattactc ttccagaaga
      961 ggtgacagtt aaaggatttg aacctttaat tcagtttgcc tacactgcta aactgatttt
     1021 aagtaaagag aatgtggatg aagtgtgcaa atgtgtggag tttttaagtg tacataatat
     1081 tgaggaatcc tgctttcagt ttctgaaatt taagtttttg gactccactg cagaccagca
     1141 agaatgccca agaaaaaaat gcttttcatc acactgtcag aaaacagacc ttaaactttc
     1201 acttttggac cagagggatc tagaaactga tgaagtggag gaatttctgg aaaataaaaa
     1261 tgttcagact cctcagtgta aactccgcag gtatcaagga aatgcaaaag cctcacctcc
     1321 tctacaagac agtgccagtc agacatatga gtccatgtgc ttagagaagg atgctgctct
     1381 ggccttgcct tctttatgcc ccaaatacag aaaattccaa aaagcatttg gaactgacag
     1441 agtccgtact ggggaatcta gtgtcaaaga cattcatgct tctgttcagc caaatgaaag
     1501 gtctgaaaat gaatgcctgg gaggagtccc ggagtgtaga gatttgcagg tgatgttaaa
     1561 atgtgacgaa agtaaattag caatggaacc tgaagaaacg aagaaagatc ctgcttctca
     1621 gtgcccaact gaaaaatcag aagtgactcc tttcccccac aattcttcca tagaccctca
     1681 tggactttat tctttgtctc ttttacacac atatgaccaa tatggtgact tgaattttgc
     1741 tggtatgcaa aacacaacag tgttaacaga aaagcctttg tcaggtacag acgtccaaga
     1801 aaaaacattt ggtgaaagtc aggatttacc tttgaaatcc gacttgggca ccagggaaga
     1861 tagtagtgtt gcatctagtg ataggagtag tgtggagcga gaagtggcag aacacctagc
     1921 aaaaggcttc tggagtgaca tttgcagcac ggacactcct tgccaaatgc agttatcacc
     1981 tgctgtggcc aaagatggct cagaacagat ctcacagaaa cggtctgagt gtccgtggtt
     2041 aggtatcagg attagtgaga gcccagaacc aggtcaaagg actttcacaa cattaagttc
     2101 tgtcaactgc ccttttataa gtactctgag tactgaaggc tgttcaagca atttggaaat
     2161 tggaaacgat gattatgttt cagaacccca gcaagaacct tgcccatatg cttgtgtcat
     2221 tagcttggga gacgactctg agacggacac cgaaggagac agtgaatcct gttcagccag
     2281 agaacaagaa tgtgaggtaa aactgccatt caatgcacaa cggataattt cactgtctcg
     2341 aaatgatttt cagtccttgt tgaaaatgca caagcttact ccagaacagc tggattgtat
     2401 ccatgatatt cgaagaagaa gtaaaaacag aattgctgca cagcgctgtc gcaagagaaa
     2461 acttgactgt atacagaatc ttgaatcaga aattgagaag ctgggttctg tggaaaatct
     2521 gatgtgccaa gaagtgttcc ataccattct gagaactctg gatacctgct cagacagctc
     2581 tgtttcagct aaacagtaag cgactctaga gggccctatt ctatagtgtc acctaaatgc
     2641 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     2701 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     2761 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     2821 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     2881 ctctatggct tgtcgacgaa ggctctctcg agatgcatgc gtcaatttta cgcagactat
     2941 ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt tccggctcag
     3001 tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg gggtagggga
     3061 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     3121 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     3181 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     3241 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     3301 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     3361 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgtgtccgg
     3421 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     3481 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     3541 gggcctttcg acctgcagcg acccgcttaa cagcgtcaac agcgtgccgg ccaccatggc
     3601 ccttagtaag cacggcctga ctaaggacat gaccatgaag taccatatgg agggctcggt
     3661 ggacgggcac aagttcgtga ttacgggcca cgggaacggc aacccattcg aggggaaaca
     3721 gacaatgaac ctgtgtgtgg tggaaggcgg acccctgccc ttctcggagg acatcctgtc
     3781 cgcaaccttt gactacggca atcgcgtgtt cacagagtat ccacagggta tggtggattt
     3841 ctttaagaac agctgtcccg caggatacac ctggcaccgc agcctgctct tcgaggacgg
     3901 ggcggtgtgt accacctccg ctgatatcac cgtgtccgtc gaggagaact gcttctacca
     3961 caacagcaaa ttccacggcg tgaactttcc tgccgacggg cctgtgatga agaaaatgac
     4021 tacgaattgg gagcccagct gcgagaaaat catcccggtc cctcgtcagg gaatcttgaa
     4081 gggcgacatc gcaatgtacc tcctgctcaa ggacggggga agataccgat gccagttcga
     4141 taccatctac aaggcaaagt ccgatcccaa ggagatgcca gagtggcact tcatccagca
     4201 caaactcacc cgcgaggacc gctcagatgc caagaaccag aagtggcaac tggtcgagca
     4261 cgccgtggca tcccgtagcg ccctgccagg gtaattcgaa tttaaatcgg atccctgtgc
     4321 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4381 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4441 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4501 acaatagcag gcatgctggg gatgcggtgg gctctatgga gatctgcggc cgcgaaggat
     4561 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     4621 gttgggggga ggggtcggca attgaacggg tgcctagaga aaaggccagg aaccgtaaaa
     4681 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4741 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4801 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4861 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4921 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4981 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5041 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5101 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5161 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5221 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5281 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5341 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5401 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5461 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5521 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5581 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5641 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5701 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5761 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5821 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5881 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5941 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6001 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6061 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6121 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6181 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6241 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6301 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa atag
//
