LOCUS       sequence-396475-        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human cytosolic malate dehydrogenase isoform
            1 transcript variant 3 (hMDH1v3); Use in CURES (integration of 
            research into undergraduate teaching labs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  TITLE     Expression of His tagged recombinant malate dehydrogenase (MDH)
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET28a hMDH1v3 (+TEV)
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1066..1086
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1087..1104
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtcgac gctgcagcta ttttccaaag gacgttacgg tgtttgataa ggacgataag
       61 tctgaaccaa tcagagtcct tgtgactgga gcagctggtc aaattgcata ttcactgctg
      121 tacagtattg gaaatggatc tgtctttggt aaagatcagc ctataattct tgtgctgttg
      181 gatatcaccc ccatgatggg tgtcctggac ggtgtcctaa tggaactgca agactgtgcc
      241 cttcccctcc tgaaagatgt catcgcaaca gataaagaag acgttgcctt caaagacctg
      301 gatgtggcca ttcttgtggg ctccatgcca agaagggaag gcatggagag aaaagattta
      361 ctgaaagcaa atgtgaaaat cttcaaatcc cagggtgcag ccttagataa atacgccaag
      421 aagtcagtta aggttattgt tgtgggtaat ccagccaata ccaactgcct gactgcttcc
      481 aagtcagctc catccatccc caaggagaac ttcagttgct tgactcgttt ggatcacaac
      541 cgagctaaag ctcaaattgc tcttaaactt ggtgtgactg ctaatgatgt aaagaatgtc
      601 attatctggg gaaaccattc ctcgactcag tatccagatg tcaaccatgc caaggtgaaa
      661 ttgcaaggaa aggaagttgg tgtttatgaa gctctgaaag atgacagctg gctcaaggga
      721 gaatttgtca cgactgtgca gcagcgtggc gctgctgtca tcaaggctcg aaaactatcc
      781 agtgccatgt ctgctgcaaa agccatctgt gaccacgtca gggacatctg gtttggaacc
      841 ccagagggag agtttgtgtc catgggtgtt atctctgatg gcaactccta tggtgttcct
      901 gatgatctgc tctactcatt ccctgttgta atcaagaata agacctggaa gtttgttgaa
      961 ggtctcccta ttaatgattt ctcacgtgag aagatggatc ttactgcaaa ggaactgaca
     1021 gaagaaaaag aaagtgcttt tgaatttctt tcctctgccc tcgaggagaa cctgtacttc
     1081 cagggtcacc accaccacca ccactga
//
