LOCUS       sequence-396470-        9926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1
            Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR 
            for positive selection and HSV-TK for negative selection. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9926)
  AUTHORS   Wan Y, Cohen J, Szenk M, Farquhar KS, Coraci D, Krzyszton R, Azukas 
            J, Van Nest N, Smashnov A, Chern YJ, De Martino D, Nguyen LC, Bien 
            H, Bravo-Cordero JJ, Chan CH, Rosner MR, Balazsi G
  TITLE     Nonmonotone invasion landscape by noise-aware control of metastasis 
            activator levels.
  JOURNAL   Nat Chem Biol. 2023 Jul;19(7):887-899. doi: 
            10.1038/s41589-023-01344-z. Epub 2023 May 25.
  PUBMED    37231268
REFERENCE   2  (bases 1 to 9926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-023-01344-z"; journalName: "Nat Chem Biol"; date: 
            "2023-07"; volume: "19"; issue: "7"; pages: "887-899"
COMMENT     Sequence Label: pUt-mNF-BACH1 Cas9-Resistant
FEATURES             Location/Qualifiers
     source          1..9926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    19..52
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     enhancer        73..452
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        453..656
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    652..670
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    678..696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          879..1451
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1463..1472
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2120..2176
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(2177..2179,2180..2182,2183..2893)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2177..2179,2180..2182,2183..2893)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2222..2243)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2483..2502)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5196..5216)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(5569..5586)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5575..5799
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    5934..6328
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     promoter        complement(6769..6787)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6779..6825)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6872..7074)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        7076..7379
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    7423..7470
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     rep_origin      complement(7482..8070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7562..7581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8103..8602
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8138..8161)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8535..8554
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     5'UTR           8644..8683
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             8701..9831
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(9829..9848)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9873..9921
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 cgcggaagtt cctattccga agttcctatt cttcaaatag tataggaact tcacgggcca
       61 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      121 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      181 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      241 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      301 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      361 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      421 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      481 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      541 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      601 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc atccctatca
      661 gtgatagaga tcagatctcc ctatcagtga tagagagctg ttttgacctc catagaagac
      721 accgggaccg atccagcctc cggactctag cgtttaaact taagcttggt acccggggat
      781 cctctagggc ctctgagcta ttccagaagt agtgaagagg cttttttgga ggcctaggct
      841 tttgcaaaaa gctccggatc gatcctgaga acttcagggt gagtttgggg acccttgatt
      901 gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg
      961 tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt
     1021 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg
     1081 taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt
     1141 atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat
     1201 tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa
     1261 tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca
     1321 tcctggtcat catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga
     1381 ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct
     1441 ttttcctaca ggtcctgcag gcgccaccat gggttctaga ctggacaaga gcaaagtcat
     1501 aaactctgct ctggaattac tcaatgaagt cggtatcgaa ggcctgacga caaggaaact
     1561 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
     1621 cctgctcgat gccctggcaa tcgagatgct ggacaggcat catacccact tctgccccct
     1681 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcattcc gctgtgctct
     1741 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     1801 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     1861 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggatcagga
     1921 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     1981 gagacaagca attgagctgt tcgaccatca gggagccgaa cctgccttcc ttttcggcct
     2041 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccaaaaaa
     2101 gaagagaaag ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga
     2161 ggagaaccct ggacctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     2221 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2281 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2341 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     2401 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2461 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2521 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2581 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     2641 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     2701 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2761 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     2821 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     2881 cgagctgtac aagggtgacg gtgctggttt aattaacatg tctctgagtg agaactcggt
     2941 ttttgcctat gaatcttctg tgcatagcac caatgtttta ctcagcctta atgaccagcg
     3001 gaagaaagat gtgctgtgcg atgtcaccat ctttgtggag ggacagcggt tccgcgctca
     3061 ccggtccgtg ctggcggcat gcagcagtta ctttcactca agaatcgtag gccaggctga
     3121 tggagagctg aacattactc ttccagaaga ggtgacagtt aaaggatttg aacctttaat
     3181 tcagtttgcc tacactgcta aactgatttt aagtaaagag aatgtggatg aagtgtgcaa
     3241 atgtgtggag tttttaagtg tacataatat tgaggaatcc tgctttcagt ttctgaaatt
     3301 taagtttttg gactccactg cagaccagca agaatgccca agaaaaaaat gcttttcatc
     3361 acactgtcag aaaacagacc ttaaactttc acttttggac cagagggatc tagaaactga
     3421 tgaagtggag gaatttctgg aaaataaaaa tgttcagact cctcagtgta aactccgcag
     3481 gtatcaagga aatgcaaaag cctcacctcc tctacaagac agtgccagtc agacatatga
     3541 gtccatgtgc ttagagaagg atgctgctct ggccttgcct tctttatgcc ccaaatacag
     3601 aaaattccaa aaagcatttg gaactgacag agtccgtact ggggaatcta gtgtcaaaga
     3661 cattcatgct tctgttcagc caaatgaaag gtctgaaaat gaatgcctgg gaggagtccc
     3721 ggagtgtaga gatttgcagg tgatgttaaa atgtgacgaa agtaaattag caatggaacc
     3781 tgaagaaacg aagaaagatc ctgcttctca gtgcccaact gaaaaatcag aagtgactcc
     3841 tttcccccac aattcttcca tagaccctca tggactttat tctttgtctc ttttacacac
     3901 atatgaccaa tatggtgact tgaattttgc tggtatgcaa aacacaacag tgttaacaga
     3961 aaagcctttg tcaggtacag acgtccaaga aaaaacattt ggtgaaagtc aggatttacc
     4021 tttgaaatcc gacttgggca ccagggaaga tagtagtgtt gcatctagtg ataggagtag
     4081 tgtggagcga gaagtggcag aacacctagc aaaaggcttc tggagtgaca tttgcagcac
     4141 ggacactcct tgccaaatgc agttatcacc tgctgtggcc aaagatggct cagaacagat
     4201 ctcacagaaa cggtctgagt gtccgtggtt aggtatcagg attagtgaga gcccagaacc
     4261 aggtcaaagg actttcacaa cattaagttc tgtcaactgc ccttttataa gtactctgag
     4321 tactgaaggc tgttcaagca atttggaaat tggaaacgat gattatgttt cagaacccca
     4381 gcaagaacct tgcccatatg cttgtgtcat tagcttggga gacgactctg agacggacac
     4441 cgaaggagac agtgaatcct gttcagccag agaacaagaa tgtgaggtaa aactgccatt
     4501 caatgcacaa cggataattt cactgtctcg aaatgatttt cagtccttgt tgaaaatgca
     4561 caagcttact ccagaacagc tggattgtat ccatgatatt cgaagaagaa gtaaaaacag
     4621 aattgctgca cagcgctgtc gcaagagaaa acttgactgt atacagaatc ttgaatcaga
     4681 aattgagaag ctgcaaagtg aaaaggagag cttgttgaag gaaagagatc acattttgtc
     4741 aactctgggt gagacaaagc agaacctaac tggactttgc cagaaagttt gtaaagaagc
     4801 agctctgagt caagaacaaa tacagatact cgccaagtac tcagctgcag attgcccact
     4861 ttcattttta atttctgaaa aagataaaag tactcctgat ggtgaactgg cgttaccatc
     4921 aattttcagt ttatctgacc ggcctccagc agtgctgcct ccctgtgcca gaggaaacag
     4981 tgagcctggc tacgcgcgag ggcaggagtc ccagcagatg tccacagcca cctctgagca
     5041 agctgggcct gcggaacagt gtcgtcagag tggtgggatc tcagatttct gtcagcagat
     5101 gactgataaa tgtactactg atgagtaaac cggtgcggcc gcaatcaacc tctggattac
     5161 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5221 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5281 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5341 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5401 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5461 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5521 gtggtgttgt cctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     5581 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5641 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5701 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5761 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     5821 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5881 tggtggttac gcgctaaagc aagtaaaacc tctacaaatg tggtatggaa ttctaaaata
     5941 cagcatagca aaactttaac ctccaaatca agcctctact tgaatccttt tctgagggat
     6001 gaataaggca taggcatcag gggctgttgc caatgtgcat tagctgtttg cagcctcacc
     6061 ttctttcatg gagtttaaga tatagtgtat tttcccaagg tttgaactag ctcttcattt
     6121 ctttatgttt taaatgcact gacctcccac attccctttt tagtaaaata ttcagaaata
     6181 atttaaatac atcattgcaa tgaaaataaa tgttttttat taggcagaat ccagatgctc
     6241 aaggcccttc ataatatccc ccagtttagt agttggactt agggaacaaa ggaaccttta
     6301 atagaaattg gacagcaaga aagcgagctt ctagctttag ttcctggtgt acttgagggg
     6361 gatgagttcc tcaatggtgg ttttgaccag cttgccattc atctcaatga gcacaaagca
     6421 gtcaggagca tagtcagaga tgagctctct gcacatgcca caggggctga ccaccctgat
     6481 ggatctgtcc acctcatcag agtaggggtg cctgacagcc acaatggtgt caaagtcctt
     6541 ctgcccgttg ctcacagcag acccaatggc aatggcttca gcacagacag tgaccctgcc
     6601 aatgtaggcc tcaatgtgga cagcagagat gatctcccca gtcttggtcc tgatggccgc
     6661 cccgacatgg tgcttgttgt cctcatagag catggtgatc ttctcagtgg cgacctccac
     6721 cagctccaga tcctgctgag agatgttgaa ggtcttcatg gtggccctcc tatagtgagt
     6781 cgtattatac tatgccgata tactatgccg atgattaatt gtcaaaacag cgtggatggc
     6841 gtctccagct tatctgacgg ttcactaaac gagctctgct tatatagacc tcccaccgta
     6901 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     6961 gttgatttac tagtcaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     7021 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcatcatgg
     7081 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     7141 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     7201 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     7261 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     7321 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     7381 ctgcaggtta attaagaaca tgtgagcaaa aggccagcaa aagaagttcc tattccgaag
     7441 ttcctattct ctagaaagta taggaacttc ttgctggcgt ttttccatag gctccgcccc
     7501 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7561 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7621 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7681 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7741 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7801 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7861 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7921 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7981 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8041 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggttcta
     8101 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     8161 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
     8221 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     8281 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
     8341 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
     8401 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
     8461 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     8521 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     8581 ttcctcatct ccgggccttt cgacctgcag cgacccgctt aacagcgtca acagcgtgcc
     8641 gcagatcttg gtggcgtgaa actcccgcac ctcttcggca agcgccttgt agaagcgcgt
     8701 atggcttcgt acccctgcca tcaacacgcg tctgcgttcg accaggctgc gcgttctcgc
     8761 ggccatagca accgacgtac ggcgttgcgc cctcgccggc agcaagaagc cacggaagtc
     8821 cgcctggagc agaaaatgcc cacgctactg cgggtttata tagacggtcc tcacgggatg
     8881 gggaaaacca ccaccacgca actgctggtg gccctgggtt cgcgcgacga tatcgtctac
     8941 gtacccgagc cgatgactta ctggcaggtg ctgggggctt ccgagacaat cgcgaacatc
     9001 tacaccacac aacaccgcct cgaccagggt gagatatcgg ccggggacgc ggcggtggta
     9061 atgacaagcg cccagataac aatgggcatg ccttatgccg tgaccgacgc cgttctggct
     9121 cctcatatcg ggggggaggc tgggagctca catgccccgc ccccggccct caccctcatc
     9181 ttcgaccgcc atcccatcgc cgccctcctg tgctacccgg ccgcgcgata ccttatgggc
     9241 agcatgaccc cccaggccgt gctggcgttc gtggccctca tcccgccgac cttgcccggc
     9301 acaaacatcg tgttgggggc ccttccggag gacagacaca tcgaccgcct ggccaaacgc
     9361 cagcgccccg gcgagcggct tgacctggct atgctggccg cgattcgccg cgtttacggg
     9421 ctgcttgcca atacggtgcg gtatctgcag ggcggcgggt cgtggcggga ggattgggga
     9481 cagctttcgg ggacggccgt gccgccccag ggtgccgagc cccagagcaa cgcgggccca
     9541 cgaccccata tcggggacac gttatttacc ctgtttcggg cccccgagtt gctggccccc
     9601 aacggcgacc tgtacaacgt gtttgcctgg gccttggacg tcttggccaa acgcctccgt
     9661 cccatgcacg tctttatcct ggattacgac caatcgcccg ccggctgccg ggacgccctg
     9721 ctgcaactta cctccgggat ggtccagacc cacgtcacca cccccggctc cataccgacg
     9781 atctgcgacc tggcgcgcac gtttgcccgg gagatggggg aggctaactg aaacacggaa
     9841 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     9901 cgggtgttgg gtcgtttgtt cataaa
//
